Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration
Microfluidic preconcentration enables the collection or extraction of low-abundance analytes at specific locations. It has attracted considerable attention as an essential technology in bioengineering, particularly for detection and diagnosis. Herein, we investigated the key parameters in the precon...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/2/398 |
| _version_ | 1797619218804375552 |
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| author | Minsung Kim Bumjoo Kim |
| author_facet | Minsung Kim Bumjoo Kim |
| author_sort | Minsung Kim |
| collection | DOAJ |
| description | Microfluidic preconcentration enables the collection or extraction of low-abundance analytes at specific locations. It has attracted considerable attention as an essential technology in bioengineering, particularly for detection and diagnosis. Herein, we investigated the key parameters in the preconcentration of fluorescent dyes based on electrophoresis in a microfluidic electromembrane system. Commercial ion-exchange membrane (IEM)-integrated polydimethylsiloxane microfluidic devices were fabricated, and Alexa Fluor 488 and Rhodamine 6G were used as fluorescent dyes for sample preconcentration. Through experimental studies, the effect of the channel concentration ratio (CCR, concentration ratio of the main and buffer channels) on the performance of the sample preconcentration was studied. The results show that the preconcentration of the target sample occurs more effectively for a high CCR or high salt concentration of the main channel when the CCR is constant. We also demonstrate a phenomenon that the salt concentration in the electrolyte solution increases as the preconcentration progresses. Our results provide consolidated conditions for electrophoresis-based sample preconcentration in electromembrane systems. |
| first_indexed | 2024-03-11T08:23:54Z |
| format | Article |
| id | doaj.art-2e3477dad73a4102a270653913b12cff |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T08:23:54Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-2e3477dad73a4102a270653913b12cff2023-11-16T22:11:41ZengMDPI AGMicromachines2072-666X2023-02-0114239810.3390/mi14020398Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic MigrationMinsung Kim0Bumjoo Kim1Department of Future Convergence Engineering, Kongju National University, Cheonan 31080, Republic of KoreaDepartment of Future Convergence Engineering, Kongju National University, Cheonan 31080, Republic of KoreaMicrofluidic preconcentration enables the collection or extraction of low-abundance analytes at specific locations. It has attracted considerable attention as an essential technology in bioengineering, particularly for detection and diagnosis. Herein, we investigated the key parameters in the preconcentration of fluorescent dyes based on electrophoresis in a microfluidic electromembrane system. Commercial ion-exchange membrane (IEM)-integrated polydimethylsiloxane microfluidic devices were fabricated, and Alexa Fluor 488 and Rhodamine 6G were used as fluorescent dyes for sample preconcentration. Through experimental studies, the effect of the channel concentration ratio (CCR, concentration ratio of the main and buffer channels) on the performance of the sample preconcentration was studied. The results show that the preconcentration of the target sample occurs more effectively for a high CCR or high salt concentration of the main channel when the CCR is constant. We also demonstrate a phenomenon that the salt concentration in the electrolyte solution increases as the preconcentration progresses. Our results provide consolidated conditions for electrophoresis-based sample preconcentration in electromembrane systems.https://www.mdpi.com/2072-666X/14/2/398preconcentrationelectrophoresision-exchange membranefluorescent dyemicrofluidics |
| spellingShingle | Minsung Kim Bumjoo Kim Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration Micromachines preconcentration electrophoresis ion-exchange membrane fluorescent dye microfluidics |
| title | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
| title_full | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
| title_fullStr | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
| title_full_unstemmed | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
| title_short | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
| title_sort | preconcentration of fluorescent dyes in electromembrane systems via electrophoretic migration |
| topic | preconcentration electrophoresis ion-exchange membrane fluorescent dye microfluidics |
| url | https://www.mdpi.com/2072-666X/14/2/398 |
| work_keys_str_mv | AT minsungkim preconcentrationoffluorescentdyesinelectromembranesystemsviaelectrophoreticmigration AT bumjookim preconcentrationoffluorescentdyesinelectromembranesystemsviaelectrophoreticmigration |