Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi Sheep
Several genes influence sheep’s reproductive performance, among them the paired-like homeodomain transcription factor 2 ( PITX2 ) gene. Thus, this study aimed to examine whether the variability within the PITX2 gene is associated with the reproductive performance of Awassi ewes. A total of 123 singl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2023-06-01
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| Series: | Bioinformatics and Biology Insights |
| Online Access: | https://doi.org/10.1177/11779322231179018 |
| _version_ | 1797810241394442240 |
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| author | Ahmed H Alkhammas Tahreer M Al-Thuwaini Mohammed Baqur S Al-Shuhaib Neam M Khazaal |
| author_facet | Ahmed H Alkhammas Tahreer M Al-Thuwaini Mohammed Baqur S Al-Shuhaib Neam M Khazaal |
| author_sort | Ahmed H Alkhammas |
| collection | DOAJ |
| description | Several genes influence sheep’s reproductive performance, among them the paired-like homeodomain transcription factor 2 ( PITX2 ) gene. Thus, this study aimed to examine whether the variability within the PITX2 gene is associated with the reproductive performance of Awassi ewes. A total of 123 single-progeny ewes and 109 twin ewes were used to extract genomic DNA. An amplicon of 4 sequence fragments from exons 2, 4, 5 (upstream portion), and 5 (downstream portion) of the PITX2 gene was generated by polymerase chain reaction (PCR), 228, 304, 381, and 382 bp, respectively. Three genotypes of 382 bp amplicons were identified: CC, CT, and TT. Sequence analysis revealed a novel mutation in the CT genotype 319C > T. Statistical analysis revealed that single-nucleotide polymorphism (SNP) 319C > T was associated with reproductive performance. Single-nucleotide polymorphism 319C > T-carrying ewes had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing than those carrying CT and CC genotypes. Based on a logistic regression analysis, it was confirmed that the 319C > T SNP decreased litter size. Ewes with TT genotype produced fewer lambs than ewes with CT and CC genotypes. According to these results, the variant 319C> T SNP negatively affects the reproductive performance of Awassi sheep. Ewes carrying the 319C > T SNP have a lower litter size and are less prolific than those without the SNP. |
| first_indexed | 2024-03-13T07:06:19Z |
| format | Article |
| id | doaj.art-2e4274772dd84855bb11e563bb627b93 |
| institution | Directory Open Access Journal |
| issn | 1177-9322 |
| language | English |
| last_indexed | 2024-03-13T07:06:19Z |
| publishDate | 2023-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Bioinformatics and Biology Insights |
| spelling | doaj.art-2e4274772dd84855bb11e563bb627b932023-06-06T09:33:29ZengSAGE PublishingBioinformatics and Biology Insights1177-93222023-06-011710.1177/11779322231179018Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi SheepAhmed H Alkhammas0Tahreer M Al-Thuwaini1Mohammed Baqur S Al-Shuhaib2Neam M Khazaal3Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqSeveral genes influence sheep’s reproductive performance, among them the paired-like homeodomain transcription factor 2 ( PITX2 ) gene. Thus, this study aimed to examine whether the variability within the PITX2 gene is associated with the reproductive performance of Awassi ewes. A total of 123 single-progeny ewes and 109 twin ewes were used to extract genomic DNA. An amplicon of 4 sequence fragments from exons 2, 4, 5 (upstream portion), and 5 (downstream portion) of the PITX2 gene was generated by polymerase chain reaction (PCR), 228, 304, 381, and 382 bp, respectively. Three genotypes of 382 bp amplicons were identified: CC, CT, and TT. Sequence analysis revealed a novel mutation in the CT genotype 319C > T. Statistical analysis revealed that single-nucleotide polymorphism (SNP) 319C > T was associated with reproductive performance. Single-nucleotide polymorphism 319C > T-carrying ewes had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing than those carrying CT and CC genotypes. Based on a logistic regression analysis, it was confirmed that the 319C > T SNP decreased litter size. Ewes with TT genotype produced fewer lambs than ewes with CT and CC genotypes. According to these results, the variant 319C> T SNP negatively affects the reproductive performance of Awassi sheep. Ewes carrying the 319C > T SNP have a lower litter size and are less prolific than those without the SNP.https://doi.org/10.1177/11779322231179018 |
| spellingShingle | Ahmed H Alkhammas Tahreer M Al-Thuwaini Mohammed Baqur S Al-Shuhaib Neam M Khazaal Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi Sheep Bioinformatics and Biology Insights |
| title | Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi Sheep |
| title_full | Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi Sheep |
| title_fullStr | Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi Sheep |
| title_full_unstemmed | Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi Sheep |
| title_short | Association of Novel C319T Variant of Gene 3’UTR Region With Reproductive Performance in Awassi Sheep |
| title_sort | association of novel c319t variant of gene 3 utr region with reproductive performance in awassi sheep |
| url | https://doi.org/10.1177/11779322231179018 |
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