Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress Tolerance
Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. S...
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MDPI AG
2020-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/6/2177 |
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| author | Bo Li Jia-Cheng Zheng Ting-Ting Wang Dong-Hong Min Wen-Liang Wei Jun Chen Yong-Bin Zhou Ming Chen Zhao-Shi Xu You-Zhi Ma |
| author_facet | Bo Li Jia-Cheng Zheng Ting-Ting Wang Dong-Hong Min Wen-Liang Wei Jun Chen Yong-Bin Zhou Ming Chen Zhao-Shi Xu You-Zhi Ma |
| author_sort | Bo Li |
| collection | DOAJ |
| description | Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (<i>GmVOZs</i>) have been reported to exist in the soybean (<i>Glycine max</i>) genome. In spite of this, little information is currently available regarding <i>GmVOZs.</i> In this study, <i>GmVOZs</i> were cloned and characterized. <i>GmVOZ</i> genes encode proteins possessing transcriptional activation activity in yeast cells. <i>GmVOZ1E</i>, <i>GmVOZ2B</i>, and <i>GmVOZ2D</i> gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. <i>GmVOZs</i> displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, <i>GmVOZ1G</i> showed a significantly induced expression in response to all stress treatments. Overexpression of <i>GmVOZ1G</i> in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing <i>GmVOZ1G</i> were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, <i>GmVOZ1G</i> positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress. |
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| language | English |
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| spelling | doaj.art-2e6588cddc9045e7a2231ec5ef0bb9c82022-12-22T03:15:36ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-03-01216217710.3390/ijms21062177ijms21062177Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress ToleranceBo Li0Jia-Cheng Zheng1Ting-Ting Wang2Dong-Hong Min3Wen-Liang Wei4Jun Chen5Yong-Bin Zhou6Ming Chen7Zhao-Shi Xu8You-Zhi Ma9Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, ChinaAnhui Science and Technology University, Fengyang 233100, ChinaCollege of Agriculture, Yangtze University; Hubei Collaborative Innovation Center for Grain Industry; Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434025, ChinaCollege of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, Shaanxi 712100, ChinaCollege of Agriculture, Yangtze University; Hubei Collaborative Innovation Center for Grain Industry; Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434025, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, ChinaVascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (<i>GmVOZs</i>) have been reported to exist in the soybean (<i>Glycine max</i>) genome. In spite of this, little information is currently available regarding <i>GmVOZs.</i> In this study, <i>GmVOZs</i> were cloned and characterized. <i>GmVOZ</i> genes encode proteins possessing transcriptional activation activity in yeast cells. <i>GmVOZ1E</i>, <i>GmVOZ2B</i>, and <i>GmVOZ2D</i> gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. <i>GmVOZs</i> displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, <i>GmVOZ1G</i> showed a significantly induced expression in response to all stress treatments. Overexpression of <i>GmVOZ1G</i> in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing <i>GmVOZ1G</i> were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, <i>GmVOZ1G</i> positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.https://www.mdpi.com/1422-0067/21/6/2177soybeanvoz transcription factorexpression characterizationstress responsedrought and salt tolerance |
| spellingShingle | Bo Li Jia-Cheng Zheng Ting-Ting Wang Dong-Hong Min Wen-Liang Wei Jun Chen Yong-Bin Zhou Ming Chen Zhao-Shi Xu You-Zhi Ma Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress Tolerance International Journal of Molecular Sciences soybean voz transcription factor expression characterization stress response drought and salt tolerance |
| title | Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress Tolerance |
| title_full | Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress Tolerance |
| title_fullStr | Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress Tolerance |
| title_full_unstemmed | Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress Tolerance |
| title_short | Expression Analyses of Soybean VOZ Transcription Factors and the Role of <i>GmVOZ1G</i> in Drought and Salt Stress Tolerance |
| title_sort | expression analyses of soybean voz transcription factors and the role of i gmvoz1g i in drought and salt stress tolerance |
| topic | soybean voz transcription factor expression characterization stress response drought and salt tolerance |
| url | https://www.mdpi.com/1422-0067/21/6/2177 |
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