First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey
The Pacific oyster (Crassostrea gigas) has a very important economic potential for aquaculture, but on the other hand, is among the highly invasive species in the world and within the Mediterranean ecosystem. In the 1960s, C. gigas was brought to Europe for aquaculture in the Mediterranean and Black...
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| Format: | Article |
| Language: | English |
| Published: |
Adem Yavuz Sönmez
2020-06-01
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| Series: | Marine Science and Technology Bulletin |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/pub/masteb/issue/50769/668529?publisher=ademyavuzsonmez |
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| author | Emel ÖZCAN GÖKÇEK Sefa ACARLI Bilge KARAHAN Pervin VURAL Evren KOBAN BAŞTANLAR |
| author_facet | Emel ÖZCAN GÖKÇEK Sefa ACARLI Bilge KARAHAN Pervin VURAL Evren KOBAN BAŞTANLAR |
| author_sort | Emel ÖZCAN GÖKÇEK |
| collection | DOAJ |
| description | The Pacific oyster (Crassostrea
gigas) has a very important economic potential for aquaculture, but on the
other hand, is among the highly invasive species in the world and within the
Mediterranean ecosystem. In the 1960s, C. gigas was brought to Europe
for aquaculture in the Mediterranean and Black Sea regions from Japan and
Canada. The Turkish waters are the part of the Mediterranean Sea, which is the
world’s most invaded sea. The invasion of alien species results from marine
transportation and aquaculture activities of non-native species. A heavy
maritime traffic is also present in the Marmara Sea, which connects the Black
Sea and Mediterranean Sea. The identification of the invasive species and their
distributions is very prominent in terms of protecting natural habitat and
monitoring the effects of invasive species. In this study, 30 individuals,
morphologically identified as C. gigas, were collected from Bandırma
bay. The genomic DNAs were extracted from each sample’s muscle tissue using
universal salt extraction method. Partial sequences of COI and 16S
Mitochondrial DNA loci of the sample DNAs were obtained for species
identification. The sequences were searched against the database and results
were retrieved from BLAST. All the sequences obtained in this study showed
significant similarity with the C. gigas sequences present in the
database (E=0). The sample sequences resulted in 9 different haplotypes for the
COI locus (hd: 0.5296 and variance: 0.01256±0.112) and 5 different haplotypes
for the 16S rDNA locus (hd: 0.2529, Variance: 0.01076±0.104). The results of
this study provided the first molecular evidence for the presence of non-native
Pacific oyster individuals in the Marmara Sea. |
| first_indexed | 2024-04-10T10:41:58Z |
| format | Article |
| id | doaj.art-2e58e78fdf49440e8be29efd6b841a62 |
| institution | Directory Open Access Journal |
| issn | 2147-9666 |
| language | English |
| last_indexed | 2024-04-10T10:41:58Z |
| publishDate | 2020-06-01 |
| publisher | Adem Yavuz Sönmez |
| record_format | Article |
| series | Marine Science and Technology Bulletin |
| spelling | doaj.art-2e58e78fdf49440e8be29efd6b841a622023-02-15T16:20:29ZengAdem Yavuz SönmezMarine Science and Technology Bulletin2147-96662020-06-0191233110.33714/masteb.6685291306First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, TurkeyEmel ÖZCAN GÖKÇEKSefa ACARLIBilge KARAHANPervin VURALEvren KOBAN BAŞTANLARThe Pacific oyster (Crassostrea gigas) has a very important economic potential for aquaculture, but on the other hand, is among the highly invasive species in the world and within the Mediterranean ecosystem. In the 1960s, C. gigas was brought to Europe for aquaculture in the Mediterranean and Black Sea regions from Japan and Canada. The Turkish waters are the part of the Mediterranean Sea, which is the world’s most invaded sea. The invasion of alien species results from marine transportation and aquaculture activities of non-native species. A heavy maritime traffic is also present in the Marmara Sea, which connects the Black Sea and Mediterranean Sea. The identification of the invasive species and their distributions is very prominent in terms of protecting natural habitat and monitoring the effects of invasive species. In this study, 30 individuals, morphologically identified as C. gigas, were collected from Bandırma bay. The genomic DNAs were extracted from each sample’s muscle tissue using universal salt extraction method. Partial sequences of COI and 16S Mitochondrial DNA loci of the sample DNAs were obtained for species identification. The sequences were searched against the database and results were retrieved from BLAST. All the sequences obtained in this study showed significant similarity with the C. gigas sequences present in the database (E=0). The sample sequences resulted in 9 different haplotypes for the COI locus (hd: 0.5296 and variance: 0.01256±0.112) and 5 different haplotypes for the 16S rDNA locus (hd: 0.2529, Variance: 0.01076±0.104). The results of this study provided the first molecular evidence for the presence of non-native Pacific oyster individuals in the Marmara Sea.https://dergipark.org.tr/en/pub/masteb/issue/50769/668529?publisher=ademyavuzsonmezcrassostrea gigasinvasive speciescoi gene16s mtdnamarmara sea |
| spellingShingle | Emel ÖZCAN GÖKÇEK Sefa ACARLI Bilge KARAHAN Pervin VURAL Evren KOBAN BAŞTANLAR First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey Marine Science and Technology Bulletin crassostrea gigas invasive species coi gene 16s mtdna marmara sea |
| title | First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey |
| title_full | First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey |
| title_fullStr | First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey |
| title_full_unstemmed | First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey |
| title_short | First Molecular Record of the Pacific Oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey |
| title_sort | first molecular record of the pacific oyster crassostrea gigas thunberg 1793 in the marmara sea turkey |
| topic | crassostrea gigas invasive species coi gene 16s mtdna marmara sea |
| url | https://dergipark.org.tr/en/pub/masteb/issue/50769/668529?publisher=ademyavuzsonmez |
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