A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method
Glucosinolates (GLSs) are a well-studied sulfur-containing compound found in <i>Brassicaceae</i> plants that play critical roles in plant resistance and human health. Correctly identifying and reliably quantifying the total and individual GLS content is of great importance. An improved m...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/5/650 |
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| author | Yanfang Li Mengliang Zhang Pamela Pehrsson James M. Harnly Pei Chen Jianghao Sun |
| author_facet | Yanfang Li Mengliang Zhang Pamela Pehrsson James M. Harnly Pei Chen Jianghao Sun |
| author_sort | Yanfang Li |
| collection | DOAJ |
| description | Glucosinolates (GLSs) are a well-studied sulfur-containing compound found in <i>Brassicaceae</i> plants that play critical roles in plant resistance and human health. Correctly identifying and reliably quantifying the total and individual GLS content is of great importance. An improved method as an alternative to the ISO 9167-1 (ISO) method is developed in the present study. An efficient extraction and purification procedure is proposed with a commercially available dimethylaminopropyl (DEA)-based weak anion exchange solid-phase extraction (SPE) cartridge instead of using the self-prepared ion-exchange columns in the ISO method. The GLSs are identified and quantified by ultra high-performance liquid chromatography (UHPLC) high-resolution mass spectrometry (HRMS). The method demonstrates a comparable quantification of total and individual GLSs on certified rapeseeds and other <i>Brassicaceae</i> vegetables when compared to the ISO method. The developed SPE method is simpler and more efficient, thus allowing for applications to a large sample size with reduced analysis time, improved repeatability and accuracy, and possible automation. |
| first_indexed | 2024-04-25T00:30:54Z |
| format | Article |
| id | doaj.art-2e465c8a23e843fb81b25cb620e6e673 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-25T00:30:54Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2e465c8a23e843fb81b25cb620e6e6732024-03-12T16:44:00ZengMDPI AGFoods2304-81582024-02-0113565010.3390/foods13050650A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 MethodYanfang Li0Mengliang Zhang1Pamela Pehrsson2James M. Harnly3Pei Chen4Jianghao Sun5Methods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USADepartment of Chemistry, Middle Tennessee State University, Murfreesboro, TN 37132, USAMethods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAMethods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAMethods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAMethods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAGlucosinolates (GLSs) are a well-studied sulfur-containing compound found in <i>Brassicaceae</i> plants that play critical roles in plant resistance and human health. Correctly identifying and reliably quantifying the total and individual GLS content is of great importance. An improved method as an alternative to the ISO 9167-1 (ISO) method is developed in the present study. An efficient extraction and purification procedure is proposed with a commercially available dimethylaminopropyl (DEA)-based weak anion exchange solid-phase extraction (SPE) cartridge instead of using the self-prepared ion-exchange columns in the ISO method. The GLSs are identified and quantified by ultra high-performance liquid chromatography (UHPLC) high-resolution mass spectrometry (HRMS). The method demonstrates a comparable quantification of total and individual GLSs on certified rapeseeds and other <i>Brassicaceae</i> vegetables when compared to the ISO method. The developed SPE method is simpler and more efficient, thus allowing for applications to a large sample size with reduced analysis time, improved repeatability and accuracy, and possible automation.https://www.mdpi.com/2304-8158/13/5/650glucosinolatesquantificationISO 9167 method<i>Brassicaceae</i> vegetablesdimethylaminopropyl (DEA)weak anion exchange |
| spellingShingle | Yanfang Li Mengliang Zhang Pamela Pehrsson James M. Harnly Pei Chen Jianghao Sun A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method Foods glucosinolates quantification ISO 9167 method <i>Brassicaceae</i> vegetables dimethylaminopropyl (DEA) weak anion exchange |
| title | A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method |
| title_full | A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method |
| title_fullStr | A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method |
| title_full_unstemmed | A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method |
| title_short | A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method |
| title_sort | fast and simple solid phase extraction based method for glucosinolate determination an alternative to the iso 9167 method |
| topic | glucosinolates quantification ISO 9167 method <i>Brassicaceae</i> vegetables dimethylaminopropyl (DEA) weak anion exchange |
| url | https://www.mdpi.com/2304-8158/13/5/650 |
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