Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6
Induction of metallothionein synthesis in the presence or absence of Interleukin –6 (IL-6) was one of the responses examined to identify the mechanism by which IL-6 (at high concentration) was able to reverse cadmium inhibition of B9 cell line proliferation. Cellular metallothionein was assayed by C...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2005-09-01
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| Series: | Journal of Applied Sciences and Environmental Management |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/17229 |
| _version_ | 1797227817873702912 |
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| author | I Orupabo |
| author_facet | I Orupabo |
| author_sort | I Orupabo |
| collection | DOAJ |
| description | Induction of metallothionein synthesis in the presence or absence of Interleukin –6 (IL-6) was one of the responses examined to identify the mechanism by which IL-6 (at high concentration) was able to reverse cadmium inhibition of B9 cell line proliferation. Cellular metallothionein was assayed by Cd-haem method. The results revealed a Cd-induced increase in cellular metallothionein synthesis. IL-6 alone was not sufficient to induce expression of metallothionein in this cell line. The Kinetics of B9 cells handling of cadmium (using radio active 109Cd as tracer) was another response examined. Cells were pre-loaded with 109Cd then transferred to 109Cd-free medium containing IL-6. The results show the efflux of Cd from IL-6 stimulated B9 cells, which suggest that there is no functional linkage between IL-6 stimulation and cellular handling of Cd. The efflux of cadmium out of the cells is however an important observation in that many function assays used in immunotoxicology are carried out in-vitro after cellular exposure to the chemical in-vivo. In the interpretation of such data, the ability of the cells to recover as a result of cellular efflux of the chemical should always be considered.
Journal of Applied Sciences and Environmental Management Vol. 8 (1) 2004:63 - 65 |
| first_indexed | 2024-04-24T14:46:50Z |
| format | Article |
| id | doaj.art-2e53c68527194d17abcfe645d007ac7b |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:46:50Z |
| publishDate | 2005-09-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-2e53c68527194d17abcfe645d007ac7b2024-04-02T19:54:58ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992005-09-018110.4314/jasem.v8i1.17229Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6I Orupabo0Department. Of Chemical Pathology, University of Port Harcourt, Uniport P. O. Box 449, Choba, Port Harcourt, NigeriaInduction of metallothionein synthesis in the presence or absence of Interleukin –6 (IL-6) was one of the responses examined to identify the mechanism by which IL-6 (at high concentration) was able to reverse cadmium inhibition of B9 cell line proliferation. Cellular metallothionein was assayed by Cd-haem method. The results revealed a Cd-induced increase in cellular metallothionein synthesis. IL-6 alone was not sufficient to induce expression of metallothionein in this cell line. The Kinetics of B9 cells handling of cadmium (using radio active 109Cd as tracer) was another response examined. Cells were pre-loaded with 109Cd then transferred to 109Cd-free medium containing IL-6. The results show the efflux of Cd from IL-6 stimulated B9 cells, which suggest that there is no functional linkage between IL-6 stimulation and cellular handling of Cd. The efflux of cadmium out of the cells is however an important observation in that many function assays used in immunotoxicology are carried out in-vitro after cellular exposure to the chemical in-vivo. In the interpretation of such data, the ability of the cells to recover as a result of cellular efflux of the chemical should always be considered. Journal of Applied Sciences and Environmental Management Vol. 8 (1) 2004:63 - 65https://www.ajol.info/index.php/jasem/article/view/17229 |
| spellingShingle | I Orupabo Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6 Journal of Applied Sciences and Environmental Management |
| title | Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6 |
| title_full | Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6 |
| title_fullStr | Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6 |
| title_full_unstemmed | Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6 |
| title_short | Evaluation of Hybridoma B9 cell line handling of Cadmium in the presence of Interleukin-6 |
| title_sort | evaluation of hybridoma b9 cell line handling of cadmium in the presence of interleukin 6 |
| url | https://www.ajol.info/index.php/jasem/article/view/17229 |
| work_keys_str_mv | AT iorupabo evaluationofhybridomab9celllinehandlingofcadmiuminthepresenceofinterleukin6 |