Nitrite Biosensing via Selective Enzymes—A Long but Promising Route
The last decades have witnessed a steady increase of the social and political awareness for the need of monitoring and controlling environmental and industrial processes. In the case of nitrite ion, due to its potential toxicity for human health, the European Union has recently implemented a number...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-12-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/10/12/11530/ |
| _version_ | 1811184698708197376 |
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| author | M. Gabriela Almeida Alexandra Serra Celia M. Silveira Jose J.G. Moura |
| author_facet | M. Gabriela Almeida Alexandra Serra Celia M. Silveira Jose J.G. Moura |
| author_sort | M. Gabriela Almeida |
| collection | DOAJ |
| description | The last decades have witnessed a steady increase of the social and political awareness for the need of monitoring and controlling environmental and industrial processes. In the case of nitrite ion, due to its potential toxicity for human health, the European Union has recently implemented a number of rules to restrict its level in drinking waters and food products. Although several analytical protocols have been proposed for nitrite quantification, none of them enable a reliable and quick analysis of complex samples. An alternative approach relies on the construction of biosensing devices using stable enzymes, with both high activity and specificity for nitrite. In this paper we review the current state-of-the-art in the field of electrochemical and optical biosensors using nitrite reducing enzymes as biorecognition elements and discuss the opportunities and challenges in this emerging market. |
| first_indexed | 2024-04-11T13:16:50Z |
| format | Article |
| id | doaj.art-2e395cadbba54520b63d519c054696b4 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:16:50Z |
| publishDate | 2010-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-2e395cadbba54520b63d519c054696b42022-12-22T04:22:21ZengMDPI AGSensors1424-82202010-12-011012115301155510.3390/s101211530Nitrite Biosensing via Selective Enzymes—A Long but Promising RouteM. Gabriela AlmeidaAlexandra SerraCelia M. SilveiraJose J.G. MouraThe last decades have witnessed a steady increase of the social and political awareness for the need of monitoring and controlling environmental and industrial processes. In the case of nitrite ion, due to its potential toxicity for human health, the European Union has recently implemented a number of rules to restrict its level in drinking waters and food products. Although several analytical protocols have been proposed for nitrite quantification, none of them enable a reliable and quick analysis of complex samples. An alternative approach relies on the construction of biosensing devices using stable enzymes, with both high activity and specificity for nitrite. In this paper we review the current state-of-the-art in the field of electrochemical and optical biosensors using nitrite reducing enzymes as biorecognition elements and discuss the opportunities and challenges in this emerging market.http://www.mdpi.com/1424-8220/10/12/11530/nitrite, biosensorsnitrite reductaseselectrochemical transducers |
| spellingShingle | M. Gabriela Almeida Alexandra Serra Celia M. Silveira Jose J.G. Moura Nitrite Biosensing via Selective Enzymes—A Long but Promising Route Sensors nitrite, biosensors nitrite reductases electrochemical transducers |
| title | Nitrite Biosensing via Selective Enzymes—A Long but Promising Route |
| title_full | Nitrite Biosensing via Selective Enzymes—A Long but Promising Route |
| title_fullStr | Nitrite Biosensing via Selective Enzymes—A Long but Promising Route |
| title_full_unstemmed | Nitrite Biosensing via Selective Enzymes—A Long but Promising Route |
| title_short | Nitrite Biosensing via Selective Enzymes—A Long but Promising Route |
| title_sort | nitrite biosensing via selective enzymes a long but promising route |
| topic | nitrite, biosensors nitrite reductases electrochemical transducers |
| url | http://www.mdpi.com/1424-8220/10/12/11530/ |
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