Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and Cosmetology
Due to the growing popularity of herbal extract-loaded hydrogels, this study assessed the biological activity of extracts and hydrogels containing three types (water (WE), water–ethanol (EE) and water–glycerin (GE)) of <i>Cornus mas</i> L. (dogwood) extracts. The content of biologically...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/21/7384 |
| _version_ | 1827765545235120128 |
|---|---|
| author | Martyna Zagórska-Dziok Aleksandra Ziemlewska Agnieszka Mokrzyńska Zofia Nizioł-Łukaszewska Magdalena Wójciak Ireneusz Sowa |
| author_facet | Martyna Zagórska-Dziok Aleksandra Ziemlewska Agnieszka Mokrzyńska Zofia Nizioł-Łukaszewska Magdalena Wójciak Ireneusz Sowa |
| author_sort | Martyna Zagórska-Dziok |
| collection | DOAJ |
| description | Due to the growing popularity of herbal extract-loaded hydrogels, this study assessed the biological activity of extracts and hydrogels containing three types (water (WE), water–ethanol (EE) and water–glycerin (GE)) of <i>Cornus mas</i> L. (dogwood) extracts. The content of biologically active compounds in the extracts was assessed using the UPLC-DAD-MS technique. Antioxidant properties were assessed by using DPPH and ABTS radicals and measuring the intracellular level of reactive oxygen species. Alamar Blue and Neutral Red tests were used to measure the cytotoxicity of the tested samples on skin cells—fibroblasts and keratinocytes. Cell migration and the anti-aging activity of the tested extracts and hydrogels were assessed. Transepidermal water loss and skin hydration after applying the hydrogels to the skin were also determined. A chromatographic analysis revealed that the extracts contained polyphenols, including gallic, caftaric, protocatechuic, chlorogenic, ellagic and p-coumaroylquinic acids, as well as iridoids, with loganic acid as the predominant component. Additionally, they contained cyanidin 3-<i>O</i>-galactoside, pelargonidin 3-<i>O</i>-glucoside and quinic acid. The obtained results show that the tested extracts and hydrogels had strong antioxidant properties and had a positive effect on the viability of skin cells in vitro. Additionally, it was shown that they stimulated the migration of these cells and had the ability to inhibit the activity of collagenase and elastase. Moreover, the tested hydrogels increased skin hydration and prevented transepidermal water loss. The obtained results indicate that the developed hydrogels may be effective delivery systems for phytochemicals contained in dogwood extracts. |
| first_indexed | 2024-03-11T11:24:05Z |
| format | Article |
| id | doaj.art-2e4343cae3e6401693d593f91bb20ecc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T11:24:05Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2e4343cae3e6401693d593f91bb20ecc2023-11-10T15:08:49ZengMDPI AGMolecules1420-30492023-11-012821738410.3390/molecules28217384Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and CosmetologyMartyna Zagórska-Dziok0Aleksandra Ziemlewska1Agnieszka Mokrzyńska2Zofia Nizioł-Łukaszewska3Magdalena Wójciak4Ireneusz Sowa5Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, PolandDepartment of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, PolandDepartment of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, PolandDepartment of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Aleje Raclawickie 1, 20-059 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Aleje Raclawickie 1, 20-059 Lublin, PolandDue to the growing popularity of herbal extract-loaded hydrogels, this study assessed the biological activity of extracts and hydrogels containing three types (water (WE), water–ethanol (EE) and water–glycerin (GE)) of <i>Cornus mas</i> L. (dogwood) extracts. The content of biologically active compounds in the extracts was assessed using the UPLC-DAD-MS technique. Antioxidant properties were assessed by using DPPH and ABTS radicals and measuring the intracellular level of reactive oxygen species. Alamar Blue and Neutral Red tests were used to measure the cytotoxicity of the tested samples on skin cells—fibroblasts and keratinocytes. Cell migration and the anti-aging activity of the tested extracts and hydrogels were assessed. Transepidermal water loss and skin hydration after applying the hydrogels to the skin were also determined. A chromatographic analysis revealed that the extracts contained polyphenols, including gallic, caftaric, protocatechuic, chlorogenic, ellagic and p-coumaroylquinic acids, as well as iridoids, with loganic acid as the predominant component. Additionally, they contained cyanidin 3-<i>O</i>-galactoside, pelargonidin 3-<i>O</i>-glucoside and quinic acid. The obtained results show that the tested extracts and hydrogels had strong antioxidant properties and had a positive effect on the viability of skin cells in vitro. Additionally, it was shown that they stimulated the migration of these cells and had the ability to inhibit the activity of collagenase and elastase. Moreover, the tested hydrogels increased skin hydration and prevented transepidermal water loss. The obtained results indicate that the developed hydrogels may be effective delivery systems for phytochemicals contained in dogwood extracts.https://www.mdpi.com/1420-3049/28/21/7384<i>Cornus mas</i> L.skin cellshydrogelsantioxidantsskin agingcollagenase |
| spellingShingle | Martyna Zagórska-Dziok Aleksandra Ziemlewska Agnieszka Mokrzyńska Zofia Nizioł-Łukaszewska Magdalena Wójciak Ireneusz Sowa Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and Cosmetology Molecules <i>Cornus mas</i> L. skin cells hydrogels antioxidants skin aging collagenase |
| title | Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and Cosmetology |
| title_full | Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and Cosmetology |
| title_fullStr | Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and Cosmetology |
| title_full_unstemmed | Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and Cosmetology |
| title_short | Evaluation of the Biological Activity of Hydrogel with <i>Cornus mas</i> L. Extract and Its Potential Use in Dermatology and Cosmetology |
| title_sort | evaluation of the biological activity of hydrogel with i cornus mas i l extract and its potential use in dermatology and cosmetology |
| topic | <i>Cornus mas</i> L. skin cells hydrogels antioxidants skin aging collagenase |
| url | https://www.mdpi.com/1420-3049/28/21/7384 |
| work_keys_str_mv | AT martynazagorskadziok evaluationofthebiologicalactivityofhydrogelwithicornusmasilextractanditspotentialuseindermatologyandcosmetology AT aleksandraziemlewska evaluationofthebiologicalactivityofhydrogelwithicornusmasilextractanditspotentialuseindermatologyandcosmetology AT agnieszkamokrzynska evaluationofthebiologicalactivityofhydrogelwithicornusmasilextractanditspotentialuseindermatologyandcosmetology AT zofianiziołłukaszewska evaluationofthebiologicalactivityofhydrogelwithicornusmasilextractanditspotentialuseindermatologyandcosmetology AT magdalenawojciak evaluationofthebiologicalactivityofhydrogelwithicornusmasilextractanditspotentialuseindermatologyandcosmetology AT ireneuszsowa evaluationofthebiologicalactivityofhydrogelwithicornusmasilextractanditspotentialuseindermatologyandcosmetology |