Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles
Wharton’s jelly-derived mesenchymal stem cell (WJ-MSC)-derived exosomes contain a diverse cargo and exhibit remarkable biological activity, rendering them suitable for regenerative and immune-modulating functions. However, the quantity of secretion is insufficient. A large body of prior work has inv...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/22/16460 |
| _version_ | 1797458968425005056 |
|---|---|
| author | SangRok Park SeJong Kim KyungMin Lim YeoKyung Shin Kwonwoo Song Geun-Ho Kang Dae Young Kim Hang-Cheol Shin Ssang-Goo Cho |
| author_facet | SangRok Park SeJong Kim KyungMin Lim YeoKyung Shin Kwonwoo Song Geun-Ho Kang Dae Young Kim Hang-Cheol Shin Ssang-Goo Cho |
| author_sort | SangRok Park |
| collection | DOAJ |
| description | Wharton’s jelly-derived mesenchymal stem cell (WJ-MSC)-derived exosomes contain a diverse cargo and exhibit remarkable biological activity, rendering them suitable for regenerative and immune-modulating functions. However, the quantity of secretion is insufficient. A large body of prior work has investigated the use of various growth factors to enhance MSC-derived exosome production. In this study, we evaluated the utilization of thermostable basic fibroblast growth factor (TS-bFGF) with MSC culture and exosome production. MSCs cultured with TS-bFGF displayed superior proliferation, as evidenced by cell cycle analysis, compared with wild-type bFGF (WT-bFGF). Stemness was assessed through mRNA expression level and colony-forming unit (CFU) assays. Furthermore, nanoparticle tracking analysis (NTA) measurements revealed that MSCs cultured with TS-bFGF produced a greater quantity of exosomes, particularly under three-dimensional culture conditions. These produced exosomes demonstrated substantial anti-inflammatory and wound-healing effects, as confirmed by nitric oxide (NO) assays and scratch assays. Taken together, we demonstrate that utilization of TS-bFGF for WJ-MSC-derived exosome production not only increases exosome yield but also enhances the potential for various applications in inflammation regulation and wound healing. |
| first_indexed | 2024-03-09T16:44:45Z |
| format | Article |
| id | doaj.art-2e65965e250c49fa9e6e343147fccaf5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:44:45Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2e65965e250c49fa9e6e343147fccaf52023-11-24T14:47:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221646010.3390/ijms242216460Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular VesiclesSangRok Park0SeJong Kim1KyungMin Lim2YeoKyung Shin3Kwonwoo Song4Geun-Ho Kang5Dae Young Kim6Hang-Cheol Shin7Ssang-Goo Cho8Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaPnP Biopharm Co., Ltd., 1304, Acetechnotower 8-cha, 11 Digital-ro 33-gil, Guro-gu, Seoul 08380, Republic of KoreaPnP Biopharm Co., Ltd., 1304, Acetechnotower 8-cha, 11 Digital-ro 33-gil, Guro-gu, Seoul 08380, Republic of KoreaDepartment of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaWharton’s jelly-derived mesenchymal stem cell (WJ-MSC)-derived exosomes contain a diverse cargo and exhibit remarkable biological activity, rendering them suitable for regenerative and immune-modulating functions. However, the quantity of secretion is insufficient. A large body of prior work has investigated the use of various growth factors to enhance MSC-derived exosome production. In this study, we evaluated the utilization of thermostable basic fibroblast growth factor (TS-bFGF) with MSC culture and exosome production. MSCs cultured with TS-bFGF displayed superior proliferation, as evidenced by cell cycle analysis, compared with wild-type bFGF (WT-bFGF). Stemness was assessed through mRNA expression level and colony-forming unit (CFU) assays. Furthermore, nanoparticle tracking analysis (NTA) measurements revealed that MSCs cultured with TS-bFGF produced a greater quantity of exosomes, particularly under three-dimensional culture conditions. These produced exosomes demonstrated substantial anti-inflammatory and wound-healing effects, as confirmed by nitric oxide (NO) assays and scratch assays. Taken together, we demonstrate that utilization of TS-bFGF for WJ-MSC-derived exosome production not only increases exosome yield but also enhances the potential for various applications in inflammation regulation and wound healing.https://www.mdpi.com/1422-0067/24/22/16460basic fibroblast growth factorthermostableWharton’s jelly mesenchymal stem cellexosomewound healinganti-inflammation |
| spellingShingle | SangRok Park SeJong Kim KyungMin Lim YeoKyung Shin Kwonwoo Song Geun-Ho Kang Dae Young Kim Hang-Cheol Shin Ssang-Goo Cho Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles International Journal of Molecular Sciences basic fibroblast growth factor thermostable Wharton’s jelly mesenchymal stem cell exosome wound healing anti-inflammation |
| title | Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles |
| title_full | Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles |
| title_fullStr | Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles |
| title_full_unstemmed | Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles |
| title_short | Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles |
| title_sort | thermostable basic fibroblast growth factor enhances the production and activity of human wharton s jelly mesenchymal stem cell derived extracellular vesicles |
| topic | basic fibroblast growth factor thermostable Wharton’s jelly mesenchymal stem cell exosome wound healing anti-inflammation |
| url | https://www.mdpi.com/1422-0067/24/22/16460 |
| work_keys_str_mv | AT sangrokpark thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT sejongkim thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT kyungminlim thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT yeokyungshin thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT kwonwoosong thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT geunhokang thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT daeyoungkim thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT hangcheolshin thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles AT ssanggoocho thermostablebasicfibroblastgrowthfactorenhancestheproductionandactivityofhumanwhartonsjellymesenchymalstemcellderivedextracellularvesicles |