Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study
Nanoformulation-based combinational drug delivery systems are well known to overcome drug resistance in cancer management. Among them, nanoemulsions are well-known and thermodynamically stable drug delivery systems suitable for carrying hydrophobic drugs and phytoconstituents to tackle drug-resistan...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1020602/full |
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| author | Harneetpal Kaur Kirandeep Kaur Atamjit Singh Neena Bedi Balbir Singh Mansour S. Alturki Mohammed F. Aldawsari Atiah H. Almalki Atiah H. Almalki Shafiul Haque Hae-Jeung Lee Dharmendra K. Yadav Saroj Arora |
| author_facet | Harneetpal Kaur Kirandeep Kaur Atamjit Singh Neena Bedi Balbir Singh Mansour S. Alturki Mohammed F. Aldawsari Atiah H. Almalki Atiah H. Almalki Shafiul Haque Hae-Jeung Lee Dharmendra K. Yadav Saroj Arora |
| author_sort | Harneetpal Kaur |
| collection | DOAJ |
| description | Nanoformulation-based combinational drug delivery systems are well known to overcome drug resistance in cancer management. Among them, nanoemulsions are well-known and thermodynamically stable drug delivery systems suitable for carrying hydrophobic drugs and phytoconstituents to tackle drug-resistant cancers. In the present study, we have investigated the effect of paclitaxel in combination with erucin (natural isothiocyanate isolated from the seeds of Eruca sativa) loaded in the frankincense oil-based nanoemulsion formulation. The choice of frankincense oil for the current study was based on reported research investigations stating its magnificient therapeutic potential against breast cancer. Optimized nanoemulsion of paclitaxel (PTX) and erucin (ER) combination (EPNE) provided sustained release and exhibited enhanced cytotoxicity towards human epithelial breast cancer cells (T-47D) as compared to individual ER and PTX. EPNE was further assessed for its antitumor activity in the 7,12-dimethylbenz(a)anthracene (DMBA)-induced breast cancer mice model. EPNE significantly decreased the levels of hepatic and renal parameters along with oxidative stress in breast cancer mice. Furthermore, EPNE also showed decreased levels of inflammatory cytokines TNF-α, IL-6. Histopathological examinations revealed restoration of the tumorous breast to normal tissues in EPNE-treated breast cancer mice. Therefore, EPNE can act as a viable lead and therapeutic option for drug-resistant breast cancer. |
| first_indexed | 2024-04-11T19:34:52Z |
| format | Article |
| id | doaj.art-2e63a133fb274d47a419e2b81a40f9d4 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T19:34:52Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-2e63a133fb274d47a419e2b81a40f9d42022-12-22T04:06:53ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-10-011310.3389/fphar.2022.10206021020602Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo studyHarneetpal Kaur0Kirandeep Kaur1Atamjit Singh2Neena Bedi3Balbir Singh4Mansour S. Alturki5Mohammed F. Aldawsari6Atiah H. Almalki7Atiah H. Almalki8Shafiul Haque9Hae-Jeung Lee10Dharmendra K. Yadav11Saroj Arora12Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, IndiaDepartment of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab, IndiaDepartment of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab, IndiaDepartment of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab, IndiaDepartment of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab, IndiaDepartment of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi ArabiaAddiction and Neuroscience Research Unit, College of Pharmacy, Taif University, Taif, Saudi ArabiaResearch and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan, Saudi ArabiaDepartment of Food and Nutrition, College of Bionano Technology, Gachon University, Seongnam-si, Gyeonggi-do, South KoreaCollege of Pharmacy, Gachon University of Medicine and Science, Incheon City, KoreaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, IndiaNanoformulation-based combinational drug delivery systems are well known to overcome drug resistance in cancer management. Among them, nanoemulsions are well-known and thermodynamically stable drug delivery systems suitable for carrying hydrophobic drugs and phytoconstituents to tackle drug-resistant cancers. In the present study, we have investigated the effect of paclitaxel in combination with erucin (natural isothiocyanate isolated from the seeds of Eruca sativa) loaded in the frankincense oil-based nanoemulsion formulation. The choice of frankincense oil for the current study was based on reported research investigations stating its magnificient therapeutic potential against breast cancer. Optimized nanoemulsion of paclitaxel (PTX) and erucin (ER) combination (EPNE) provided sustained release and exhibited enhanced cytotoxicity towards human epithelial breast cancer cells (T-47D) as compared to individual ER and PTX. EPNE was further assessed for its antitumor activity in the 7,12-dimethylbenz(a)anthracene (DMBA)-induced breast cancer mice model. EPNE significantly decreased the levels of hepatic and renal parameters along with oxidative stress in breast cancer mice. Furthermore, EPNE also showed decreased levels of inflammatory cytokines TNF-α, IL-6. Histopathological examinations revealed restoration of the tumorous breast to normal tissues in EPNE-treated breast cancer mice. Therefore, EPNE can act as a viable lead and therapeutic option for drug-resistant breast cancer.https://www.frontiersin.org/articles/10.3389/fphar.2022.1020602/fullerucinpaclitaxelsynergismfrankincense oilnanoemulsionbreast cancer |
| spellingShingle | Harneetpal Kaur Kirandeep Kaur Atamjit Singh Neena Bedi Balbir Singh Mansour S. Alturki Mohammed F. Aldawsari Atiah H. Almalki Atiah H. Almalki Shafiul Haque Hae-Jeung Lee Dharmendra K. Yadav Saroj Arora Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study Frontiers in Pharmacology erucin paclitaxel synergism frankincense oil nanoemulsion breast cancer |
| title | Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study |
| title_full | Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study |
| title_fullStr | Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study |
| title_full_unstemmed | Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study |
| title_short | Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study |
| title_sort | frankincense oil loaded nanoemulsion formulation of paclitaxel and erucin a synergistic combination for ameliorating drug resistance in breast cancer in vitro and in vivo study |
| topic | erucin paclitaxel synergism frankincense oil nanoemulsion breast cancer |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1020602/full |
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