Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types
Antibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect pathogens in field samples, especially in hard-to-reach remote areas that may have limited access to central laboratories during an outbreak or surveillance. In this study, we investigated the ability of a commerc...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2076-0817/11/2/138 |
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| author | Chukwunonso Onyilagha Kelvin Nguyen Pam D. Luka Ularamu Hussaini Adeyinka Adedeji Theophilus Odoom Aruna Ambagala |
| author_facet | Chukwunonso Onyilagha Kelvin Nguyen Pam D. Luka Ularamu Hussaini Adeyinka Adedeji Theophilus Odoom Aruna Ambagala |
| author_sort | Chukwunonso Onyilagha |
| collection | DOAJ |
| description | Antibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect pathogens in field samples, especially in hard-to-reach remote areas that may have limited access to central laboratories during an outbreak or surveillance. In this study, we investigated the ability of a commercially available LFA, PenCheck<sup>®</sup>, to detect African swine fever virus (ASFV) in clinical samples derived from pigs infected with highly virulent ASFV strains. The assay was specific and positively identified the majority of pigs showing high fever during the early stages (between 3 and 5 days) of infection. PenCheck<sup>®</sup> LFA also detected ASFV in serum and tissue samples collected from pigs that succumbed to experimental ASFV infection and whole blood, plasma, and tissue samples from the field. The limit of detection of the assay was ASFV titer 10<sup>7.80</sup> TCID<sub>50</sub>/mL, corresponding to ASFV real-time PCR values below 23 Ct. Although the sensitivity of the assay is less than that of the laboratory-based real-time PCR assays, the results obtained with the PenCheck<sup>®</sup> LFA in this study suggest that it can be used as a herd-level, field-deployable, and easy-to-use diagnostic tool to identify ASF-affected farms when access to portable molecular assays or central laboratories is not possible. |
| first_indexed | 2024-03-09T21:16:42Z |
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| id | doaj.art-2e3b7f615493450a90c7edd4f7bd9919 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T21:16:42Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-2e3b7f615493450a90c7edd4f7bd99192023-11-23T21:30:59ZengMDPI AGPathogens2076-08172022-01-0111213810.3390/pathogens11020138Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample TypesChukwunonso Onyilagha0Kelvin Nguyen1Pam D. Luka2Ularamu Hussaini3Adeyinka Adedeji4Theophilus Odoom5Aruna Ambagala6National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, CanadaVirology Division, National Veterinary Research Institute, P.M.B 01, Vom 930001, Plateau, NigeriaVirology Division, National Veterinary Research Institute, P.M.B 01, Vom 930001, Plateau, NigeriaVirology Division, National Veterinary Research Institute, P.M.B 01, Vom 930001, Plateau, NigeriaAccra Laboratory, Veterinary Services Directorate, Accra P.O. Box M161, GhanaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, CanadaAntibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect pathogens in field samples, especially in hard-to-reach remote areas that may have limited access to central laboratories during an outbreak or surveillance. In this study, we investigated the ability of a commercially available LFA, PenCheck<sup>®</sup>, to detect African swine fever virus (ASFV) in clinical samples derived from pigs infected with highly virulent ASFV strains. The assay was specific and positively identified the majority of pigs showing high fever during the early stages (between 3 and 5 days) of infection. PenCheck<sup>®</sup> LFA also detected ASFV in serum and tissue samples collected from pigs that succumbed to experimental ASFV infection and whole blood, plasma, and tissue samples from the field. The limit of detection of the assay was ASFV titer 10<sup>7.80</sup> TCID<sub>50</sub>/mL, corresponding to ASFV real-time PCR values below 23 Ct. Although the sensitivity of the assay is less than that of the laboratory-based real-time PCR assays, the results obtained with the PenCheck<sup>®</sup> LFA in this study suggest that it can be used as a herd-level, field-deployable, and easy-to-use diagnostic tool to identify ASF-affected farms when access to portable molecular assays or central laboratories is not possible.https://www.mdpi.com/2076-0817/11/2/138African swine feverlateral flow assayPenCheckreal-time PCR |
| spellingShingle | Chukwunonso Onyilagha Kelvin Nguyen Pam D. Luka Ularamu Hussaini Adeyinka Adedeji Theophilus Odoom Aruna Ambagala Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types Pathogens African swine fever lateral flow assay PenCheck real-time PCR |
| title | Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types |
| title_full | Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types |
| title_fullStr | Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types |
| title_full_unstemmed | Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types |
| title_short | Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types |
| title_sort | evaluation of a lateral flow assay for rapid detection of african swine fever virus in multiple sample types |
| topic | African swine fever lateral flow assay PenCheck real-time PCR |
| url | https://www.mdpi.com/2076-0817/11/2/138 |
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