Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape)
γ-Aminobutyric acid (GABA) is a very important metabolite which plays a crucial role in signal transmission, stress metabolism and some other activities are also reported. Although the actual role of GABA shunt is not clearly understood. Three key enzymes, gamma aminobutaric acid transam...
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2019-04-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=12269 |
| _version_ | 1818436753604739072 |
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| author | Md. Shahjahan Shahidur Rahman |
| author_facet | Md. Shahjahan Shahidur Rahman |
| author_sort | Md. Shahjahan |
| collection | DOAJ |
| description | γ-Aminobutyric acid (GABA) is a very important metabolite which plays a crucial role in signal transmission, stress metabolism and some other activities are also reported. Although the actual role of GABA shunt is not clearly understood. Three key enzymes, gamma aminobutaric acid transaminase (GABA-T), succinic semialdehyde dehydrogenase and succinic semialdehyde reductase are involved in GABA shunt mechanism en route from glutamate to the tricaboxylic acid cycle (TCA) which can pave the way of GABA shunt action. The enzyme gamma aminobutaric acid transaminase (GABA-T) plays a key role in GABA shunt action by converting of GABA to succinic semialdehyde (SSA). In this study the protein sequence of γ-Aminobutyric acid transaminase of Brassica napus (Rape) is retrieved from UniProt protein database and analyzed GABA-T enzyme using different bioinformatics tools and servers to analyze the physiochemical properties, amino acid composition, conformational states and 3D structure. We found that our experimental protein sequence is very unstable and the graph of Local Quality Estimate shows that the sequence is porn to mutation and value of Z score is above two in comparison with non-redundant set of PDB structure. In addition, the phylogenetic tree reveals that GABA-T of Malus domestica Solanum lycopersicum , Oryza sativa , Arabidopsis thaliana and Brassica napus were evolved from a common ancestor gene. [ J Adv Biotechnol Exp Ther 2019; 2(1.000): 04-09] |
| first_indexed | 2024-12-14T17:13:47Z |
| format | Article |
| id | doaj.art-2e48e1daff9247f5a17947ff384b01ef |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-12-14T17:13:47Z |
| publishDate | 2019-04-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-2e48e1daff9247f5a17947ff384b01ef2022-12-21T22:53:28ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602019-04-0121040910.5455/jabet.2018.d1812269Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape)Md. Shahjahan0Shahidur Rahman1Bangladesh Livestock Research Institute, Regional Station, Baghabari, Shahjadpur, Sirajganj-6770, Bangladesh Bangladesh Livestock Research Institute, Regional Station, Baghabari, Shahjadpur, Sirajganj-6770, Bangladeshγ-Aminobutyric acid (GABA) is a very important metabolite which plays a crucial role in signal transmission, stress metabolism and some other activities are also reported. Although the actual role of GABA shunt is not clearly understood. Three key enzymes, gamma aminobutaric acid transaminase (GABA-T), succinic semialdehyde dehydrogenase and succinic semialdehyde reductase are involved in GABA shunt mechanism en route from glutamate to the tricaboxylic acid cycle (TCA) which can pave the way of GABA shunt action. The enzyme gamma aminobutaric acid transaminase (GABA-T) plays a key role in GABA shunt action by converting of GABA to succinic semialdehyde (SSA). In this study the protein sequence of γ-Aminobutyric acid transaminase of Brassica napus (Rape) is retrieved from UniProt protein database and analyzed GABA-T enzyme using different bioinformatics tools and servers to analyze the physiochemical properties, amino acid composition, conformational states and 3D structure. We found that our experimental protein sequence is very unstable and the graph of Local Quality Estimate shows that the sequence is porn to mutation and value of Z score is above two in comparison with non-redundant set of PDB structure. In addition, the phylogenetic tree reveals that GABA-T of Malus domestica Solanum lycopersicum , Oryza sativa , Arabidopsis thaliana and Brassica napus were evolved from a common ancestor gene. [ J Adv Biotechnol Exp Ther 2019; 2(1.000): 04-09]http://www.ejmanager.com/fulltextpdf.php?mno=12269gaba-ttca cycleuniportbioinformaticsmetabolismtransmissionstress |
| spellingShingle | Md. Shahjahan Shahidur Rahman Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape) Journal of Advanced Biotechnology and Experimental Therapeutics gaba-t tca cycle uniport bioinformatics metabolism transmission stress |
| title | Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape) |
| title_full | Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape) |
| title_fullStr | Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape) |
| title_full_unstemmed | Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape) |
| title_short | Insilico Analysis of γ- Aminobutaric Acid Transaminase (GABA-T) of Brassica napus (Rape) |
| title_sort | insilico analysis of 947 aminobutaric acid transaminase gaba t of brassica napus rape |
| topic | gaba-t tca cycle uniport bioinformatics metabolism transmission stress |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=12269 |
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