Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant Growth
Conservation tillage is an extensively used agricultural practice in northern China that alters soil texture and nutrient conditions, causing changes in the soil microbial community. However, how conservation tillage affects rhizosphere and bulk soil fungal communities during plant growth remains un...
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| Format: | Article |
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Frontiers Media S.A.
2017-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01301/full |
| _version_ | 1819049753341591552 |
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| author | Ziting Wang Tong Li Xiaoxia Wen Yang Liu Juan Han Yuncheng Liao Jennifer M. DeBruyn |
| author_facet | Ziting Wang Tong Li Xiaoxia Wen Yang Liu Juan Han Yuncheng Liao Jennifer M. DeBruyn |
| author_sort | Ziting Wang |
| collection | DOAJ |
| description | Conservation tillage is an extensively used agricultural practice in northern China that alters soil texture and nutrient conditions, causing changes in the soil microbial community. However, how conservation tillage affects rhizosphere and bulk soil fungal communities during plant growth remains unclear. The present study investigated the effect of long-term (6 years) conservation (chisel plow, zero) and conventional (plow) tillage during wheat growth on the rhizosphere fungal community, using high-throughput sequencing of the internal transcribed spacer (ITS) gene and quantitative PCR. During tillering, fungal alpha diversity in both rhizosphere and bulk soil were significantly higher under zero tillage compared to other methods. Although tillage had no significant effect during the flowering stage, fungal alpha diversity at this stage was significantly different between rhizosphere and bulk soils, with bulk soil presenting the highest diversity. This was also reflected in the phylogenetic structure of the communities, as rhizosphere soil communities underwent a greater shift from tillering to flowering compared to bulk soil communities. In general, less variation in community structure was observed under zero tillage compared to plow and chisel plow treatments. Changes in the relative abundance of the fungal orders Capnodiales, Pleosporales, and Xylariales contributed the highest to the dissimilarities observed. Structural equation models revealed that the soil fungal communities under the three tillage regimes were likely influenced by the changes in soil properties associated with plant growth. This study suggested that: (1) differences in nutrient resources between rhizosphere and bulk soils can select for different types of fungi thereby increasing community variation during plant growth; (2) tillage can alter fungal communities' variability, with zero tillage promoting more stable communities. This work suggests that long-term changes in tillage regimes may result in unique soil fungal ecology, which might influence other aspects of soil functioning (e.g., decomposition). |
| first_indexed | 2024-12-21T11:37:10Z |
| format | Article |
| id | doaj.art-2e5dc36e417449fe95853f9bc54fde3c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T11:37:10Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2e5dc36e417449fe95853f9bc54fde3c2022-12-21T19:05:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-07-01810.3389/fmicb.2017.01301280641Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant GrowthZiting Wang0Tong Li1Xiaoxia Wen2Yang Liu3Juan Han4Yuncheng Liao5Jennifer M. DeBruyn6College of Agronomy, Northwest A&F UniversityYangling, ChinaCollege of Agronomy, Northwest A&F UniversityYangling, ChinaCollege of Agronomy, Northwest A&F UniversityYangling, ChinaCollege of Agronomy, Northwest A&F UniversityYangling, ChinaCollege of Agronomy, Northwest A&F UniversityYangling, ChinaCollege of Agronomy, Northwest A&F UniversityYangling, ChinaDepartment of Biosystems Engineering and Soil Science, The University of TennesseeKnoxville, TN, United StatesConservation tillage is an extensively used agricultural practice in northern China that alters soil texture and nutrient conditions, causing changes in the soil microbial community. However, how conservation tillage affects rhizosphere and bulk soil fungal communities during plant growth remains unclear. The present study investigated the effect of long-term (6 years) conservation (chisel plow, zero) and conventional (plow) tillage during wheat growth on the rhizosphere fungal community, using high-throughput sequencing of the internal transcribed spacer (ITS) gene and quantitative PCR. During tillering, fungal alpha diversity in both rhizosphere and bulk soil were significantly higher under zero tillage compared to other methods. Although tillage had no significant effect during the flowering stage, fungal alpha diversity at this stage was significantly different between rhizosphere and bulk soils, with bulk soil presenting the highest diversity. This was also reflected in the phylogenetic structure of the communities, as rhizosphere soil communities underwent a greater shift from tillering to flowering compared to bulk soil communities. In general, less variation in community structure was observed under zero tillage compared to plow and chisel plow treatments. Changes in the relative abundance of the fungal orders Capnodiales, Pleosporales, and Xylariales contributed the highest to the dissimilarities observed. Structural equation models revealed that the soil fungal communities under the three tillage regimes were likely influenced by the changes in soil properties associated with plant growth. This study suggested that: (1) differences in nutrient resources between rhizosphere and bulk soils can select for different types of fungi thereby increasing community variation during plant growth; (2) tillage can alter fungal communities' variability, with zero tillage promoting more stable communities. This work suggests that long-term changes in tillage regimes may result in unique soil fungal ecology, which might influence other aspects of soil functioning (e.g., decomposition).http://journal.frontiersin.org/article/10.3389/fmicb.2017.01301/fullconservation tillagerhizosphere soilfungal communityplant growthstructural equation model |
| spellingShingle | Ziting Wang Tong Li Xiaoxia Wen Yang Liu Juan Han Yuncheng Liao Jennifer M. DeBruyn Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant Growth Frontiers in Microbiology conservation tillage rhizosphere soil fungal community plant growth structural equation model |
| title | Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant Growth |
| title_full | Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant Growth |
| title_fullStr | Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant Growth |
| title_full_unstemmed | Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant Growth |
| title_short | Fungal Communities in Rhizosphere Soil under Conservation Tillage Shift in Response to Plant Growth |
| title_sort | fungal communities in rhizosphere soil under conservation tillage shift in response to plant growth |
| topic | conservation tillage rhizosphere soil fungal community plant growth structural equation model |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01301/full |
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