Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China
Abstract Background Pigeon circovirus (PiCV) infections in pigeons (Columba livia) have been reported worldwide. Currently, pigeon racing is becoming increasingly popular and considered to be a national sport in China, and even, the greatest competitions of racing pigeons are taking place in China....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-022-08425-8 |
| _version_ | 1818050976222806016 |
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| author | Haoran Wang Hui Gao Zhiwen Jiang Leibo Shi Pengwei Zhao Yanming Zhang Chengbao Wang |
| author_facet | Haoran Wang Hui Gao Zhiwen Jiang Leibo Shi Pengwei Zhao Yanming Zhang Chengbao Wang |
| author_sort | Haoran Wang |
| collection | DOAJ |
| description | Abstract Background Pigeon circovirus (PiCV) infections in pigeons (Columba livia) have been reported worldwide. Currently, pigeon racing is becoming increasingly popular and considered to be a national sport in China, and even, the greatest competitions of racing pigeons are taking place in China. However, there are still no epidemiologic data regarding PiCV infections among racing pigeons in China. The purpose of our study was to provide information of prevalence, genetic variation and evolution of PiCV from racing pigeons in China. Results To trace the prevalence, genetic variation and evolution of PiCV in sick and healthy racing pigeons, 622 samples were collected from 11 provinces or municipalities in China from 2016 to 2019. The results showed that the positive rate of PiCV was 19.3% (120/622) at the sample level and 59.0% (23/39) at the club level, thus suggesting that the virus was prevalent in Chinese racing pigeons. A sequence analysis revealed that the cap genes of the PiCV strains identified in our study displayed a high genetic diversity and shared nucleotide homologies of 71.9%–100% and amino acid homologies of 71.7%–100%. 28 and 36 unique amino acid substitutions were observed in the Cap and Rep proteins derived from our PiCV strains, respectively. A cladogram representation of PiCV strains phylogeny based on 90 cap gene sequences showed that the strains in this study could be further divided into seven clades (A, B, C, E, G, H, and I) and some of them were closely related to worldwide strains from different types of pigeons. A large number of recombination events (31 events) were also detected in the PiCV genomes from Chinese racing pigeons. Conclusions These findings indicate that PiCV strains circulating in China exhibit a high genetic diversity and also contribute to information of prevalence, genetic variation and evolution of PiCV from racing pigeons in China. |
| first_indexed | 2024-12-10T11:02:01Z |
| format | Article |
| id | doaj.art-2e3c2059664444e5b8bedafd4aa802ce |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-10T11:02:01Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-2e3c2059664444e5b8bedafd4aa802ce2022-12-22T01:51:39ZengBMCBMC Genomics1471-21642022-04-0123111210.1186/s12864-022-08425-8Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern ChinaHaoran Wang0Hui Gao1Zhiwen Jiang2Leibo Shi3Pengwei Zhao4Yanming Zhang5Chengbao Wang6College of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityAbstract Background Pigeon circovirus (PiCV) infections in pigeons (Columba livia) have been reported worldwide. Currently, pigeon racing is becoming increasingly popular and considered to be a national sport in China, and even, the greatest competitions of racing pigeons are taking place in China. However, there are still no epidemiologic data regarding PiCV infections among racing pigeons in China. The purpose of our study was to provide information of prevalence, genetic variation and evolution of PiCV from racing pigeons in China. Results To trace the prevalence, genetic variation and evolution of PiCV in sick and healthy racing pigeons, 622 samples were collected from 11 provinces or municipalities in China from 2016 to 2019. The results showed that the positive rate of PiCV was 19.3% (120/622) at the sample level and 59.0% (23/39) at the club level, thus suggesting that the virus was prevalent in Chinese racing pigeons. A sequence analysis revealed that the cap genes of the PiCV strains identified in our study displayed a high genetic diversity and shared nucleotide homologies of 71.9%–100% and amino acid homologies of 71.7%–100%. 28 and 36 unique amino acid substitutions were observed in the Cap and Rep proteins derived from our PiCV strains, respectively. A cladogram representation of PiCV strains phylogeny based on 90 cap gene sequences showed that the strains in this study could be further divided into seven clades (A, B, C, E, G, H, and I) and some of them were closely related to worldwide strains from different types of pigeons. A large number of recombination events (31 events) were also detected in the PiCV genomes from Chinese racing pigeons. Conclusions These findings indicate that PiCV strains circulating in China exhibit a high genetic diversity and also contribute to information of prevalence, genetic variation and evolution of PiCV from racing pigeons in China.https://doi.org/10.1186/s12864-022-08425-8Genetic diversityMolecular epidemiologyPhylogenetic analysisPigeon circovirusSequence analysis |
| spellingShingle | Haoran Wang Hui Gao Zhiwen Jiang Leibo Shi Pengwei Zhao Yanming Zhang Chengbao Wang Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China BMC Genomics Genetic diversity Molecular epidemiology Phylogenetic analysis Pigeon circovirus Sequence analysis |
| title | Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China |
| title_full | Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China |
| title_fullStr | Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China |
| title_full_unstemmed | Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China |
| title_short | Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China |
| title_sort | molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in northern china |
| topic | Genetic diversity Molecular epidemiology Phylogenetic analysis Pigeon circovirus Sequence analysis |
| url | https://doi.org/10.1186/s12864-022-08425-8 |
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