Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering
Tooth loss has been found to adversely affect not just masticatory and speech functions, but also psychological health and quality of life. Currently, teeth replacement options include dentures, bridges, and implants. However, these artificial replacement options remain inferior to biological replac...
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MDPI AG
2021-08-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/7/3/123 |
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| author | Sol Park Naomi W. Y. Huang Cheryl X. Y. Wong Jing Pan Lamyaa Albakr Jing Gu Lifeng Kang |
| author_facet | Sol Park Naomi W. Y. Huang Cheryl X. Y. Wong Jing Pan Lamyaa Albakr Jing Gu Lifeng Kang |
| author_sort | Sol Park |
| collection | DOAJ |
| description | Tooth loss has been found to adversely affect not just masticatory and speech functions, but also psychological health and quality of life. Currently, teeth replacement options include dentures, bridges, and implants. However, these artificial replacement options remain inferior to biological replacements due to their reduced efficiency, the need for replacements, and the risk of immunological rejection. To this end, there has been a heightened interest in the bioengineering of teeth in recent years. While there have been reports of successfully regenerated teeth, controlling the size and shape of bioengineered teeth remains a challenge. In this study, methacrylated hyaluronic acid (MeHA) was synthesized and microstructured in a hydrogel microwell array using soft lithography. The resulting MeHA hydrogel microwell scaffold resembles the shape of a naturally developing human tooth germ. To facilitate the epithelial–mesenchymal interactions, human adult low calcium high temperature (HaCaT) cells were seeded on the surface of the hydrogels and dental pulp stem cells (DPSCs) were encapsulated inside the hydrogels. It was found that hydrogel scaffolds were able to preserve the viability of both types of cells and they appeared to favor signaling between epithelial and mesenchymal cells, which is necessary in the promotion of cell proliferation. As such, the hydrogel scaffolds offer a promising system for the bioengineering of human tooth germs in vitro. |
| first_indexed | 2024-03-10T07:38:27Z |
| format | Article |
| id | doaj.art-2e51a287aff74ba98fbd75384121315b |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T07:38:27Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-2e51a287aff74ba98fbd75384121315b2023-11-22T13:11:58ZengMDPI AGGels2310-28612021-08-017312310.3390/gels7030123Microstructured Hyaluronic Acid Hydrogel for Tooth Germ BioengineeringSol Park0Naomi W. Y. Huang1Cheryl X. Y. Wong2Jing Pan3Lamyaa Albakr4Jing Gu5Lifeng Kang6School of Pharmacy, Faculty of Medicine and Health, University of Sydney, Pharmacy and Bank Building A15, Science Road, Sydney, NSW 2006, AustraliaDepartment of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, SingaporeDepartment of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, SingaporeSkinetrate Pte. Ltd., 79 Ayer Rajah Crescent, Singapore 139955, SingaporeDepartment of Pharmaceutics, King Saud University, Riyadh 11454, Saudi ArabiaDepartment of Dentistry, The Sixth Medical Centre of PLA General Hospital, 6 Fucheng Road, Haidian District, Beijing 100048, ChinaSchool of Pharmacy, Faculty of Medicine and Health, University of Sydney, Pharmacy and Bank Building A15, Science Road, Sydney, NSW 2006, AustraliaTooth loss has been found to adversely affect not just masticatory and speech functions, but also psychological health and quality of life. Currently, teeth replacement options include dentures, bridges, and implants. However, these artificial replacement options remain inferior to biological replacements due to their reduced efficiency, the need for replacements, and the risk of immunological rejection. To this end, there has been a heightened interest in the bioengineering of teeth in recent years. While there have been reports of successfully regenerated teeth, controlling the size and shape of bioengineered teeth remains a challenge. In this study, methacrylated hyaluronic acid (MeHA) was synthesized and microstructured in a hydrogel microwell array using soft lithography. The resulting MeHA hydrogel microwell scaffold resembles the shape of a naturally developing human tooth germ. To facilitate the epithelial–mesenchymal interactions, human adult low calcium high temperature (HaCaT) cells were seeded on the surface of the hydrogels and dental pulp stem cells (DPSCs) were encapsulated inside the hydrogels. It was found that hydrogel scaffolds were able to preserve the viability of both types of cells and they appeared to favor signaling between epithelial and mesenchymal cells, which is necessary in the promotion of cell proliferation. As such, the hydrogel scaffolds offer a promising system for the bioengineering of human tooth germs in vitro.https://www.mdpi.com/2310-2861/7/3/123hyaluronic acidhydrogeltooth developmentepithelial–mesenchymal interactionhuman dental pulp stem cellssoft lithography |
| spellingShingle | Sol Park Naomi W. Y. Huang Cheryl X. Y. Wong Jing Pan Lamyaa Albakr Jing Gu Lifeng Kang Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering Gels hyaluronic acid hydrogel tooth development epithelial–mesenchymal interaction human dental pulp stem cells soft lithography |
| title | Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering |
| title_full | Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering |
| title_fullStr | Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering |
| title_full_unstemmed | Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering |
| title_short | Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering |
| title_sort | microstructured hyaluronic acid hydrogel for tooth germ bioengineering |
| topic | hyaluronic acid hydrogel tooth development epithelial–mesenchymal interaction human dental pulp stem cells soft lithography |
| url | https://www.mdpi.com/2310-2861/7/3/123 |
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