Imbalance of endogenous prostanoids in moderate-to-severe asthma
Background: Inhalation studies suggested “protective” roles of exogenous prostaglandin E2, but the clinical relevance of endogenous prostanoids in asthma is poorly known. The objective of this study is to measure sputum levels of prostanoids in asthmatic patients to correlate with clinical indices....
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Allergology International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893016300843 |
| _version_ | 1818606681152552960 |
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| author | Masaya Takemura Akio Niimi Hisako Matsumoto Tetsuya Ueda Masafumi Yamaguchi Hirofumi Matsuoka Makiko Jinnai Kian Fan Chung Michiaki Mishima |
| author_facet | Masaya Takemura Akio Niimi Hisako Matsumoto Tetsuya Ueda Masafumi Yamaguchi Hirofumi Matsuoka Makiko Jinnai Kian Fan Chung Michiaki Mishima |
| author_sort | Masaya Takemura |
| collection | DOAJ |
| description | Background: Inhalation studies suggested “protective” roles of exogenous prostaglandin E2, but the clinical relevance of endogenous prostanoids in asthma is poorly known. The objective of this study is to measure sputum levels of prostanoids in asthmatic patients to correlate with clinical indices.
Methods: Mild (n = 41) or moderate-to-severe (19) asthmatics and 27 normal controls were examined for pulmonary function (FEV1 and mid-forced expiratory flow), sputum cell differentials, and sputum levels of prostaglandins D2, E2, F2α, and thromboxane B2 measured by sandwich enzyme immunoassay.
Results: Each prostanoid level did not differ among the three groups. Sputum number of bronchial epithelial cells was greater in moderate-to-severe asthmatics than in the other two groups, suggesting epithelial desquamation. Levels of prostaglandin F2α, D2, and thromboxane B2 positively correlated with the severity of airflow obstruction in the 60 asthmatic patients, whereas prostaglandin E2 levels were unrelated to pulmonary function. The ratio of combined “contractile” prostanoids (prostaglandin D2/prostaglandin F2α/thromboxane B2) to prostaglandin E2 was 2.5-fold greater in moderate-to-severe asthmatics than in controls (p = 0.001) or in mild asthmatics (p = 0.0002) but did not differ between the latter two groups. In the two asthmatic groups combined, this ratio positively correlated with the sputum number of epithelial cells. The combined “contractile” prostanoids levels positively correlated with prostaglandin E2 levels in controls and in mild asthmatics but not in moderate-to-severe asthmatics.
Conclusions: An imbalance in production, breakdown, or both between prostaglandin E2 and other prostanoids possibly due to epithelial damage may be involved in the pathogenesis of moderate-to-severe asthma. |
| first_indexed | 2024-12-16T14:14:43Z |
| format | Article |
| id | doaj.art-2e626350452c432190e866ad6e78e318 |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-16T14:14:43Z |
| publishDate | 2017-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-2e626350452c432190e866ad6e78e3182022-12-21T22:28:39ZengElsevierAllergology International1323-89302017-01-01661838810.1016/j.alit.2016.05.013Imbalance of endogenous prostanoids in moderate-to-severe asthmaMasaya Takemura0Akio Niimi1Hisako Matsumoto2Tetsuya Ueda3Masafumi Yamaguchi4Hirofumi Matsuoka5Makiko Jinnai6Kian Fan Chung7Michiaki Mishima8Department of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanThoracic Medicine, National Heart & Lung Institute, Imperial College, London, UKDepartment of Respiratory Medicine, Kyoto University, Kyoto, JapanBackground: Inhalation studies suggested “protective” roles of exogenous prostaglandin E2, but the clinical relevance of endogenous prostanoids in asthma is poorly known. The objective of this study is to measure sputum levels of prostanoids in asthmatic patients to correlate with clinical indices. Methods: Mild (n = 41) or moderate-to-severe (19) asthmatics and 27 normal controls were examined for pulmonary function (FEV1 and mid-forced expiratory flow), sputum cell differentials, and sputum levels of prostaglandins D2, E2, F2α, and thromboxane B2 measured by sandwich enzyme immunoassay. Results: Each prostanoid level did not differ among the three groups. Sputum number of bronchial epithelial cells was greater in moderate-to-severe asthmatics than in the other two groups, suggesting epithelial desquamation. Levels of prostaglandin F2α, D2, and thromboxane B2 positively correlated with the severity of airflow obstruction in the 60 asthmatic patients, whereas prostaglandin E2 levels were unrelated to pulmonary function. The ratio of combined “contractile” prostanoids (prostaglandin D2/prostaglandin F2α/thromboxane B2) to prostaglandin E2 was 2.5-fold greater in moderate-to-severe asthmatics than in controls (p = 0.001) or in mild asthmatics (p = 0.0002) but did not differ between the latter two groups. In the two asthmatic groups combined, this ratio positively correlated with the sputum number of epithelial cells. The combined “contractile” prostanoids levels positively correlated with prostaglandin E2 levels in controls and in mild asthmatics but not in moderate-to-severe asthmatics. Conclusions: An imbalance in production, breakdown, or both between prostaglandin E2 and other prostanoids possibly due to epithelial damage may be involved in the pathogenesis of moderate-to-severe asthma.http://www.sciencedirect.com/science/article/pii/S1323893016300843AsthmaEpithelial damageInduced sputumProstaglandinThromboxane |
| spellingShingle | Masaya Takemura Akio Niimi Hisako Matsumoto Tetsuya Ueda Masafumi Yamaguchi Hirofumi Matsuoka Makiko Jinnai Kian Fan Chung Michiaki Mishima Imbalance of endogenous prostanoids in moderate-to-severe asthma Allergology International Asthma Epithelial damage Induced sputum Prostaglandin Thromboxane |
| title | Imbalance of endogenous prostanoids in moderate-to-severe asthma |
| title_full | Imbalance of endogenous prostanoids in moderate-to-severe asthma |
| title_fullStr | Imbalance of endogenous prostanoids in moderate-to-severe asthma |
| title_full_unstemmed | Imbalance of endogenous prostanoids in moderate-to-severe asthma |
| title_short | Imbalance of endogenous prostanoids in moderate-to-severe asthma |
| title_sort | imbalance of endogenous prostanoids in moderate to severe asthma |
| topic | Asthma Epithelial damage Induced sputum Prostaglandin Thromboxane |
| url | http://www.sciencedirect.com/science/article/pii/S1323893016300843 |
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