Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions.
Human mesenchymal stem cells (HMSCS) possess three properties of great interest for the development of cell therapies and tissue engineering: multilineage differentiation, immunomodulation, and production of trophic factors. Efficient ex vivo expansion of hMSCs is a challenging requirement for large...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3734034?pdf=render |
| _version_ | 1818039265501642752 |
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| author | Paula J Dolley-Sonneville Lori E Romeo Zara K Melkoumian |
| author_facet | Paula J Dolley-Sonneville Lori E Romeo Zara K Melkoumian |
| author_sort | Paula J Dolley-Sonneville |
| collection | DOAJ |
| description | Human mesenchymal stem cells (HMSCS) possess three properties of great interest for the development of cell therapies and tissue engineering: multilineage differentiation, immunomodulation, and production of trophic factors. Efficient ex vivo expansion of hMSCs is a challenging requirement for large scale production of clinical grade cells. Low-cost, robust, scalable culture methods using chemically defined materials need to be developed to address this need. This study describes the use of a xeno-free synthetic peptide acrylate surface, the Corning® Synthemax® Surface, for culture of hMSCs in serum-free, defined medium. Cell performance on the Corning Synthemax Surface was compared to cells cultured on biological extracellular matrix (ECM) coatings in xeno-free defined medium and in traditional conditions on tissue culture treated (TCT) plastic in fetal bovine serum (FBS) supplemented medium. Our results show successful maintenance of hMSCs on Corning Synthemax Surface for eight passages, with cell expansion rate comparable to cells cultured on ECM and significantly higher than for cells in TCT/FBS condition. Importantly, on the Corning Synthemax Surface, cells maintained elongated, spindle-like morphology, typical hMSC marker profile and in vitro multilineage differentiation potential. We believe the Corning Synthemax Surface, in combination with defined media, provides a complete synthetic, xeno-free, cell culture system for scalable production of hMSCs. |
| first_indexed | 2024-12-10T07:55:53Z |
| format | Article |
| id | doaj.art-2e5c77eadfa848b0aed97a6358e1c72d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:55:53Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2e5c77eadfa848b0aed97a6358e1c72d2022-12-22T01:56:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7026310.1371/journal.pone.0070263Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions.Paula J Dolley-SonnevilleLori E RomeoZara K MelkoumianHuman mesenchymal stem cells (HMSCS) possess three properties of great interest for the development of cell therapies and tissue engineering: multilineage differentiation, immunomodulation, and production of trophic factors. Efficient ex vivo expansion of hMSCs is a challenging requirement for large scale production of clinical grade cells. Low-cost, robust, scalable culture methods using chemically defined materials need to be developed to address this need. This study describes the use of a xeno-free synthetic peptide acrylate surface, the Corning® Synthemax® Surface, for culture of hMSCs in serum-free, defined medium. Cell performance on the Corning Synthemax Surface was compared to cells cultured on biological extracellular matrix (ECM) coatings in xeno-free defined medium and in traditional conditions on tissue culture treated (TCT) plastic in fetal bovine serum (FBS) supplemented medium. Our results show successful maintenance of hMSCs on Corning Synthemax Surface for eight passages, with cell expansion rate comparable to cells cultured on ECM and significantly higher than for cells in TCT/FBS condition. Importantly, on the Corning Synthemax Surface, cells maintained elongated, spindle-like morphology, typical hMSC marker profile and in vitro multilineage differentiation potential. We believe the Corning Synthemax Surface, in combination with defined media, provides a complete synthetic, xeno-free, cell culture system for scalable production of hMSCs.http://europepmc.org/articles/PMC3734034?pdf=render |
| spellingShingle | Paula J Dolley-Sonneville Lori E Romeo Zara K Melkoumian Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions. PLoS ONE |
| title | Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions. |
| title_full | Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions. |
| title_fullStr | Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions. |
| title_full_unstemmed | Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions. |
| title_short | Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions. |
| title_sort | synthetic surface for expansion of human mesenchymal stem cells in xeno free chemically defined culture conditions |
| url | http://europepmc.org/articles/PMC3734034?pdf=render |
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