CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis
ABSTRACTRapid and simple-to-use diagnostic methods for tuberculosis are urgently needed. Recent development has unveiled the diagnostic power of the CRISPR system in the detection of viral infections. However, its potential use in detecting the Mycobacterium tuberculosis complex (MTB) remained unexp...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1664939 |
| _version_ | 1797385751049011200 |
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| author | Jing-Wen Ai Xian Zhou Teng Xu Minling Yang Yuanyuan Chen Gui-Qing He Ningp Pan Yuwei Cai Yongjun Li Xiaorui Wang Hang Su Ting Wang Weiqi Zeng Wen-Hong Zhang |
| author_facet | Jing-Wen Ai Xian Zhou Teng Xu Minling Yang Yuanyuan Chen Gui-Qing He Ningp Pan Yuwei Cai Yongjun Li Xiaorui Wang Hang Su Ting Wang Weiqi Zeng Wen-Hong Zhang |
| author_sort | Jing-Wen Ai |
| collection | DOAJ |
| description | ABSTRACTRapid and simple-to-use diagnostic methods for tuberculosis are urgently needed. Recent development has unveiled the diagnostic power of the CRISPR system in the detection of viral infections. However, its potential use in detecting the Mycobacterium tuberculosis complex (MTB) remained unexplored. We developed a rapid CRISPR-based assay for TB detection and conducted a retrospective cohort study of 179 patients to evaluate the CRISPR-MTB test for identifying MTB in various forms of direct clinical samples. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the clinical cohort of both pulmonary and extra-pulmonary tuberculosis, the CRISPR-MTB test exhibited an overall improved sensitivity over both culture (79% vs 33%) and Xpert (79% vs 66%), without comprise in specificity (62/63, 98%). The CRISPR-MTB test exhibits an improved overall diagnostic performance over culture and Xpert across a variety of sample types, and offers great potential as a new diagnostic technique for both pulmonary and extra-pulmonary tuberculosis. |
| first_indexed | 2024-03-08T21:58:47Z |
| format | Article |
| id | doaj.art-2e53cf13994e414cb65d8939cefd8441 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:58:47Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-2e53cf13994e414cb65d8939cefd84412023-12-19T16:09:58ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01811361136910.1080/22221751.2019.1664939CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosisJing-Wen Ai0Xian Zhou1Teng Xu2Minling Yang3Yuanyuan Chen4Gui-Qing He5Ningp Pan6Yuwei Cai7Yongjun Li8Xiaorui Wang9Hang Su10Ting Wang11Weiqi Zeng12Wen-Hong Zhang13Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaVision Medicals Co., Ltd, Guangzhou, People’s Republic of ChinaVision Medicals Co., Ltd, Guangzhou, People’s Republic of ChinaTuberculosis Diagnosis and Treatment Center, Hangzhou Red Cross Hospital, Hangzhou, People’s Republic of ChinaDepartment of Infectious Diseases, Wenzhou Central Hospital, Wenzhou, People’s Republic of ChinaDepartment of Infectious Diseases, Wenzhou Central Hospital, Wenzhou, People’s Republic of ChinaDepartment of Infectious Diseases, Wenzhou Central Hospital, Wenzhou, People’s Republic of ChinaVision Medicals Co., Ltd, Guangzhou, People’s Republic of ChinaVision Medicals Co., Ltd, Guangzhou, People’s Republic of ChinaVision Medicals Co., Ltd, Guangzhou, People’s Republic of ChinaDepartment of Infectious Diseases, Jing’an District Central Hospital of Shanghai, Shanghai, People’s Republic of ChinaVision Medicals Co., Ltd, Guangzhou, People’s Republic of ChinaDepartment of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaABSTRACTRapid and simple-to-use diagnostic methods for tuberculosis are urgently needed. Recent development has unveiled the diagnostic power of the CRISPR system in the detection of viral infections. However, its potential use in detecting the Mycobacterium tuberculosis complex (MTB) remained unexplored. We developed a rapid CRISPR-based assay for TB detection and conducted a retrospective cohort study of 179 patients to evaluate the CRISPR-MTB test for identifying MTB in various forms of direct clinical samples. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the clinical cohort of both pulmonary and extra-pulmonary tuberculosis, the CRISPR-MTB test exhibited an overall improved sensitivity over both culture (79% vs 33%) and Xpert (79% vs 66%), without comprise in specificity (62/63, 98%). The CRISPR-MTB test exhibits an improved overall diagnostic performance over culture and Xpert across a variety of sample types, and offers great potential as a new diagnostic technique for both pulmonary and extra-pulmonary tuberculosis.https://www.tandfonline.com/doi/10.1080/22221751.2019.1664939CRISPR-MTBtuberculosisMycobacterium tuberculosis complex (MTB)GeneXpert MTB/RIFdiagnosis |
| spellingShingle | Jing-Wen Ai Xian Zhou Teng Xu Minling Yang Yuanyuan Chen Gui-Qing He Ningp Pan Yuwei Cai Yongjun Li Xiaorui Wang Hang Su Ting Wang Weiqi Zeng Wen-Hong Zhang CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis Emerging Microbes and Infections CRISPR-MTB tuberculosis Mycobacterium tuberculosis complex (MTB) GeneXpert MTB/RIF diagnosis |
| title | CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis |
| title_full | CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis |
| title_fullStr | CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis |
| title_full_unstemmed | CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis |
| title_short | CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis |
| title_sort | crispr based rapid and ultra sensitive diagnostic test for mycobacterium tuberculosis |
| topic | CRISPR-MTB tuberculosis Mycobacterium tuberculosis complex (MTB) GeneXpert MTB/RIF diagnosis |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1664939 |
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