Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal
The development of efficient strategies for wastewater treatment to remove micropollutants is of the highest importance. Hence, in this study, we presented a rapid approach to the production of biocatalytic membranes based on commercially available cellulose membrane and oxidoreductase enzymes inclu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/14086 |
| _version_ | 1797465088628621312 |
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| author | Agata Zdarta Jakub Zdarta |
| author_facet | Agata Zdarta Jakub Zdarta |
| author_sort | Agata Zdarta |
| collection | DOAJ |
| description | The development of efficient strategies for wastewater treatment to remove micropollutants is of the highest importance. Hence, in this study, we presented a rapid approach to the production of biocatalytic membranes based on commercially available cellulose membrane and oxidoreductase enzymes including laccase, tyrosinase, and horseradish peroxidase. Effective enzyme deposition was confirmed based on Fourier transform infrared spectra, whereas results of spectrophotometric measurements showed that immobilization yield for all proposed systems exceeded 80% followed by over 80% activity recovery, with the highest values (over 90%) noticed for the membrane-laccase system. Further, storage stability and reusability of the immobilized enzyme were improved, reaching over 75% after, respectively, 20 days of storage, and 10 repeated biocatalytic cycles. The key stage of the study concerned the use of produced membranes for the removal of hematoporphyrin, (2,4-dichlorophenoxy)acetic acid (2,4-D), 17α-ethynylestradiol, tetracycline, tert-amyl alcohol (anesthetic drug), and ketoprofen methyl ester from real wastewater sampling at various places in the wastewater treatment plant. Although produced membranes showed mixed removal rates, all of the analyzed compounds were at least partially removed from the wastewater. Obtained data clearly showed, however, that composition of the wastewater matrix, type of pollutants as well as type of enzyme strongly affect the efficiency of enzymatic treatment of wastewater. |
| first_indexed | 2024-03-09T18:16:27Z |
| format | Article |
| id | doaj.art-2e4124910e954578a61b6bce31a7e4c7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:16:27Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2e4124910e954578a61b6bce31a7e4c72023-11-24T08:38:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221408610.3390/ijms232214086Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants RemovalAgata Zdarta0Jakub Zdarta1Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60695 Poznan, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60695 Poznan, PolandThe development of efficient strategies for wastewater treatment to remove micropollutants is of the highest importance. Hence, in this study, we presented a rapid approach to the production of biocatalytic membranes based on commercially available cellulose membrane and oxidoreductase enzymes including laccase, tyrosinase, and horseradish peroxidase. Effective enzyme deposition was confirmed based on Fourier transform infrared spectra, whereas results of spectrophotometric measurements showed that immobilization yield for all proposed systems exceeded 80% followed by over 80% activity recovery, with the highest values (over 90%) noticed for the membrane-laccase system. Further, storage stability and reusability of the immobilized enzyme were improved, reaching over 75% after, respectively, 20 days of storage, and 10 repeated biocatalytic cycles. The key stage of the study concerned the use of produced membranes for the removal of hematoporphyrin, (2,4-dichlorophenoxy)acetic acid (2,4-D), 17α-ethynylestradiol, tetracycline, tert-amyl alcohol (anesthetic drug), and ketoprofen methyl ester from real wastewater sampling at various places in the wastewater treatment plant. Although produced membranes showed mixed removal rates, all of the analyzed compounds were at least partially removed from the wastewater. Obtained data clearly showed, however, that composition of the wastewater matrix, type of pollutants as well as type of enzyme strongly affect the efficiency of enzymatic treatment of wastewater.https://www.mdpi.com/1422-0067/23/22/14086enzyme immobilizationoxidoreductasemembranesenzymatic membrane reactorswastewater treatmentbioremoval |
| spellingShingle | Agata Zdarta Jakub Zdarta Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal International Journal of Molecular Sciences enzyme immobilization oxidoreductase membranes enzymatic membrane reactors wastewater treatment bioremoval |
| title | Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal |
| title_full | Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal |
| title_fullStr | Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal |
| title_full_unstemmed | Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal |
| title_short | Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal |
| title_sort | study of membrane immobilized oxidoreductases in wastewater treatment for micropollutants removal |
| topic | enzyme immobilization oxidoreductase membranes enzymatic membrane reactors wastewater treatment bioremoval |
| url | https://www.mdpi.com/1422-0067/23/22/14086 |
| work_keys_str_mv | AT agatazdarta studyofmembraneimmobilizedoxidoreductasesinwastewatertreatmentformicropollutantsremoval AT jakubzdarta studyofmembraneimmobilizedoxidoreductasesinwastewatertreatmentformicropollutantsremoval |