UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i>
Epigenetic modification is important for cellular functions. Trimethylation of histone H3 lysine 4 (H3K4me3), which associates with transcriptional activation, is one of the important epigenetic modifications. In this study, the biological functions of UvKmt2-mediated H3K4me3 modification were chara...
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MDPI AG
2022-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/6/553 |
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| author | Shuai Meng Huanbin Shi Chuyu Lin Zhongling Wu Fucheng Lin Zeng Tao Yanjun Kou |
| author_facet | Shuai Meng Huanbin Shi Chuyu Lin Zhongling Wu Fucheng Lin Zeng Tao Yanjun Kou |
| author_sort | Shuai Meng |
| collection | DOAJ |
| description | Epigenetic modification is important for cellular functions. Trimethylation of histone H3 lysine 4 (H3K4me3), which associates with transcriptional activation, is one of the important epigenetic modifications. In this study, the biological functions of UvKmt2-mediated H3K4me3 modification were characterized in <i>Ustilaginoidea virens</i>, which is the causal agent of the false smut disease, one of the most destructive diseases in rice. Phenotypic analyses of the Δ<i>Uvkmt2</i> mutant revealed that <i>UvKMT2</i> is necessary for growth, conidiation, secondary spore formation, and virulence in <i>U. virens</i>. Immunoblotting and chromatin immunoprecipitation assay followed by sequencing (ChIP-seq) showed that <i>UvKMT2</i> is required for the establishment of H3K4me3, which covers 1729 genes of the genome in <i>U. virens</i>. Further RNA-seq analysis demonstrated that UvKmt2-mediated H3K4me3 acts as an important role in transcriptional activation. In particular, H3K4me3 modification involves in the transcriptional regulation of conidiation-related and pathogenic genes, including two important mitogen-activated protein kinases <i>UvHOG1</i> and <i>UvPMK1</i>. The down-regulation of <i>UvHOG1</i> and <i>UvPMK1</i> genes may be one of the main reasons for the reduced pathogenicity and stresses adaptability of the ∆<i>Uvkmt2</i> mutant. Overall, H3K4me3, established by histone methyltransferase <i>UvKMT2</i>, contributes to fungal development, secondary spore formation, virulence, and various stress responses through transcriptional regulation in <i>U. virens</i>. |
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| language | English |
| last_indexed | 2024-03-09T23:22:13Z |
| publishDate | 2022-05-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-2e315958f2984a3ca08a80f6bb124b732023-11-23T17:23:59ZengMDPI AGJournal of Fungi2309-608X2022-05-018655310.3390/jof8060553UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i>Shuai Meng0Huanbin Shi1Chuyu Lin2Zhongling Wu3Fucheng Lin4Zeng Tao5Yanjun Kou6State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Lab of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Lab of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Lab of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaEpigenetic modification is important for cellular functions. Trimethylation of histone H3 lysine 4 (H3K4me3), which associates with transcriptional activation, is one of the important epigenetic modifications. In this study, the biological functions of UvKmt2-mediated H3K4me3 modification were characterized in <i>Ustilaginoidea virens</i>, which is the causal agent of the false smut disease, one of the most destructive diseases in rice. Phenotypic analyses of the Δ<i>Uvkmt2</i> mutant revealed that <i>UvKMT2</i> is necessary for growth, conidiation, secondary spore formation, and virulence in <i>U. virens</i>. Immunoblotting and chromatin immunoprecipitation assay followed by sequencing (ChIP-seq) showed that <i>UvKMT2</i> is required for the establishment of H3K4me3, which covers 1729 genes of the genome in <i>U. virens</i>. Further RNA-seq analysis demonstrated that UvKmt2-mediated H3K4me3 acts as an important role in transcriptional activation. In particular, H3K4me3 modification involves in the transcriptional regulation of conidiation-related and pathogenic genes, including two important mitogen-activated protein kinases <i>UvHOG1</i> and <i>UvPMK1</i>. The down-regulation of <i>UvHOG1</i> and <i>UvPMK1</i> genes may be one of the main reasons for the reduced pathogenicity and stresses adaptability of the ∆<i>Uvkmt2</i> mutant. Overall, H3K4me3, established by histone methyltransferase <i>UvKMT2</i>, contributes to fungal development, secondary spore formation, virulence, and various stress responses through transcriptional regulation in <i>U. virens</i>.https://www.mdpi.com/2309-608X/8/6/553<i>false smut</i>conidiationvirulenceKmt2H3K4me3rice |
| spellingShingle | Shuai Meng Huanbin Shi Chuyu Lin Zhongling Wu Fucheng Lin Zeng Tao Yanjun Kou UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i> Journal of Fungi <i>false smut</i> conidiation virulence Kmt2 H3K4me3 rice |
| title | UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i> |
| title_full | UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i> |
| title_fullStr | UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i> |
| title_full_unstemmed | UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i> |
| title_short | UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in <i>Ustilaginoidea virens</i> |
| title_sort | uvkmt2 mediated h3k4 trimethylation is required for pathogenicity and stress response in i ustilaginoidea virens i |
| topic | <i>false smut</i> conidiation virulence Kmt2 H3K4me3 rice |
| url | https://www.mdpi.com/2309-608X/8/6/553 |
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