Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae
Abstract Background Most embryonic loss in pigs occurs before d 30 of gestation. Dietary supplementation with L-arginine (Arg) during early gestation can enhance the survival and development of conceptuses (embryo/fetus and its extra-embryonic membranes) in gilts. However, the underlying mechanisms...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-12-01
|
| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40104-022-00794-0 |
| _version_ | 1811204183167074304 |
|---|---|
| author | Cassandra M. Herring Fuller W. Bazer Gregory A. Johnson Heewon Seo Shengdi Hu Mohammed Elmetwally Wenliang He Daniel B. Long Guoyao Wu |
| author_facet | Cassandra M. Herring Fuller W. Bazer Gregory A. Johnson Heewon Seo Shengdi Hu Mohammed Elmetwally Wenliang He Daniel B. Long Guoyao Wu |
| author_sort | Cassandra M. Herring |
| collection | DOAJ |
| description | Abstract Background Most embryonic loss in pigs occurs before d 30 of gestation. Dietary supplementation with L-arginine (Arg) during early gestation can enhance the survival and development of conceptuses (embryo/fetus and its extra-embryonic membranes) in gilts. However, the underlying mechanisms remain largely unknown. Methods Between d 14 and 30 of gestation, each gilt was fed daily 2 kg of a corn- and soybean-meal based diet (12% crude protein) supplemented with either 0.4% Arg (as Arg-HCl) or an isonitrogenous amount of L-alanine (Control). There were 10 gilts per treatment group. On d 30 of gestation, gilts were fed either Arg-HCl or L-alanine 30 min before they were hysterectomized, followed by the collection of placentae, embryos, fetal membranes, and fetal fluids. Amniotic and allantoic fluids were analyzed for nitrite and nitrate [NOx; stable oxidation products of nitric oxide (NO)], polyamines, and amino acids. Placentae were analyzed for syntheses of NO and polyamines, water and amino acid transport, concentrations of amino acid-related metabolites, and the expression of angiogenic factors and aquaporins (AQPs). Results Compared to the control group, Arg supplementation increased (P < 0.05) the number of viable fetuses by 1.9 per litter, the number and diameter of placental blood vessels (+ 25.9% and + 17.0% respectively), embryonic survival (+ 18.5%), total placental weight (+ 36.5%), the total weight of viable fetuses (+ 33.5%), fetal crown-to-rump length (+ 4.7%), and total allantoic and amniotic fluid volumes (+ 44.6% and + 75.5% respectively). Compared to control gilts, Arg supplementation increased (P < 0.05) placental activities of GTP cyclohydrolase-1 (+ 33.1%) and ornithine decarboxylase (+ 29.3%); placental syntheses of NO (+ 26.2%) and polyamines (+ 28.9%); placental concentrations of NOx (+ 22.5%), tetrahydrobiopterin (+ 21.1%), polyamines (+ 20.4%), cAMP (+ 27.7%), and cGMP (+ 24.7%); total amounts of NOx (+ 61.7% to + 96.8%), polyamines (+ 60.7% to + 88.7%), amino acids (+ 39% to + 118%), glucose (+ 60.5% to + 62.6%), and fructose (+ 41.4% to + 57.0%) in fetal fluids; and the placental transport of water (+ 33.9%), Arg (+ 78.4%), glutamine (+ 89.9%), and glycine (+ 89.6%). Furthermore, Arg supplementation increased (P < 0.05) placental mRNA levels for angiogenic factors [VEGFA120 (+ 117%), VEGFR1 (+ 445%), VEGFR2 (+ 373%), PGF (+ 197%), and GCH1 (+ 126%)] and AQPs [AQP1 (+ 280%), AQP3 (+ 137%), AQP5 (+ 172%), AQP8 (+ 165%), and AQP9 (+ 127%)]. Conclusion Supplementing 0.4% Arg to a conventional diet for gilts between d 14 and d 30 of gestation enhanced placental NO and polyamine syntheses, angiogenesis, and water and amino acid transport to improve conceptus development and survival. |
| first_indexed | 2024-04-12T03:06:57Z |
| format | Article |
| id | doaj.art-2e336591155e4bd18a2fdba56f790fbc |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-04-12T03:06:57Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-2e336591155e4bd18a2fdba56f790fbc2022-12-22T03:50:28ZengBMCJournal of Animal Science and Biotechnology2049-18912022-12-0113111310.1186/s40104-022-00794-0Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentaeCassandra M. Herring0Fuller W. Bazer1Gregory A. Johnson2Heewon Seo3Shengdi Hu4Mohammed Elmetwally5Wenliang He6Daniel B. Long7Guoyao Wu8Department of Animal Science, Texas A&M UniversityDepartment of Animal Science, Texas A&M UniversityDepartment of Veterinary Integrative Biosciences, Texas A&M UniversityDepartment of Veterinary Integrative Biosciences, Texas A&M UniversityDepartment of Animal Science, Texas A&M UniversityDepartment of Animal Science, Texas A&M UniversityDepartment of Animal Science, Texas A&M UniversityDepartment of Animal Science, Texas A&M UniversityDepartment of Animal Science, Texas A&M UniversityAbstract Background Most embryonic loss in pigs occurs before d 30 of gestation. Dietary supplementation with L-arginine (Arg) during early gestation can enhance the survival and development of conceptuses (embryo/fetus and its extra-embryonic membranes) in gilts. However, the underlying mechanisms remain largely unknown. Methods Between d 14 and 30 of gestation, each gilt was fed daily 2 kg of a corn- and soybean-meal based diet (12% crude protein) supplemented with either 0.4% Arg (as Arg-HCl) or an isonitrogenous amount of L-alanine (Control). There were 10 gilts per treatment group. On d 30 of gestation, gilts were fed either Arg-HCl or L-alanine 30 min before they were hysterectomized, followed by the collection of placentae, embryos, fetal membranes, and fetal fluids. Amniotic and allantoic fluids were analyzed for nitrite and nitrate [NOx; stable oxidation products of nitric oxide (NO)], polyamines, and amino acids. Placentae were analyzed for syntheses of NO and polyamines, water and amino acid transport, concentrations of amino acid-related metabolites, and the expression of angiogenic factors and aquaporins (AQPs). Results Compared to the control group, Arg supplementation increased (P < 0.05) the number of viable fetuses by 1.9 per litter, the number and diameter of placental blood vessels (+ 25.9% and + 17.0% respectively), embryonic survival (+ 18.5%), total placental weight (+ 36.5%), the total weight of viable fetuses (+ 33.5%), fetal crown-to-rump length (+ 4.7%), and total allantoic and amniotic fluid volumes (+ 44.6% and + 75.5% respectively). Compared to control gilts, Arg supplementation increased (P < 0.05) placental activities of GTP cyclohydrolase-1 (+ 33.1%) and ornithine decarboxylase (+ 29.3%); placental syntheses of NO (+ 26.2%) and polyamines (+ 28.9%); placental concentrations of NOx (+ 22.5%), tetrahydrobiopterin (+ 21.1%), polyamines (+ 20.4%), cAMP (+ 27.7%), and cGMP (+ 24.7%); total amounts of NOx (+ 61.7% to + 96.8%), polyamines (+ 60.7% to + 88.7%), amino acids (+ 39% to + 118%), glucose (+ 60.5% to + 62.6%), and fructose (+ 41.4% to + 57.0%) in fetal fluids; and the placental transport of water (+ 33.9%), Arg (+ 78.4%), glutamine (+ 89.9%), and glycine (+ 89.6%). Furthermore, Arg supplementation increased (P < 0.05) placental mRNA levels for angiogenic factors [VEGFA120 (+ 117%), VEGFR1 (+ 445%), VEGFR2 (+ 373%), PGF (+ 197%), and GCH1 (+ 126%)] and AQPs [AQP1 (+ 280%), AQP3 (+ 137%), AQP5 (+ 172%), AQP8 (+ 165%), and AQP9 (+ 127%)]. Conclusion Supplementing 0.4% Arg to a conventional diet for gilts between d 14 and d 30 of gestation enhanced placental NO and polyamine syntheses, angiogenesis, and water and amino acid transport to improve conceptus development and survival.https://doi.org/10.1186/s40104-022-00794-0AngiogenesisArginineFetusPlacentaReproduction |
| spellingShingle | Cassandra M. Herring Fuller W. Bazer Gregory A. Johnson Heewon Seo Shengdi Hu Mohammed Elmetwally Wenliang He Daniel B. Long Guoyao Wu Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae Journal of Animal Science and Biotechnology Angiogenesis Arginine Fetus Placenta Reproduction |
| title | Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae |
| title_full | Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae |
| title_fullStr | Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae |
| title_full_unstemmed | Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae |
| title_short | Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae |
| title_sort | dietary supplementation with 0 4 l arginine between days 14 and 30 of gestation enhances no and polyamine syntheses and water transport in porcine placentae |
| topic | Angiogenesis Arginine Fetus Placenta Reproduction |
| url | https://doi.org/10.1186/s40104-022-00794-0 |
| work_keys_str_mv | AT cassandramherring dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT fullerwbazer dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT gregoryajohnson dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT heewonseo dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT shengdihu dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT mohammedelmetwally dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT wenlianghe dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT danielblong dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae AT guoyaowu dietarysupplementationwith04largininebetweendays14and30ofgestationenhancesnoandpolyaminesynthesesandwatertransportinporcineplacentae |