Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat
Background/Aims: We aimed to evaluate whether pancreatic kininogenase (PKase) can relieve renal fibrosis and investigate its mechanisms in diabetic nephropathy (DN) rats Methods: We established streptozotocin (STZ) induced-DN rats. After treatment with PKase for 4 weeks, urinary weight, urinary prot...
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| Format: | Article |
| Language: | English |
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Karger Publishers
2016-01-01
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| Series: | Kidney & Blood Pressure Research |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/368542 |
| _version_ | 1818541560412766208 |
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| author | Dan Zhu Linlin Zhang Lijuan Cheng Liansheng Ren Jie Tang Dianjun Sun |
| author_facet | Dan Zhu Linlin Zhang Lijuan Cheng Liansheng Ren Jie Tang Dianjun Sun |
| author_sort | Dan Zhu |
| collection | DOAJ |
| description | Background/Aims: We aimed to evaluate whether pancreatic kininogenase (PKase) can relieve renal fibrosis and investigate its mechanisms in diabetic nephropathy (DN) rats Methods: We established streptozotocin (STZ) induced-DN rats. After treatment with PKase for 4 weeks, urinary weight, urinary protein content and blood glucose concentration were detected, and then renal histopathological changes were examined using Hematoxylin and Eosin (H&E) and Masson's thrchrome staining. In addition, the expressions of miR-433, transforming growth factor-β1 (TGF-β1) and antizyme inhibitor 1 (Azin1) were detected by qRT-PCR and/or western blotting. Results: PKase reduced urinary weight, urinary protein contents and blood glucose concentrations. PKase treated DN rats exhibited less renal fibrosis than untreated DN rats (P P P Conclusions: PKase might not only inhibit the development of DN by reducing urinary weight, urinary protein content and blood glucose concentration in DN rats, but also relieve renal fibrosis in DN rats through inhibiting the expression of TGF-β1, and miR-433 and Azin1 might involve in this process. |
| first_indexed | 2024-12-11T22:10:53Z |
| format | Article |
| id | doaj.art-2e6b58d36ad64c0d90dbb2ac39a22574 |
| institution | Directory Open Access Journal |
| issn | 1420-4096 1423-0143 |
| language | English |
| last_indexed | 2024-12-11T22:10:53Z |
| publishDate | 2016-01-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Kidney & Blood Pressure Research |
| spelling | doaj.art-2e6b58d36ad64c0d90dbb2ac39a225742022-12-22T00:48:46ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432016-01-0141191710.1159/000368542368542Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy RatDan ZhuLinlin ZhangLijuan ChengLiansheng RenJie TangDianjun SunBackground/Aims: We aimed to evaluate whether pancreatic kininogenase (PKase) can relieve renal fibrosis and investigate its mechanisms in diabetic nephropathy (DN) rats Methods: We established streptozotocin (STZ) induced-DN rats. After treatment with PKase for 4 weeks, urinary weight, urinary protein content and blood glucose concentration were detected, and then renal histopathological changes were examined using Hematoxylin and Eosin (H&E) and Masson's thrchrome staining. In addition, the expressions of miR-433, transforming growth factor-β1 (TGF-β1) and antizyme inhibitor 1 (Azin1) were detected by qRT-PCR and/or western blotting. Results: PKase reduced urinary weight, urinary protein contents and blood glucose concentrations. PKase treated DN rats exhibited less renal fibrosis than untreated DN rats (P P P Conclusions: PKase might not only inhibit the development of DN by reducing urinary weight, urinary protein content and blood glucose concentration in DN rats, but also relieve renal fibrosis in DN rats through inhibiting the expression of TGF-β1, and miR-433 and Azin1 might involve in this process.http://www.karger.com/Article/FullText/368542Transforming growth factor-β1Pancreatic KininogenaseRenal fibrosisAntizyme inhibitor 1MiR-433 |
| spellingShingle | Dan Zhu Linlin Zhang Lijuan Cheng Liansheng Ren Jie Tang Dianjun Sun Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat Kidney & Blood Pressure Research Transforming growth factor-β1 Pancreatic Kininogenase Renal fibrosis Antizyme inhibitor 1 MiR-433 |
| title | Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat |
| title_full | Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat |
| title_fullStr | Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat |
| title_full_unstemmed | Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat |
| title_short | Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat |
| title_sort | pancreatic kininogenase ameliorates renal fibrosis in streptozotocin induced diabetic nephropathy rat |
| topic | Transforming growth factor-β1 Pancreatic Kininogenase Renal fibrosis Antizyme inhibitor 1 MiR-433 |
| url | http://www.karger.com/Article/FullText/368542 |
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