Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens
Sensitization with conceptus antigens has been shown to be useful for improving reproductive performance facilitating maternal acceptance of an allogeneic embryo through the induction of cytokines and immunoregulatory cells in the uterine microenvironment. As FOXP3, IDO, IL10 and CSF1 in the uterus...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2020-09-01
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| Series: | Bioscience Journal |
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| Online Access: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/48218 |
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| author | Talita Vieta Junqueira Nadia Simarro Fagundes Marcelo José Barbosa Silva João Paulo Elsen Saut Marcelo Emílio Beletti |
| author_facet | Talita Vieta Junqueira Nadia Simarro Fagundes Marcelo José Barbosa Silva João Paulo Elsen Saut Marcelo Emílio Beletti |
| author_sort | Talita Vieta Junqueira |
| collection | DOAJ |
| description | Sensitization with conceptus antigens has been shown to be useful for improving reproductive performance facilitating maternal acceptance of an allogeneic embryo through the induction of cytokines and immunoregulatory cells in the uterine microenvironment. As FOXP3, IDO, IL10 and CSF1 in the uterus are important on the recognition and development of embryos during early pregnancy, this study aimed to determine whether simultaneous or isolated administration of paternal (semen) and maternal (PBMCs) antigens in the uterus of cow, on the day of estrus, influence the gene expression of these cytokines. Forty crossbred cows were divided into four treatments: T0: Control; T1: Semen; T2: PBMCs (peripheral blood mononuclear cells) from another cow and T3: PBMCs+Semen. Antigens were administered into the uterine body on the estrus day (D0). Uterine biopsies designed for molecular analysis of gene expression were collected in vivo seven (D7) and fourteen (D14) days after immunostimulation. Transcripts from FOXP3, IDO, IL-10 and CSF-1 were detected in all RNA samples extracted from uterine biopsies. The semiquantitative analysis showed that none of the treatments caused significant increase in the expression of these genes. Furthermore, on D14 all treatments led to a decline in the number of CSF-1 transcripts; moreover, treatment with PBMCs+Semen also led to a drop in the abundance of IL-10 transcripts. Such results suggest that isolated or simultaneous administration of both antigens would not increase maternal tolerance to embryo alloantigens, nor would it create favorable conditions to its growth and pre-implantation development, at least regarding the effects mediated by these genes on D7 and D14 of the estrous cycle. |
| first_indexed | 2024-12-16T17:26:51Z |
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| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-16T17:26:51Z |
| publishDate | 2020-09-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-2e64be010c8d4d31bd6f01212d584f202022-12-21T22:23:02ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632020-09-0136610.14393/BJ-v36n6a2020-4821848218Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigensTalita Vieta Junqueira0Nadia Simarro Fagundes1Marcelo José Barbosa Silva2João Paulo Elsen SautMarcelo Emílio Beletti3Universidade Federal de UberlândiaUniversidade Federal de UberlândiaUniversidade Federal de UberlândiaUniversidade Federal de UberlândiaSensitization with conceptus antigens has been shown to be useful for improving reproductive performance facilitating maternal acceptance of an allogeneic embryo through the induction of cytokines and immunoregulatory cells in the uterine microenvironment. As FOXP3, IDO, IL10 and CSF1 in the uterus are important on the recognition and development of embryos during early pregnancy, this study aimed to determine whether simultaneous or isolated administration of paternal (semen) and maternal (PBMCs) antigens in the uterus of cow, on the day of estrus, influence the gene expression of these cytokines. Forty crossbred cows were divided into four treatments: T0: Control; T1: Semen; T2: PBMCs (peripheral blood mononuclear cells) from another cow and T3: PBMCs+Semen. Antigens were administered into the uterine body on the estrus day (D0). Uterine biopsies designed for molecular analysis of gene expression were collected in vivo seven (D7) and fourteen (D14) days after immunostimulation. Transcripts from FOXP3, IDO, IL-10 and CSF-1 were detected in all RNA samples extracted from uterine biopsies. The semiquantitative analysis showed that none of the treatments caused significant increase in the expression of these genes. Furthermore, on D14 all treatments led to a decline in the number of CSF-1 transcripts; moreover, treatment with PBMCs+Semen also led to a drop in the abundance of IL-10 transcripts. Such results suggest that isolated or simultaneous administration of both antigens would not increase maternal tolerance to embryo alloantigens, nor would it create favorable conditions to its growth and pre-implantation development, at least regarding the effects mediated by these genes on D7 and D14 of the estrous cycle.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/48218bos taurus.pregnancy.immunologic induction.peripheral blood mononuclear cells.semen. |
| spellingShingle | Talita Vieta Junqueira Nadia Simarro Fagundes Marcelo José Barbosa Silva João Paulo Elsen Saut Marcelo Emílio Beletti Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens Bioscience Journal bos taurus. pregnancy. immunologic induction. peripheral blood mononuclear cells. semen. |
| title | Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens |
| title_full | Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens |
| title_fullStr | Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens |
| title_full_unstemmed | Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens |
| title_short | Gene expression of FOXP3, indoleamine 2,3-dioxygenase (IDO), IL10 and CSF1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens |
| title_sort | gene expression of foxp3 indoleamine 2 3 dioxygenase ido il10 and csf1 in the uterus of cows that received an intrauterine infusion of maternal and paternal antigens |
| topic | bos taurus. pregnancy. immunologic induction. peripheral blood mononuclear cells. semen. |
| url | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/48218 |
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