<i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom Symbiosis
Among oral microbiota methanogens, <i>Methanobrevibacter massiliense</i> (<i>M. massiliense</i>) has remained less studied than the well-characterised and cultivated methanogens <i>Methanobrevibacter oralis</i> and <i>Methanobrevibacter smithii</i>. &l...
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MDPI AG
2024-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/1/215 |
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| author | Virginie Pilliol Mamadou Beye Laureline Terlier Julien Balmelle Idir Kacel Romain Lan Gérard Aboudharam Ghiles Grine Elodie Terrer |
| author_facet | Virginie Pilliol Mamadou Beye Laureline Terlier Julien Balmelle Idir Kacel Romain Lan Gérard Aboudharam Ghiles Grine Elodie Terrer |
| author_sort | Virginie Pilliol |
| collection | DOAJ |
| description | Among oral microbiota methanogens, <i>Methanobrevibacter massiliense</i> (<i>M. massiliense</i>) has remained less studied than the well-characterised and cultivated methanogens <i>Methanobrevibacter oralis</i> and <i>Methanobrevibacter smithii</i>. <i>M. massiliense</i> has been associated with different oral pathologies and was co-isolated with the <i>Synergistetes</i> bacterium <i>Pyramidobacter piscolens</i> (<i>P. piscolens</i>) in one case of severe periodontitis. Here, reporting on two additional necrotic pulp cases yielded the opportunity to characterise two co-cultivated <i>M. massiliense</i> isolates, both with <i>P. piscolens</i>, as non-motile, 1–2-µm-long and 0.6–0.8-µm-wide Gram-positive coccobacilli which were autofluorescent at 420 nm. The two whole genome sequences featured a 31.3% GC content, gapless 1,834,388-base-pair chromosome exhibiting an 85.9% coding ratio, encoding a formate dehydrogenase promoting <i>M. massiliense</i> growth without hydrogen in GG medium. These data pave the way to understanding a symbiotic, transkingdom association with <i>P. piscolens</i> and its role in oral pathologies. |
| first_indexed | 2024-03-08T09:49:28Z |
| format | Article |
| id | doaj.art-2e685fc814e4407986a49ce7d5c653f5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T09:49:28Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2e685fc814e4407986a49ce7d5c653f52024-01-29T14:07:05ZengMDPI AGMicroorganisms2076-26072024-01-0112121510.3390/microorganisms12010215<i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom SymbiosisVirginie Pilliol0Mamadou Beye1Laureline Terlier2Julien Balmelle3Idir Kacel4Romain Lan5Gérard Aboudharam6Ghiles Grine7Elodie Terrer8IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, 13005 Marseille, FranceIHU Méditerranée Infection, 13005 Marseille, FranceIRD, AP-HM, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, 13005 Marseille, FranceIRD, AP-HM, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, 13005 Marseille, FranceIHU Méditerranée Infection, 13005 Marseille, FranceEcole de Médecine Dentaire, Aix-Marseille University, 13385 Marseille, FranceIRD, AP-HM, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, 13005 Marseille, FranceIRD, AP-HM, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, 13005 Marseille, FranceIRD, AP-HM, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, 13005 Marseille, FranceAmong oral microbiota methanogens, <i>Methanobrevibacter massiliense</i> (<i>M. massiliense</i>) has remained less studied than the well-characterised and cultivated methanogens <i>Methanobrevibacter oralis</i> and <i>Methanobrevibacter smithii</i>. <i>M. massiliense</i> has been associated with different oral pathologies and was co-isolated with the <i>Synergistetes</i> bacterium <i>Pyramidobacter piscolens</i> (<i>P. piscolens</i>) in one case of severe periodontitis. Here, reporting on two additional necrotic pulp cases yielded the opportunity to characterise two co-cultivated <i>M. massiliense</i> isolates, both with <i>P. piscolens</i>, as non-motile, 1–2-µm-long and 0.6–0.8-µm-wide Gram-positive coccobacilli which were autofluorescent at 420 nm. The two whole genome sequences featured a 31.3% GC content, gapless 1,834,388-base-pair chromosome exhibiting an 85.9% coding ratio, encoding a formate dehydrogenase promoting <i>M. massiliense</i> growth without hydrogen in GG medium. These data pave the way to understanding a symbiotic, transkingdom association with <i>P. piscolens</i> and its role in oral pathologies.https://www.mdpi.com/2076-2607/12/1/215<i>Archaea</i>methanogen<i>Methanobrevibacter massiliense</i><i>Synergistetes</i><i>Pyramidobacter piscolens</i>hydrogen-free culture |
| spellingShingle | Virginie Pilliol Mamadou Beye Laureline Terlier Julien Balmelle Idir Kacel Romain Lan Gérard Aboudharam Ghiles Grine Elodie Terrer <i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom Symbiosis Microorganisms <i>Archaea</i> methanogen <i>Methanobrevibacter massiliense</i> <i>Synergistetes</i> <i>Pyramidobacter piscolens</i> hydrogen-free culture |
| title | <i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom Symbiosis |
| title_full | <i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom Symbiosis |
| title_fullStr | <i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom Symbiosis |
| title_full_unstemmed | <i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom Symbiosis |
| title_short | <i>Methanobrevibacter massiliense</i> and <i>Pyramidobacter piscolens</i> Co-Culture Illustrates Transkingdom Symbiosis |
| title_sort | i methanobrevibacter massiliense i and i pyramidobacter piscolens i co culture illustrates transkingdom symbiosis |
| topic | <i>Archaea</i> methanogen <i>Methanobrevibacter massiliense</i> <i>Synergistetes</i> <i>Pyramidobacter piscolens</i> hydrogen-free culture |
| url | https://www.mdpi.com/2076-2607/12/1/215 |
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