A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S]
The polar lipids of the anaerobic bacterium Clostridium tetani, the causative agent of tetanus, have been examined by two-dimensional thin layer chromatography, ESI mass spectrometry, and NMR spectroscopy. Plasmalogen and di- and tetra-acylated species of phosphatidylethanolamine, phosphatidylglycer...
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| Format: | Article |
| Language: | English |
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Elsevier
2010-07-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520371170 |
| _version_ | 1819077224624553984 |
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| author | Norah C. Johnston Semra Aygun-Sunar Ziqiang Guan Anthony A. Ribeiro Fevzi Daldal Christian R.H. Raetz Howard Goldfine |
| author_facet | Norah C. Johnston Semra Aygun-Sunar Ziqiang Guan Anthony A. Ribeiro Fevzi Daldal Christian R.H. Raetz Howard Goldfine |
| author_sort | Norah C. Johnston |
| collection | DOAJ |
| description | The polar lipids of the anaerobic bacterium Clostridium tetani, the causative agent of tetanus, have been examined by two-dimensional thin layer chromatography, ESI mass spectrometry, and NMR spectroscopy. Plasmalogen and di- and tetra-acylated species of phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and N-acetylglucosaminyl diradylglycerol were the major lipids present in most strains examined except for strain ATCC 10779, the parent of strain E88, the first C. tetani strain to have its genome sequenced. This strain contained the same di- and tetra-acylated species but did not contain plasmalogens. All strains contained a novel derivative of N-acetylglucosaminyl diradylglycerol in which a phosphoethanolamine unit is attached to the 6’-position of the sugar, as judged by selective 31P-decoupled, 1H-detected NMR difference spectroscopy. The N-acetylglucosamine (GlcNAc) residue is presumably linked to the 3-positon of the diradylglycerol moiety, and it has the β-anomeric configuration. Very little plasmalogen component was detected by mass spectrometry in the precursors phosphatidic acid and phosphatidylserine, consistent with the idea that plasmalogens are formed from diacylated phospholipids at a late stage of phospholipid assembly in anaerobic clostridia. |
| first_indexed | 2024-12-21T18:53:48Z |
| format | Article |
| id | doaj.art-2e6ce662a30a478492689de6adf7d4ac |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:53:48Z |
| publishDate | 2010-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-2e6ce662a30a478492689de6adf7d4ac2022-12-21T18:53:41ZengElsevierJournal of Lipid Research0022-22752010-07-0151719531961A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S]Norah C. Johnston0Semra Aygun-Sunar1Ziqiang Guan2Anthony A. Ribeiro3Fevzi Daldal4Christian R.H. Raetz5Howard Goldfine6Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PADepartment of Biology, University of Pennsylvania, Philadelphia, PADepartment of Biochemistry, Duke University Medical Center, Durham, NCDepartment of Biochemistry, Duke University Medical Center, Durham, NCDepartment of Biology, University of Pennsylvania, Philadelphia, PADepartment of Biochemistry, Duke University Medical Center, Durham, NCTo whom correspondence should be addressed:; Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PAThe polar lipids of the anaerobic bacterium Clostridium tetani, the causative agent of tetanus, have been examined by two-dimensional thin layer chromatography, ESI mass spectrometry, and NMR spectroscopy. Plasmalogen and di- and tetra-acylated species of phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and N-acetylglucosaminyl diradylglycerol were the major lipids present in most strains examined except for strain ATCC 10779, the parent of strain E88, the first C. tetani strain to have its genome sequenced. This strain contained the same di- and tetra-acylated species but did not contain plasmalogens. All strains contained a novel derivative of N-acetylglucosaminyl diradylglycerol in which a phosphoethanolamine unit is attached to the 6’-position of the sugar, as judged by selective 31P-decoupled, 1H-detected NMR difference spectroscopy. The N-acetylglucosamine (GlcNAc) residue is presumably linked to the 3-positon of the diradylglycerol moiety, and it has the β-anomeric configuration. Very little plasmalogen component was detected by mass spectrometry in the precursors phosphatidic acid and phosphatidylserine, consistent with the idea that plasmalogens are formed from diacylated phospholipids at a late stage of phospholipid assembly in anaerobic clostridia.http://www.sciencedirect.com/science/article/pii/S0022227520371170glycolipidmass spectrometrynuclear magnetic resonance spectroscopyphospholipidplasmalogen |
| spellingShingle | Norah C. Johnston Semra Aygun-Sunar Ziqiang Guan Anthony A. Ribeiro Fevzi Daldal Christian R.H. Raetz Howard Goldfine A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S] Journal of Lipid Research glycolipid mass spectrometry nuclear magnetic resonance spectroscopy phospholipid plasmalogen |
| title | A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S] |
| title_full | A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S] |
| title_fullStr | A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S] |
| title_full_unstemmed | A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S] |
| title_short | A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani[S] |
| title_sort | phosphoethanolamine modified glycosyl diradylglycerol in the polar lipids of clostridium tetani s |
| topic | glycolipid mass spectrometry nuclear magnetic resonance spectroscopy phospholipid plasmalogen |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520371170 |
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