Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A Modification
Host translation is generally modulated by viral infection, including duck hepatitis A virus (DHAV) infection. Previously, we reported that cellular protein synthesis in a cell model of duck embryo fibroblasts is significantly inhibited by DHAV infection but not viral proteins, suggesting that an im...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.839677/full |
| _version_ | 1828777351915241472 |
|---|---|
| author | Liping Wu Weili Quan Yi Zhang Mingshu Wang Xumin Ou Sai Mao Di Sun Qiao Yang Ying Wu Yaxun Wei Renyong Jia Shun Chen Dekang Zhu Mafeng Liu Xinxin Zhao Shaqiu Zhang Juan Huang Qun Gao Bin Tian Anchun Cheng |
| author_facet | Liping Wu Weili Quan Yi Zhang Mingshu Wang Xumin Ou Sai Mao Di Sun Qiao Yang Ying Wu Yaxun Wei Renyong Jia Shun Chen Dekang Zhu Mafeng Liu Xinxin Zhao Shaqiu Zhang Juan Huang Qun Gao Bin Tian Anchun Cheng |
| author_sort | Liping Wu |
| collection | DOAJ |
| description | Host translation is generally modulated by viral infection, including duck hepatitis A virus (DHAV) infection. Previously, we reported that cellular protein synthesis in a cell model of duck embryo fibroblasts is significantly inhibited by DHAV infection but not viral proteins, suggesting that an important viral-host interaction occurs at the translational level. In this study, we aim to further understand the impact of DHAV virulence on cellular N6-methyladenosine (m6A) modification, which is essential to a wide variety of RNA biological processes, such as mRNA stabilization and translation. Using m6A antibody-based immunoprecipitation, m6A-seq, and LC–MS/MS, we observed that m6A-modified mRNA exists in both virulent and attenuated DHAV-infected duckling livers. Importantly, m6A levels in mRNA were much higher in attenuated DHAV-infected livers compared with virulent DHAV-infected livers, suggesting virulence-dependent regulation of m6A modification. Analysis of modification motifs indicated that GAAGAAG is the most enriched motif. Combined m6A-seq and RNA-seq data analysis indicated a generally positive correlation between m6A and mRNA expression levels in DHAV-infected duckling livers. GO analysis of genes with decreased or increased m6A levels showed that these genes were enriched in various terms, including oxidation–reduction processes and antiviral immune responses. Collectively, our work reveals DHAV virulence-dependent coordination between m6A modification and mRNA expression in duckling livers. |
| first_indexed | 2024-12-11T16:18:51Z |
| format | Article |
| id | doaj.art-2e63dcb64152489ea22cf2e26e8fb1f4 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T16:18:51Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-2e63dcb64152489ea22cf2e26e8fb1f42022-12-22T00:58:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-06-011310.3389/fimmu.2022.839677839677Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A ModificationLiping Wu0Weili Quan1Yi Zhang2Mingshu Wang3Xumin Ou4Sai Mao5Di Sun6Qiao Yang7Ying Wu8Yaxun Wei9Renyong Jia10Shun Chen11Dekang Zhu12Mafeng Liu13Xinxin Zhao14Shaqiu Zhang15Juan Huang16Qun Gao17Bin Tian18Anchun Cheng19Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaABLife BioBigData Institute, Wuhan, ChinaABLife BioBigData Institute, Wuhan, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCenter for Genome Analysis, ABLife, Inc., Wuhan, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaHost translation is generally modulated by viral infection, including duck hepatitis A virus (DHAV) infection. Previously, we reported that cellular protein synthesis in a cell model of duck embryo fibroblasts is significantly inhibited by DHAV infection but not viral proteins, suggesting that an important viral-host interaction occurs at the translational level. In this study, we aim to further understand the impact of DHAV virulence on cellular N6-methyladenosine (m6A) modification, which is essential to a wide variety of RNA biological processes, such as mRNA stabilization and translation. Using m6A antibody-based immunoprecipitation, m6A-seq, and LC–MS/MS, we observed that m6A-modified mRNA exists in both virulent and attenuated DHAV-infected duckling livers. Importantly, m6A levels in mRNA were much higher in attenuated DHAV-infected livers compared with virulent DHAV-infected livers, suggesting virulence-dependent regulation of m6A modification. Analysis of modification motifs indicated that GAAGAAG is the most enriched motif. Combined m6A-seq and RNA-seq data analysis indicated a generally positive correlation between m6A and mRNA expression levels in DHAV-infected duckling livers. GO analysis of genes with decreased or increased m6A levels showed that these genes were enriched in various terms, including oxidation–reduction processes and antiviral immune responses. Collectively, our work reveals DHAV virulence-dependent coordination between m6A modification and mRNA expression in duckling livers.https://www.frontiersin.org/articles/10.3389/fimmu.2022.839677/fullduck hepatitis A virusm6A modificationliverduck viral hepatitisvirulence |
| spellingShingle | Liping Wu Weili Quan Yi Zhang Mingshu Wang Xumin Ou Sai Mao Di Sun Qiao Yang Ying Wu Yaxun Wei Renyong Jia Shun Chen Dekang Zhu Mafeng Liu Xinxin Zhao Shaqiu Zhang Juan Huang Qun Gao Bin Tian Anchun Cheng Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A Modification Frontiers in Immunology duck hepatitis A virus m6A modification liver duck viral hepatitis virulence |
| title | Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A Modification |
| title_full | Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A Modification |
| title_fullStr | Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A Modification |
| title_full_unstemmed | Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A Modification |
| title_short | Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A Modification |
| title_sort | attenuated duck hepatitis a virus infection is associated with high mrna maintenance in duckling liver via m6a modification |
| topic | duck hepatitis A virus m6A modification liver duck viral hepatitis virulence |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.839677/full |
| work_keys_str_mv | AT lipingwu attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT weiliquan attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT yizhang attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT mingshuwang attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT xuminou attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT saimao attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT disun attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT qiaoyang attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT yingwu attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT yaxunwei attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT renyongjia attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT shunchen attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT dekangzhu attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT mafengliu attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT xinxinzhao attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT shaqiuzhang attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT juanhuang attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT qungao attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT bintian attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification AT anchuncheng attenuatedduckhepatitisavirusinfectionisassociatedwithhighmrnamaintenanceinducklingliverviam6amodification |