Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli
Crop production often faces challenges from plant diseases, and biological control emerges as an effective, environmentally friendly, cost-effective, and sustainable alternative to chemical control. Wheat blast disease caused by fungal pathogen Magnaporthe oryzae Triticum (MoT), is a potential catas...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1336515/full |
| _version_ | 1797266411038441472 |
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| author | Musrat Zahan Surovy Sudipta Dutta Nur Uddin Mahmud Dipali Rani Gupta Tarin Farhana Sanjay Kumar Paul Joe Win Christopher Dunlap Ricardo Oliva Mahfuzur Rahman Andrew G. Sharpe Tofazzal Islam |
| author_facet | Musrat Zahan Surovy Sudipta Dutta Nur Uddin Mahmud Dipali Rani Gupta Tarin Farhana Sanjay Kumar Paul Joe Win Christopher Dunlap Ricardo Oliva Mahfuzur Rahman Andrew G. Sharpe Tofazzal Islam |
| author_sort | Musrat Zahan Surovy |
| collection | DOAJ |
| description | Crop production often faces challenges from plant diseases, and biological control emerges as an effective, environmentally friendly, cost-effective, and sustainable alternative to chemical control. Wheat blast disease caused by fungal pathogen Magnaporthe oryzae Triticum (MoT), is a potential catastrophic threat to global food security. This study aimed to identify potential bacterial isolates from rice and wheat seeds with inhibitory effects against MoT. In dual culture and seedling assays, three bacterial isolates (BTS-3, BTS-4, and BTLK6A) demonstrated effective suppression of MoT growth and reduced wheat blast severity when artificially inoculated at the seedling stage. Genome phylogeny identified these isolates as Bacillus subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A). Whole-genome analysis revealed the presence of genes responsible for controlling MoT through antimicrobial defense, antioxidant defense, cell wall degradation, and induced systemic resistance (ISR). Taken together, our results suggest that the suppression of wheat blast disease by seed endophytic B. subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A) is liked with antibiosis and induced systemic resistance to wheat plants. A further field validation is needed before recommending these endophytic bacteria for biological control of wheat blast. |
| first_indexed | 2024-04-25T01:00:15Z |
| format | Article |
| id | doaj.art-2e584e311ccb48268d5eee227a07714a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-25T01:00:15Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2e584e311ccb48268d5eee227a07714a2024-03-11T04:42:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13365151336515Biological control potential of worrisome wheat blast disease by the seed endophytic bacilliMusrat Zahan Surovy0Sudipta Dutta1Nur Uddin Mahmud2Dipali Rani Gupta3Tarin Farhana4Sanjay Kumar Paul5Joe Win6Christopher Dunlap7Ricardo Oliva8Mahfuzur Rahman9Andrew G. Sharpe10Tofazzal Islam11Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshThe Sainsbury Laboratory, Norwich Research Park, Norwich, United KingdomCrop Bioprotection Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture (USDA), Peoria, IL, United StatesWorld Vegetable Center, Shanhua, TaiwanW.V.U. Extension Service, West Virginia University, Morgantown, WV, United StatesGlobal Institute for Food Security, Saskatoon, SK, CanadaInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshCrop production often faces challenges from plant diseases, and biological control emerges as an effective, environmentally friendly, cost-effective, and sustainable alternative to chemical control. Wheat blast disease caused by fungal pathogen Magnaporthe oryzae Triticum (MoT), is a potential catastrophic threat to global food security. This study aimed to identify potential bacterial isolates from rice and wheat seeds with inhibitory effects against MoT. In dual culture and seedling assays, three bacterial isolates (BTS-3, BTS-4, and BTLK6A) demonstrated effective suppression of MoT growth and reduced wheat blast severity when artificially inoculated at the seedling stage. Genome phylogeny identified these isolates as Bacillus subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A). Whole-genome analysis revealed the presence of genes responsible for controlling MoT through antimicrobial defense, antioxidant defense, cell wall degradation, and induced systemic resistance (ISR). Taken together, our results suggest that the suppression of wheat blast disease by seed endophytic B. subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A) is liked with antibiosis and induced systemic resistance to wheat plants. A further field validation is needed before recommending these endophytic bacteria for biological control of wheat blast.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1336515/fullbacillusantagonistblast severitygenomeantimicrobial defenseantibiosis |
| spellingShingle | Musrat Zahan Surovy Sudipta Dutta Nur Uddin Mahmud Dipali Rani Gupta Tarin Farhana Sanjay Kumar Paul Joe Win Christopher Dunlap Ricardo Oliva Mahfuzur Rahman Andrew G. Sharpe Tofazzal Islam Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli Frontiers in Microbiology bacillus antagonist blast severity genome antimicrobial defense antibiosis |
| title | Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli |
| title_full | Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli |
| title_fullStr | Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli |
| title_full_unstemmed | Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli |
| title_short | Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli |
| title_sort | biological control potential of worrisome wheat blast disease by the seed endophytic bacilli |
| topic | bacillus antagonist blast severity genome antimicrobial defense antibiosis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1336515/full |
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