Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i>
Fucoxanthin is an oxygenated carotenoid component that has been reported to play important roles in anti-oxidation, anti-obesity and anti-cancer in the human body. Fucoxanthin-chlorophyll protein (FCP) complexes participate in light harvesting and photoprotection in diatom. In order to better unders...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/10/644 |
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| author | Shuaiqi Zhu Song Bin Wenda Wang Shan Lu Wenqiang Yang |
| author_facet | Shuaiqi Zhu Song Bin Wenda Wang Shan Lu Wenqiang Yang |
| author_sort | Shuaiqi Zhu |
| collection | DOAJ |
| description | Fucoxanthin is an oxygenated carotenoid component that has been reported to play important roles in anti-oxidation, anti-obesity and anti-cancer in the human body. Fucoxanthin-chlorophyll protein (FCP) complexes participate in light harvesting and photoprotection in diatom. In order to better understand the change of fucoxanthin content and its role in photoprotection, the growth, fucoxanthin biosynthesis and photosynthetic phenotypes were studied in the diatom <i>Phaeodactylum tricornutum</i> under the treatment of exogenous arachidonic acid (AA). Our results demonstrated that even low concentration of AA at 0.1 mg/L strongly induced fucoxanthin accumulation in algal cells to a maximum of 1.1 mg/g dry weight (DW), which was 36.6% higher than that in the untreated ones. By principal component analysis (PCA), we also identified a close correlation between fucoxanthin accumulation and the expression of genes involved in fucoxanthin biosynthesis, especially phytoene synthase (PSY), suggesting that AA change the metabolism of fucoxanthin by inducing carotenoid metabolic enzymes at the transcriptional level. Furthermore, we found that the exogenous application of AA affected non-photochemical quenching (NPQ) and photoinhibition, which resulted from the changed diadinoxanthin (DD) and diatoxanthin (DT) cycle, and thus played an important role in photoprotection. |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T03:33:17Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-2e6c8e2131ab437cb5c54fd32dfe85a12023-12-03T14:51:16ZengMDPI AGMarine Drugs1660-33972022-10-01201064410.3390/md20100644Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i>Shuaiqi Zhu0Song Bin1Wenda Wang2Shan Lu3Wenqiang Yang4State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaFucoxanthin is an oxygenated carotenoid component that has been reported to play important roles in anti-oxidation, anti-obesity and anti-cancer in the human body. Fucoxanthin-chlorophyll protein (FCP) complexes participate in light harvesting and photoprotection in diatom. In order to better understand the change of fucoxanthin content and its role in photoprotection, the growth, fucoxanthin biosynthesis and photosynthetic phenotypes were studied in the diatom <i>Phaeodactylum tricornutum</i> under the treatment of exogenous arachidonic acid (AA). Our results demonstrated that even low concentration of AA at 0.1 mg/L strongly induced fucoxanthin accumulation in algal cells to a maximum of 1.1 mg/g dry weight (DW), which was 36.6% higher than that in the untreated ones. By principal component analysis (PCA), we also identified a close correlation between fucoxanthin accumulation and the expression of genes involved in fucoxanthin biosynthesis, especially phytoene synthase (PSY), suggesting that AA change the metabolism of fucoxanthin by inducing carotenoid metabolic enzymes at the transcriptional level. Furthermore, we found that the exogenous application of AA affected non-photochemical quenching (NPQ) and photoinhibition, which resulted from the changed diadinoxanthin (DD) and diatoxanthin (DT) cycle, and thus played an important role in photoprotection.https://www.mdpi.com/1660-3397/20/10/644gene expressioncarotenoidprincipal component analysislight harvestingnon-photochemical quenching |
| spellingShingle | Shuaiqi Zhu Song Bin Wenda Wang Shan Lu Wenqiang Yang Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i> Marine Drugs gene expression carotenoid principal component analysis light harvesting non-photochemical quenching |
| title | Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i> |
| title_full | Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i> |
| title_fullStr | Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i> |
| title_full_unstemmed | Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i> |
| title_short | Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in <i>Phaeodactylum tricornutum</i> |
| title_sort | exogenous arachidonic acid affects fucoxanthin biosynthesis and photoprotection in i phaeodactylum tricornutum i |
| topic | gene expression carotenoid principal component analysis light harvesting non-photochemical quenching |
| url | https://www.mdpi.com/1660-3397/20/10/644 |
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