Effects of bile acid sequestration on hepatic steatosis in obese mice
Background. Bile acid sequestration (BAS) with resins has shown antidiabetic effects in both humans and animals. Since hepatic steatosis is commonly associated with type 2 diabetes mellitus and the effects of BAS on steatosis have not been explored in detail, we evaluated the effects of cholestyrami...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119309111 |
| _version_ | 1818740646255525888 |
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| author | Nancy Solís Margarita Pizarro Pablo Quintero Juan Pablo Arab Arnoldo Riquelme Oslando Padilla Gonzalo Carrasco Carlos J. Pirola Silvia Sookoian Marco Arrese |
| author_facet | Nancy Solís Margarita Pizarro Pablo Quintero Juan Pablo Arab Arnoldo Riquelme Oslando Padilla Gonzalo Carrasco Carlos J. Pirola Silvia Sookoian Marco Arrese |
| author_sort | Nancy Solís |
| collection | DOAJ |
| description | Background. Bile acid sequestration (BAS) with resins has shown antidiabetic effects in both humans and animals. Since hepatic steatosis is commonly associated with type 2 diabetes mellitus and the effects of BAS on steatosis have not been explored in detail, we evaluated the effects of cholestyramine (CTM) administration on fatty liver development in the leptin-deficient obese mice.Aim. To study the effects of BAS on fatty liver development in obese (ob/ob) mice.Material and methods. 4 week-old ob/ob mice (B6.V-Le-pob/J, n = 4-6 per group) were fed with or without CTM (control group) during 8 weeks. Serum and biliary parameters, glucose tolerance test (GTT), hepatic triglyceride content, liver histology and hepatic gene expression of relevant genes related to bile secretion, lipid and glucose metabolism were assessed.Results. Control 12-week-old mice exhibited marked obesity and hepatic steatosis. CTM administration expectedly determined a marked de-repression of 7-α-hydroxylase and decreased biliary bile acid secretion as well as improved GTT. CTM feeding showed no effects on hepatic triglyceride content or in the degree of steatosis on liver histology. CTM was associated with increased levels of serum alanine-aminotransferase.Conclusion. Although CTM administration positively affects glucose tolerance it does not prevent hepatic steatosis development in obese mice. Moreover, CTM feeding was associated to liver enzyme elevation in this model of NAFLD. Thus, the effects BAS on NAFLD need to be specifically addressed since this therapy might not be beneficial for this condition. |
| first_indexed | 2024-12-18T01:44:02Z |
| format | Article |
| id | doaj.art-2e355d80233f448dba3e6f1bd7dd108a |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-18T01:44:02Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-2e355d80233f448dba3e6f1bd7dd108a2022-12-21T21:25:13ZengElsevierAnnals of Hepatology1665-26812014-01-01131105112Effects of bile acid sequestration on hepatic steatosis in obese miceNancy Solís0Margarita Pizarro1Pablo Quintero2Juan Pablo Arab3Arnoldo Riquelme4Oslando Padilla5Gonzalo Carrasco6Carlos J. Pirola7Silvia Sookoian8Marco Arrese9Departamento de Gastroenterología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Gastroenterología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Gastroenterología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Gastroenterología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Gastroenterología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Salud Pública, Pontificia Universidad Católica de Chile, Santiago, Chile.Hospital Parroquial de San Bernardo, San Bernardo, ChileInstitute of Medical Research (IDIM), University of Buenos Aires, National Council of Scientific and Technological Research (CONICET), Autonomous City of Buenos Aires 1427, ArgentinaInstitute of Medical Research (IDIM), University of Buenos Aires, National Council of Scientific and Technological Research (CONICET), Autonomous City of Buenos Aires 1427, ArgentinaDepartamento de Gastroenterología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile; Correspondence and reprint request:Background. Bile acid sequestration (BAS) with resins has shown antidiabetic effects in both humans and animals. Since hepatic steatosis is commonly associated with type 2 diabetes mellitus and the effects of BAS on steatosis have not been explored in detail, we evaluated the effects of cholestyramine (CTM) administration on fatty liver development in the leptin-deficient obese mice.Aim. To study the effects of BAS on fatty liver development in obese (ob/ob) mice.Material and methods. 4 week-old ob/ob mice (B6.V-Le-pob/J, n = 4-6 per group) were fed with or without CTM (control group) during 8 weeks. Serum and biliary parameters, glucose tolerance test (GTT), hepatic triglyceride content, liver histology and hepatic gene expression of relevant genes related to bile secretion, lipid and glucose metabolism were assessed.Results. Control 12-week-old mice exhibited marked obesity and hepatic steatosis. CTM administration expectedly determined a marked de-repression of 7-α-hydroxylase and decreased biliary bile acid secretion as well as improved GTT. CTM feeding showed no effects on hepatic triglyceride content or in the degree of steatosis on liver histology. CTM was associated with increased levels of serum alanine-aminotransferase.Conclusion. Although CTM administration positively affects glucose tolerance it does not prevent hepatic steatosis development in obese mice. Moreover, CTM feeding was associated to liver enzyme elevation in this model of NAFLD. Thus, the effects BAS on NAFLD need to be specifically addressed since this therapy might not be beneficial for this condition.http://www.sciencedirect.com/science/article/pii/S1665268119309111NAFLDObese miceBile acidsSteatosisFatty liverCholestyramine |
| spellingShingle | Nancy Solís Margarita Pizarro Pablo Quintero Juan Pablo Arab Arnoldo Riquelme Oslando Padilla Gonzalo Carrasco Carlos J. Pirola Silvia Sookoian Marco Arrese Effects of bile acid sequestration on hepatic steatosis in obese mice Annals of Hepatology NAFLD Obese mice Bile acids Steatosis Fatty liver Cholestyramine |
| title | Effects of bile acid sequestration on hepatic steatosis in obese mice |
| title_full | Effects of bile acid sequestration on hepatic steatosis in obese mice |
| title_fullStr | Effects of bile acid sequestration on hepatic steatosis in obese mice |
| title_full_unstemmed | Effects of bile acid sequestration on hepatic steatosis in obese mice |
| title_short | Effects of bile acid sequestration on hepatic steatosis in obese mice |
| title_sort | effects of bile acid sequestration on hepatic steatosis in obese mice |
| topic | NAFLD Obese mice Bile acids Steatosis Fatty liver Cholestyramine |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119309111 |
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