Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study
The T2Bacteria panel is a direct-from-blood assay that delivers rapid results, targeting <i>E. coli</i>, <i>S. aureus</i>, <i>K. pneumoniae</i>, <i>A. baumanii</i>, <i>P. aeruginosa</i>, and <i>E. faecium</i> (ESKAPE pathogens)....
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/8/832 |
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| author | Anders Krifors Måns Ullberg Markus Castegren Johan Petersson Ernesto Sparrelid Volkan Özenci Ola Blennow |
| author_facet | Anders Krifors Måns Ullberg Markus Castegren Johan Petersson Ernesto Sparrelid Volkan Özenci Ola Blennow |
| author_sort | Anders Krifors |
| collection | DOAJ |
| description | The T2Bacteria panel is a direct-from-blood assay that delivers rapid results, targeting <i>E. coli</i>, <i>S. aureus</i>, <i>K. pneumoniae</i>, <i>A. baumanii</i>, <i>P. aeruginosa</i>, and <i>E. faecium</i> (ESKAPE pathogens). In this study, T2Bacteria and T2Candida (targeting <i>C. albicans</i>/<i>C. tropicalis</i>, <i>C. glabrata</i>/<i>C. krusei</i>, and <i>C. parapsilosis</i>) were evaluated in parallel with blood cultures in 101 consecutive surgical patients with suspected intra-abdominal infection admitted to the intensive care unit or high dependency unit. Fifteen patients had bacteremia, with T2Bacteria correctly identifying all on-panel (<i>n</i> = 8) pathogens. T2Bacteria was positive in 19 additional patients, 11 of whom had supportive cultures from other normally sterile sites (newly inserted drains, perioperative cultures or blood cultures) within seven days. Six of these eleven patients (55%) received broad-spectrum antibiotics at the sampling time. T2Candida identified the two cases of blood-culture-positive candidemia and was positive in seven additional patients, three of whom were confirmed to have intra-abdominal candidiasis. Of four patients with concurrent T2Bacteria and T2Candida positivity, only one patient had positive blood cultures (candidemia), while three out of four patients had supporting microbiological evidence of a mixed infection. T2Bacteria and T2Candida were fast and accurate in diagnosing on-panel bloodstream infections, and T2Bacteria was able to detect culture-negative intra-abdominal infections. |
| first_indexed | 2024-03-09T09:53:38Z |
| format | Article |
| id | doaj.art-2e6ad52b08be47d296bee3a5802b1b82 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T09:53:38Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-2e6ad52b08be47d296bee3a5802b1b822023-12-01T23:52:17ZengMDPI AGJournal of Fungi2309-608X2022-08-018883210.3390/jof8080832Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter StudyAnders Krifors0Måns Ullberg1Markus Castegren2Johan Petersson3Ernesto Sparrelid4Volkan Özenci5Ola Blennow6Department of Physiology and Pharmacology, Karolinska Institutet, 14186 Stockholm, SwedenDepartment of Clinical Microbiology, Karolinska University Hospital, 14186 Stockholm, SwedenDepartment of Physiology and Pharmacology, Karolinska Institutet, 14186 Stockholm, SwedenDepartment of Physiology and Pharmacology, Karolinska Institutet, 14186 Stockholm, SwedenDepartment of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 14186 Stockholm, SwedenDepartment of Clinical Microbiology, Karolinska University Hospital, 14186 Stockholm, SwedenDepartment of Infectious Diseases, Karolinska University Hospital, 14186 Stockholm, SwedenThe T2Bacteria panel is a direct-from-blood assay that delivers rapid results, targeting <i>E. coli</i>, <i>S. aureus</i>, <i>K. pneumoniae</i>, <i>A. baumanii</i>, <i>P. aeruginosa</i>, and <i>E. faecium</i> (ESKAPE pathogens). In this study, T2Bacteria and T2Candida (targeting <i>C. albicans</i>/<i>C. tropicalis</i>, <i>C. glabrata</i>/<i>C. krusei</i>, and <i>C. parapsilosis</i>) were evaluated in parallel with blood cultures in 101 consecutive surgical patients with suspected intra-abdominal infection admitted to the intensive care unit or high dependency unit. Fifteen patients had bacteremia, with T2Bacteria correctly identifying all on-panel (<i>n</i> = 8) pathogens. T2Bacteria was positive in 19 additional patients, 11 of whom had supportive cultures from other normally sterile sites (newly inserted drains, perioperative cultures or blood cultures) within seven days. Six of these eleven patients (55%) received broad-spectrum antibiotics at the sampling time. T2Candida identified the two cases of blood-culture-positive candidemia and was positive in seven additional patients, three of whom were confirmed to have intra-abdominal candidiasis. Of four patients with concurrent T2Bacteria and T2Candida positivity, only one patient had positive blood cultures (candidemia), while three out of four patients had supporting microbiological evidence of a mixed infection. T2Bacteria and T2Candida were fast and accurate in diagnosing on-panel bloodstream infections, and T2Bacteria was able to detect culture-negative intra-abdominal infections.https://www.mdpi.com/2309-608X/8/8/832T2 magnetic resonanceT2Bacteriaintra-abdominal infection |
| spellingShingle | Anders Krifors Måns Ullberg Markus Castegren Johan Petersson Ernesto Sparrelid Volkan Özenci Ola Blennow Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study Journal of Fungi T2 magnetic resonance T2Bacteria intra-abdominal infection |
| title | Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study |
| title_full | Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study |
| title_fullStr | Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study |
| title_full_unstemmed | Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study |
| title_short | Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study |
| title_sort | combining t2bacteria and t2candida panels for diagnosing intra abdominal infections a prospective multicenter study |
| topic | T2 magnetic resonance T2Bacteria intra-abdominal infection |
| url | https://www.mdpi.com/2309-608X/8/8/832 |
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