Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2
Abstract The shortage of molecular information for taxol-producing fungi has greatly impeded the understanding of fungal taxol biosynthesis mechanism. In this study, the transcriptome of one taxol-producing endophytic fungus Cladosporium cladosporioides MD2 was sequenced for the first time. About 1....
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-03-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0567-6 |
| _version_ | 1818338095993454592 |
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| author | Li-Yun Miao Xin-Chun Mo Xiao-Yuan Xi Lan Zhou Ge De You-Sheng Ke Pan Liu Fa-Jun Song Wen-Wen Jin Peng Zhang |
| author_facet | Li-Yun Miao Xin-Chun Mo Xiao-Yuan Xi Lan Zhou Ge De You-Sheng Ke Pan Liu Fa-Jun Song Wen-Wen Jin Peng Zhang |
| author_sort | Li-Yun Miao |
| collection | DOAJ |
| description | Abstract The shortage of molecular information for taxol-producing fungi has greatly impeded the understanding of fungal taxol biosynthesis mechanism. In this study, the transcriptome of one taxol-producing endophytic fungus Cladosporium cladosporioides MD2 was sequenced for the first time. About 1.77 Gbp clean reads were generated and further assembled into 16,603 unigenes with an average length of 1110 bp. All of the unigenes were annotated against seven public databases to present the transcriptome characteristics of C. cladosporioides MD2. A total of 12,479 unigenes could be annotated with at least one database, and 1593 unigenes could be annotated in all queried databases. In total, 8425 and 3350 unigenes were categorized into 57 GO functional groups and 262 KEGG pathways, respectively, exhibiting the dominant GO terms and metabolic pathways in the C. cladosporioides MD2 transcriptome. One potential and partial taxol biosynthetic pathway was speculated including 9 unigenes related to terpenoid backbone biosynthesis and 40 unigenes involved in the biosynthetic steps from geranylgeranyl diphosphate to 10-deacetylbaccatin III. These results provided valuable information for the molecular mechanism research of taxol biosynthesis in C. cladosporioides MD2. |
| first_indexed | 2024-12-13T15:05:40Z |
| format | Article |
| id | doaj.art-2e4341cff26047939f3c5eea450dae20 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T15:05:40Z |
| publishDate | 2018-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-2e4341cff26047939f3c5eea450dae202022-12-21T23:41:01ZengSpringerOpenAMB Express2191-08552018-03-018111010.1186/s13568-018-0567-6Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2Li-Yun Miao0Xin-Chun Mo1Xiao-Yuan Xi2Lan Zhou3Ge De4You-Sheng Ke5Pan Liu6Fa-Jun Song7Wen-Wen Jin8Peng Zhang9Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesSchool of Applied Technology, Lijiang Teacher CollegeHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesSchool of Life Science and Technology, Huazhong University of Science and TechnologyHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for NationalitiesAbstract The shortage of molecular information for taxol-producing fungi has greatly impeded the understanding of fungal taxol biosynthesis mechanism. In this study, the transcriptome of one taxol-producing endophytic fungus Cladosporium cladosporioides MD2 was sequenced for the first time. About 1.77 Gbp clean reads were generated and further assembled into 16,603 unigenes with an average length of 1110 bp. All of the unigenes were annotated against seven public databases to present the transcriptome characteristics of C. cladosporioides MD2. A total of 12,479 unigenes could be annotated with at least one database, and 1593 unigenes could be annotated in all queried databases. In total, 8425 and 3350 unigenes were categorized into 57 GO functional groups and 262 KEGG pathways, respectively, exhibiting the dominant GO terms and metabolic pathways in the C. cladosporioides MD2 transcriptome. One potential and partial taxol biosynthetic pathway was speculated including 9 unigenes related to terpenoid backbone biosynthesis and 40 unigenes involved in the biosynthetic steps from geranylgeranyl diphosphate to 10-deacetylbaccatin III. These results provided valuable information for the molecular mechanism research of taxol biosynthesis in C. cladosporioides MD2.http://link.springer.com/article/10.1186/s13568-018-0567-6Endophytic fungusCladosporium cladosporioidesTranscriptomeTaxol |
| spellingShingle | Li-Yun Miao Xin-Chun Mo Xiao-Yuan Xi Lan Zhou Ge De You-Sheng Ke Pan Liu Fa-Jun Song Wen-Wen Jin Peng Zhang Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2 AMB Express Endophytic fungus Cladosporium cladosporioides Transcriptome Taxol |
| title | Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2 |
| title_full | Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2 |
| title_fullStr | Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2 |
| title_full_unstemmed | Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2 |
| title_short | Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2 |
| title_sort | transcriptome analysis of a taxol producing endophytic fungus cladosporium cladosporioides md2 |
| topic | Endophytic fungus Cladosporium cladosporioides Transcriptome Taxol |
| url | http://link.springer.com/article/10.1186/s13568-018-0567-6 |
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