Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South Lampung
Thermophilic bacteria are microorganisms with the capability to produce a specific enzyme known as amylase, which exhibits resistance to heat and is referred to as thermostable. This study aims to isolate, characterize, and assess the enzyme-producing activity of bacteria from the hot springs of Way...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/12/e3sconf_ysssee2024_03007.pdf |
| _version_ | 1797334036073414656 |
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| author | Sugiharta Iip Satiyarti Rina Budi Safitri Lina Rinaldi Achi Suri Fraulein Intan |
| author_facet | Sugiharta Iip Satiyarti Rina Budi Safitri Lina Rinaldi Achi Suri Fraulein Intan |
| author_sort | Sugiharta Iip |
| collection | DOAJ |
| description | Thermophilic bacteria are microorganisms with the capability to produce a specific enzyme known as amylase, which exhibits resistance to heat and is referred to as thermostable. This study aims to isolate, characterize, and assess the enzyme-producing activity of bacteria from the hot springs of Way Belerang, Lampung, specifically amylase. The research procedures encompassed sample collection, isolation of thermophilic bacteria incubated in NA media for 48 hours, amylum testing with iodine after a 24-hour incubation in NA media containing starch/amylose, biochemical tests including TSIA, SIM, SC, urea test, sugar fermentation test, and catalase test. The results of the study yielded two isolates successfully isolated from the hot spring source, and Gram staining indicated that both isolates were Gram-negative bacteria. The assessment of bacterial activity in producing amylase was demonstrated by the presence of clear zones, with the largest bacterial diameter measuring 1.04 mm as measured using a vernier caliper. Characterization of the bacteria identified them as belonging to the Pseudomonas genus for isolates AB2 and AB3. Thus, the bacteria obtained in this study produce amylase enzymes that can be utilized in various industrial applications, including the food, beverage, textile, detergent, paper, pharmaceutical, and other sectors. |
| first_indexed | 2024-03-08T08:14:14Z |
| format | Article |
| id | doaj.art-2e4b13fa9bbe4512b93607f21b7d84ca |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-08T08:14:14Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-2e4b13fa9bbe4512b93607f21b7d84ca2024-02-02T07:56:55ZengEDP SciencesE3S Web of Conferences2267-12422024-01-014820300710.1051/e3sconf/202448203007e3sconf_ysssee2024_03007Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South LampungSugiharta Iip0Satiyarti Rina Budi1Safitri Lina2Rinaldi Achi3Suri Fraulein Intan4Universitas Islam Negeri Raden Intan LampungUniversitas Islam Negeri Sunan Gunung Djati BandungUniversitas Islam Negeri Raden Intan LampungUniversitas Islam Negeri Raden Intan LampungUniversitas Islam Negeri Raden Intan LampungThermophilic bacteria are microorganisms with the capability to produce a specific enzyme known as amylase, which exhibits resistance to heat and is referred to as thermostable. This study aims to isolate, characterize, and assess the enzyme-producing activity of bacteria from the hot springs of Way Belerang, Lampung, specifically amylase. The research procedures encompassed sample collection, isolation of thermophilic bacteria incubated in NA media for 48 hours, amylum testing with iodine after a 24-hour incubation in NA media containing starch/amylose, biochemical tests including TSIA, SIM, SC, urea test, sugar fermentation test, and catalase test. The results of the study yielded two isolates successfully isolated from the hot spring source, and Gram staining indicated that both isolates were Gram-negative bacteria. The assessment of bacterial activity in producing amylase was demonstrated by the presence of clear zones, with the largest bacterial diameter measuring 1.04 mm as measured using a vernier caliper. Characterization of the bacteria identified them as belonging to the Pseudomonas genus for isolates AB2 and AB3. Thus, the bacteria obtained in this study produce amylase enzymes that can be utilized in various industrial applications, including the food, beverage, textile, detergent, paper, pharmaceutical, and other sectors.https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/12/e3sconf_ysssee2024_03007.pdf |
| spellingShingle | Sugiharta Iip Satiyarti Rina Budi Safitri Lina Rinaldi Achi Suri Fraulein Intan Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South Lampung E3S Web of Conferences |
| title | Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South Lampung |
| title_full | Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South Lampung |
| title_fullStr | Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South Lampung |
| title_full_unstemmed | Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South Lampung |
| title_short | Identification and Study of Thermophilic Bacteria Found in the Sulfur-Infused Hot Springs of South Lampung |
| title_sort | identification and study of thermophilic bacteria found in the sulfur infused hot springs of south lampung |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/12/e3sconf_ysssee2024_03007.pdf |
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