Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase
Objective: Leptin is a cytokine produced by adipose tissue that acts mainly on the hypothalamus to regulate appetite and energy homeostasis. Previous studies revealed that the leptin receptor is expressed not only in neurons, but also in glial cells. Microglia are resident immune cells in the brain...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Molecular Metabolism |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212877817307391 |
| _version_ | 1818035737138823168 |
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| author | Yuanqing Gao Andrés Vidal-Itriago Irina Milanova Nikita L. Korpel Martin J. Kalsbeek Robby Zachariah Tom Andries Kalsbeek Susanna M. Hofmann Chun-Xia Yi |
| author_facet | Yuanqing Gao Andrés Vidal-Itriago Irina Milanova Nikita L. Korpel Martin J. Kalsbeek Robby Zachariah Tom Andries Kalsbeek Susanna M. Hofmann Chun-Xia Yi |
| author_sort | Yuanqing Gao |
| collection | DOAJ |
| description | Objective: Leptin is a cytokine produced by adipose tissue that acts mainly on the hypothalamus to regulate appetite and energy homeostasis. Previous studies revealed that the leptin receptor is expressed not only in neurons, but also in glial cells. Microglia are resident immune cells in the brain that play an essential role in immune defense and neural network development. Previously we reported that microglial morphology and cytokine production are changed in the leptin receptor deficient db/db mouse, suggesting that leptin's central effects on metabolic control might involve signaling through microglia. In the current study, we aimed to uncover the role of leptin signaling in microglia in systemic metabolic control. Methods: We generated a mouse model with leptin receptor deficiency, specifically in the myeloid cells, to determine the role of microglial leptin signaling in the development of metabolic disease and to investigate microglial functions. Results: We discovered that these mice have increased body weight with hyperphagia. In the hypothalamus, pro-opiomelanocortin neuron numbers in the arcuate nucleus (ARC) and α-MSH projections from the ARC to the paraventricular nucleus (PVN) decreased, which was accompanied by the presence of less ramified microglia with impaired phagocytic capacity in the PVN. Conclusions: Myeloid cell leptin receptor deficient mice partially replicate the db/db phenotype. Leptin signaling in hypothalamic microglia is important for microglial function and a correct formation of the hypothalamic neuronal circuit regulating metabolism. Author Video: Author Video Watch what authors say about their articles Keywords: Microglia, Diabetes, Obesity, POMC, α-MSH |
| first_indexed | 2024-12-10T06:59:48Z |
| format | Article |
| id | doaj.art-2e394c22479d46e49007fac6411a842f |
| institution | Directory Open Access Journal |
| issn | 2212-8778 |
| language | English |
| last_indexed | 2024-12-10T06:59:48Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj.art-2e394c22479d46e49007fac6411a842f2022-12-22T01:58:21ZengElsevierMolecular Metabolism2212-87782018-01-017155160Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increaseYuanqing Gao0Andrés Vidal-Itriago1Irina Milanova2Nikita L. Korpel3Martin J. Kalsbeek4Robby Zachariah Tom5Andries Kalsbeek6Susanna M. Hofmann7Chun-Xia Yi8Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The NetherlandsDepartment of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The NetherlandsDepartment of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The NetherlandsDepartment of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The NetherlandsDepartment of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The NetherlandsInstitute for Diabetes and Regeneration Research & Helmholtz Diabetes Center, Helmholtz Zentrum München, GermanyDepartment of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The Netherlands; Hypothalamic Integration Mechanisms, Netherlands Institute for Neuroscience, Amsterdam, The NetherlandsInstitute for Diabetes and Regeneration Research & Helmholtz Diabetes Center, Helmholtz Zentrum München, Germany; German Center for Diabetes Research (DZD), Neuherberg, Germany; Medizinische Klinik und Poliklinik IV, Klinikum der LMU, München, GermanyDepartment of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The Netherlands; Corresponding author. Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands. Fax: +31 20 6917682.Objective: Leptin is a cytokine produced by adipose tissue that acts mainly on the hypothalamus to regulate appetite and energy homeostasis. Previous studies revealed that the leptin receptor is expressed not only in neurons, but also in glial cells. Microglia are resident immune cells in the brain that play an essential role in immune defense and neural network development. Previously we reported that microglial morphology and cytokine production are changed in the leptin receptor deficient db/db mouse, suggesting that leptin's central effects on metabolic control might involve signaling through microglia. In the current study, we aimed to uncover the role of leptin signaling in microglia in systemic metabolic control. Methods: We generated a mouse model with leptin receptor deficiency, specifically in the myeloid cells, to determine the role of microglial leptin signaling in the development of metabolic disease and to investigate microglial functions. Results: We discovered that these mice have increased body weight with hyperphagia. In the hypothalamus, pro-opiomelanocortin neuron numbers in the arcuate nucleus (ARC) and α-MSH projections from the ARC to the paraventricular nucleus (PVN) decreased, which was accompanied by the presence of less ramified microglia with impaired phagocytic capacity in the PVN. Conclusions: Myeloid cell leptin receptor deficient mice partially replicate the db/db phenotype. Leptin signaling in hypothalamic microglia is important for microglial function and a correct formation of the hypothalamic neuronal circuit regulating metabolism. Author Video: Author Video Watch what authors say about their articles Keywords: Microglia, Diabetes, Obesity, POMC, α-MSHhttp://www.sciencedirect.com/science/article/pii/S2212877817307391 |
| spellingShingle | Yuanqing Gao Andrés Vidal-Itriago Irina Milanova Nikita L. Korpel Martin J. Kalsbeek Robby Zachariah Tom Andries Kalsbeek Susanna M. Hofmann Chun-Xia Yi Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase Molecular Metabolism |
| title | Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase |
| title_full | Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase |
| title_fullStr | Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase |
| title_full_unstemmed | Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase |
| title_short | Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase |
| title_sort | deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase |
| url | http://www.sciencedirect.com/science/article/pii/S2212877817307391 |
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