Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases
Vitamin D plays a role in anti-inflammatory processes, and the alteration of its metabolism is associated with the inflammatory processes of pancreatitis. This study was performed to evaluate the expression of the vitamin D receptor (VDR) and the two major enzymes that regulate vitamin D metabolism,...
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1265203/full |
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| author | Dohee Lee Sanggu Kim Yoonhoi Koo Yeon Chae Juwon Wang Soochong Kim Taesik Yun Mhan-Pyo Yang Byeong-Teck Kang Hakhyun Kim |
| author_facet | Dohee Lee Sanggu Kim Yoonhoi Koo Yeon Chae Juwon Wang Soochong Kim Taesik Yun Mhan-Pyo Yang Byeong-Teck Kang Hakhyun Kim |
| author_sort | Dohee Lee |
| collection | DOAJ |
| description | Vitamin D plays a role in anti-inflammatory processes, and the alteration of its metabolism is associated with the inflammatory processes of pancreatitis. This study was performed to evaluate the expression of the vitamin D receptor (VDR) and the two major enzymes that regulate vitamin D metabolism, 1α-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24A1), in the canine pancreas and to compare their degrees of immunoreactivity between normal and inflamed pancreases. Five normal and inflamed pancreatic tissues each were obtained from six dogs. The expression of VDR, CYP24A1, and CYP27B1 were determined immunohistochemically, and the degree of immunostaining was assessed semiquantitatively. The VDR was expressed in the ducts, acini, and islets of Langerhans of normal pancreases and in the ducts and acini of inflamed ones. There was a significant difference in the immunoreactivity score for VDR in the islets of Langerhans between normal (median, 3 [interquartile range, 2–7.5] score) and inflamed pancreatic tissues (0 [0–0.5] score, p = 0.03). CYP24A1 was expressed in the ducts and islets of Langerhans in both normal and inflamed pancreases, whereas CYP27B1 was expressed in the ducts and acini in only some normal and inflamed pancreatic tissues. This study showed that VDR expression decreased in inflamed pancreases and demonstrated CYP24A1 and CYP27B1 expression in the canine pancreas for the first time. These findings indicate that the pancreas could regulate the metabolism and biological activity of vitamin D and suggest that a decrease in these might be related to the pathophysiology of pancreatitis. |
| first_indexed | 2024-03-11T22:56:24Z |
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| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-11T22:56:24Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-2e5f5972f14a43fcbd6e97928fb7c3232023-09-21T15:29:21ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-09-011010.3389/fvets.2023.12652031265203Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreasesDohee Lee0Sanggu Kim1Yoonhoi Koo2Yeon Chae3Juwon Wang4Soochong Kim5Taesik Yun6Mhan-Pyo Yang7Byeong-Teck Kang8Hakhyun Kim9Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Pathology and Platelet Signaling, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Pathology and Platelet Signaling, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaVitamin D plays a role in anti-inflammatory processes, and the alteration of its metabolism is associated with the inflammatory processes of pancreatitis. This study was performed to evaluate the expression of the vitamin D receptor (VDR) and the two major enzymes that regulate vitamin D metabolism, 1α-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24A1), in the canine pancreas and to compare their degrees of immunoreactivity between normal and inflamed pancreases. Five normal and inflamed pancreatic tissues each were obtained from six dogs. The expression of VDR, CYP24A1, and CYP27B1 were determined immunohistochemically, and the degree of immunostaining was assessed semiquantitatively. The VDR was expressed in the ducts, acini, and islets of Langerhans of normal pancreases and in the ducts and acini of inflamed ones. There was a significant difference in the immunoreactivity score for VDR in the islets of Langerhans between normal (median, 3 [interquartile range, 2–7.5] score) and inflamed pancreatic tissues (0 [0–0.5] score, p = 0.03). CYP24A1 was expressed in the ducts and islets of Langerhans in both normal and inflamed pancreases, whereas CYP27B1 was expressed in the ducts and acini in only some normal and inflamed pancreatic tissues. This study showed that VDR expression decreased in inflamed pancreases and demonstrated CYP24A1 and CYP27B1 expression in the canine pancreas for the first time. These findings indicate that the pancreas could regulate the metabolism and biological activity of vitamin D and suggest that a decrease in these might be related to the pathophysiology of pancreatitis.https://www.frontiersin.org/articles/10.3389/fvets.2023.1265203/fullCYP24A1CYP27B1dogimmunohistochemistrypancreatic tissuepancreatitis |
| spellingShingle | Dohee Lee Sanggu Kim Yoonhoi Koo Yeon Chae Juwon Wang Soochong Kim Taesik Yun Mhan-Pyo Yang Byeong-Teck Kang Hakhyun Kim Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases Frontiers in Veterinary Science CYP24A1 CYP27B1 dog immunohistochemistry pancreatic tissue pancreatitis |
| title | Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases |
| title_full | Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases |
| title_fullStr | Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases |
| title_full_unstemmed | Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases |
| title_short | Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases |
| title_sort | expression of vitamin d receptor cyp24a1 and cyp27b1 in normal and inflamed canine pancreases |
| topic | CYP24A1 CYP27B1 dog immunohistochemistry pancreatic tissue pancreatitis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1265203/full |
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