BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i>
The study of functional genes involved in baculovirus infection is vital for its wide application in pest biocontrol. This study utilized the <i>Autographa californica</i> nucleopolyhedrovirus (AcMNPV) and silkworm as models to elucidate the role of BmRRS1, which has been found to exhibi...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/306 |
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| author | Liqin Zhou Xinyi Ding Zhisheng Wang Si Zhou Sheng Qin Xia Sun Xueyang Wang Muwang Li |
| author_facet | Liqin Zhou Xinyi Ding Zhisheng Wang Si Zhou Sheng Qin Xia Sun Xueyang Wang Muwang Li |
| author_sort | Liqin Zhou |
| collection | DOAJ |
| description | The study of functional genes involved in baculovirus infection is vital for its wide application in pest biocontrol. This study utilized the <i>Autographa californica</i> nucleopolyhedrovirus (AcMNPV) and silkworm as models to elucidate the role of BmRRS1, which has been found to exhibit notable differential expression between resistant and susceptible silkworm strains. The results showed that it was evolutionarily conserved in selected species. Among different tissues, it was expressed at the highest level in the gonads, followed by the hemolymph and silk glands; among the different developmental stages, it was the highest in the second instar, followed by the pupae and adults. Moreover, its vital role in suppressing AcMNPV infection was verified by the decreased expression of <i>lef3</i> and vp39 protein after overexpression of <i>BmRRS1</i> as well as by the increased expression of the viral gene <i>lef3</i> and the viral protein vp39 after siRNA treatment against <i>BmRRS1</i> expression in BmN cells. Additionally, the direct interaction between BmRRS1 and AcMNPV was detected by the GST pull-down assay. Finally, the homologue of <i>BmRRS1</i> in <i>Spodoptera frugiperda</i> was found to be involved in larval resistance to AcMNPV. In a word, <i>BmRRS1</i> plays a vital role in AcMNPV resistance in silkworms, and this might be related to the direct interaction with AcMNPV. The results of this study provide a potential target for protecting silkworm larvae from virus infection and controlling agricultural and forestry pests. |
| first_indexed | 2024-03-08T15:05:06Z |
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| language | English |
| last_indexed | 2024-03-08T15:05:06Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2e338b391b564e1e8e7d549767cc98082024-01-10T14:58:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125130610.3390/ijms25010306BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i>Liqin Zhou0Xinyi Ding1Zhisheng Wang2Si Zhou3Sheng Qin4Xia Sun5Xueyang Wang6Muwang Li7Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaThe study of functional genes involved in baculovirus infection is vital for its wide application in pest biocontrol. This study utilized the <i>Autographa californica</i> nucleopolyhedrovirus (AcMNPV) and silkworm as models to elucidate the role of BmRRS1, which has been found to exhibit notable differential expression between resistant and susceptible silkworm strains. The results showed that it was evolutionarily conserved in selected species. Among different tissues, it was expressed at the highest level in the gonads, followed by the hemolymph and silk glands; among the different developmental stages, it was the highest in the second instar, followed by the pupae and adults. Moreover, its vital role in suppressing AcMNPV infection was verified by the decreased expression of <i>lef3</i> and vp39 protein after overexpression of <i>BmRRS1</i> as well as by the increased expression of the viral gene <i>lef3</i> and the viral protein vp39 after siRNA treatment against <i>BmRRS1</i> expression in BmN cells. Additionally, the direct interaction between BmRRS1 and AcMNPV was detected by the GST pull-down assay. Finally, the homologue of <i>BmRRS1</i> in <i>Spodoptera frugiperda</i> was found to be involved in larval resistance to AcMNPV. In a word, <i>BmRRS1</i> plays a vital role in AcMNPV resistance in silkworms, and this might be related to the direct interaction with AcMNPV. The results of this study provide a potential target for protecting silkworm larvae from virus infection and controlling agricultural and forestry pests.https://www.mdpi.com/1422-0067/25/1/306AcMNPV<i>Bombyx mori</i><i>BmRRS1</i>siRNA |
| spellingShingle | Liqin Zhou Xinyi Ding Zhisheng Wang Si Zhou Sheng Qin Xia Sun Xueyang Wang Muwang Li BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i> International Journal of Molecular Sciences AcMNPV <i>Bombyx mori</i> <i>BmRRS1</i> siRNA |
| title | BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i> |
| title_full | BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i> |
| title_fullStr | BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i> |
| title_full_unstemmed | BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i> |
| title_short | BmRRS1 Protein Inhibits the Proliferation of Baculovirus <i>Autographa californica</i> Nucleopolyhedrovirus in Silkworm, <i>Bombyx mori</i> |
| title_sort | bmrrs1 protein inhibits the proliferation of baculovirus i autographa californica i nucleopolyhedrovirus in silkworm i bombyx mori i |
| topic | AcMNPV <i>Bombyx mori</i> <i>BmRRS1</i> siRNA |
| url | https://www.mdpi.com/1422-0067/25/1/306 |
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