Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent
A rapid, sensitive and selective spectrophotometric method was developed for determination of sulfathiazole (STHZ) in aqueous solution. The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 10.9) in the presence of potassium period...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Baghdad
2017-03-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| Subjects: | |
| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/112 |
| _version_ | 1818201498060849152 |
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| author | Israa T. Humedy Ismael M. Ali |
| author_facet | Israa T. Humedy Ismael M. Ali |
| author_sort | Israa T. Humedy |
| collection | DOAJ |
| description |
A rapid, sensitive and selective spectrophotometric method was developed for determination of sulfathiazole (STHZ) in aqueous solution. The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 10.9) in the presence of potassium periodate to produce an intense orange colour, soluble in water , stable product and absorbs at 492 nm. Beer's law was in the linear range 2.0-28.0 μg/ml of sulfathiazole, the molar absorptivity, Sandellʼs sensitivity index and detection limit were 1.1437 ×104 liter. mol-1.cm-1,0.0223 μg.cm-2 and 0.1274 μg/ml respectively. The RSD value was 0.75 - 1.12 % depending on the concentration. This method was applied successfully to the determination of sulfathiazole veterinary injection liquid solution (bio prime) with average recovery of not less than 100.38 %.
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| first_indexed | 2024-12-12T02:54:30Z |
| format | Article |
| id | doaj.art-2e5c427d87db4063a78b98fe978d068f |
| institution | Directory Open Access Journal |
| issn | 1609-4042 2521-3407 |
| language | English |
| last_indexed | 2024-12-12T02:54:30Z |
| publishDate | 2017-03-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj.art-2e5c427d87db4063a78b98fe978d068f2022-12-22T00:40:48ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072017-03-01292Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling ReagentIsraa T. HumedyIsmael M. Ali A rapid, sensitive and selective spectrophotometric method was developed for determination of sulfathiazole (STHZ) in aqueous solution. The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 10.9) in the presence of potassium periodate to produce an intense orange colour, soluble in water , stable product and absorbs at 492 nm. Beer's law was in the linear range 2.0-28.0 μg/ml of sulfathiazole, the molar absorptivity, Sandellʼs sensitivity index and detection limit were 1.1437 ×104 liter. mol-1.cm-1,0.0223 μg.cm-2 and 0.1274 μg/ml respectively. The RSD value was 0.75 - 1.12 % depending on the concentration. This method was applied successfully to the determination of sulfathiazole veterinary injection liquid solution (bio prime) with average recovery of not less than 100.38 %. https://jih.uobaghdad.edu.iq/index.php/j/article/view/112Spectrophotometric, sulfathiazole, 2,4-DNPH reagent. |
| spellingShingle | Israa T. Humedy Ismael M. Ali Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent Ibn Al-Haitham Journal for Pure and Applied Sciences Spectrophotometric, sulfathiazole, 2,4-DNPH reagent. |
| title | Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_full | Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_fullStr | Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_full_unstemmed | Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_short | Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_sort | spectrophotometric determination of sulfathiazole using 2 4 a€ dinitrophenylhydrazine as coupling reagent |
| topic | Spectrophotometric, sulfathiazole, 2,4-DNPH reagent. |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/112 |
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