Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair
Cardiac repair following MI relies on a finely regulated immune response involving sequential recruitment of monocytes to the injured tissue. Monocyte-derived cells are also critical for tissue homeostasis and healing process. Our previous findings demonstrated the interaction of T and natural kille...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01413/full |
| _version_ | 1811214764478562304 |
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| author | Noémie Dam Noémie Dam Hocine Rachid Hocine Itziar Palacios Olga DelaRosa Ramón Menta Dominique Charron Dominique Charron Armand Bensussan Hicham El Costa Nabila Jabrane-Ferrat Wilfried Dalemans Eleuterio Lombardo Reem Al-Daccak Reem Al-Daccak |
| author_facet | Noémie Dam Noémie Dam Hocine Rachid Hocine Itziar Palacios Olga DelaRosa Ramón Menta Dominique Charron Dominique Charron Armand Bensussan Hicham El Costa Nabila Jabrane-Ferrat Wilfried Dalemans Eleuterio Lombardo Reem Al-Daccak Reem Al-Daccak |
| author_sort | Noémie Dam |
| collection | DOAJ |
| description | Cardiac repair following MI relies on a finely regulated immune response involving sequential recruitment of monocytes to the injured tissue. Monocyte-derived cells are also critical for tissue homeostasis and healing process. Our previous findings demonstrated the interaction of T and natural killer cells with allogeneic human cardiac-derived stem/progenitor cells (hCPC) and suggested their beneficial effect in the context of cardiac repair. Therefore, we investigated here whether monocytes and their descendants could be also modulated by allogeneic hCPC toward a repair/anti-inflammatory phenotype. Through experimental in vitro assays, we assessed the impact of allogeneic hCPC on the recruitment, functions and differentiation of monocytes. We found that allogeneic hCPC at steady state or under inflammatory conditions can incite CCL-2/CCR2-dependent recruitment of circulating CD14+CD16− monocytes and fine-tune their activation toward an anti-inflammatory profile. Allogeneic hCPC also promoted CD14+CD16− monocyte polarization into anti-inflammatory/immune-regulatory macrophages with high phagocytic capacity and IL10 secretion. Moreover, hCPC bended the differentiation of CD14+CD16− monocytes to dendritic cells (DCs) toward anti-inflammatory macrophage-like features and impaired their antigen-presenting function in favor of immune-modulation. Collectively, our results demonstrate that allogeneic hCPC could reshape monocytes, macrophages as well as DCs responses by favoring their anti-inflammatory/tolerogenic activation/polarization. Thereby, therapeutic allogeneic hCPC might also contribute to post-infarct myocardial healing by modeling the activities of monocytes and their derived descendants. |
| first_indexed | 2024-04-12T06:10:23Z |
| format | Article |
| id | doaj.art-2e750335f2af4407ba2e035fd50ab6ec |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T06:10:23Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-2e750335f2af4407ba2e035fd50ab6ec2022-12-22T03:44:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-10-01810.3389/fimmu.2017.01413300162Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac RepairNoémie Dam0Noémie Dam1Hocine Rachid Hocine2Itziar Palacios3Olga DelaRosa4Ramón Menta5Dominique Charron6Dominique Charron7Armand Bensussan8Hicham El Costa9Nabila Jabrane-Ferrat10Wilfried Dalemans11Eleuterio Lombardo12Reem Al-Daccak13Reem Al-Daccak14Coretherapix SLU, Tigenix Group, Madrid, SpainInstitut National de la Santé et de la Recherche Médicale (INSERM) UMRS-976, Université Paris-Diderot, Hôpital Saint-Louis, Paris, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM) UMRS-976, Université Paris-Diderot, Hôpital Saint-Louis, Paris, FranceCoretherapix SLU, Tigenix Group, Madrid, SpainTigenix, Madrid, SpainCoretherapix SLU, Tigenix Group, Madrid, SpainInstitut National de la Santé et de la Recherche Médicale (INSERM) UMRS-976, Université Paris-Diderot, Hôpital Saint-Louis, Paris, FranceHLA et Médecine, Hôpital Saint Louis, Paris, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM) UMRS-976, Université Paris-Diderot, Hôpital Saint-Louis, Paris, FranceCentre National de la Recherche Scientifique (CNRS), Centre of Pathophysiology Toulouse Purpan, INSERM, Université Toulouse III, CHU Purpan, Toulouse, FranceCentre National de la Recherche Scientifique (CNRS), Centre of Pathophysiology Toulouse Purpan, INSERM, Université Toulouse III, CHU Purpan, Toulouse, FranceTigenix NV, Leuven, BelgiumTigenix, Madrid, SpainInstitut National de la Santé et de la Recherche Médicale (INSERM) UMRS-976, Université Paris-Diderot, Hôpital Saint-Louis, Paris, FranceHLA et Médecine, Hôpital Saint Louis, Paris, FranceCardiac repair following MI relies on a finely regulated immune response involving sequential recruitment of monocytes to the injured tissue. Monocyte-derived cells are also critical for tissue homeostasis and healing process. Our previous findings demonstrated the interaction of T and natural killer cells with allogeneic human cardiac-derived stem/progenitor cells (hCPC) and suggested their beneficial effect in the context of cardiac repair. Therefore, we investigated here whether monocytes and their descendants could be also modulated by allogeneic hCPC toward a repair/anti-inflammatory phenotype. Through experimental in vitro assays, we assessed the impact of allogeneic hCPC on the recruitment, functions and differentiation of monocytes. We found that allogeneic hCPC at steady state or under inflammatory conditions can incite CCL-2/CCR2-dependent recruitment of circulating CD14+CD16− monocytes and fine-tune their activation toward an anti-inflammatory profile. Allogeneic hCPC also promoted CD14+CD16− monocyte polarization into anti-inflammatory/immune-regulatory macrophages with high phagocytic capacity and IL10 secretion. Moreover, hCPC bended the differentiation of CD14+CD16− monocytes to dendritic cells (DCs) toward anti-inflammatory macrophage-like features and impaired their antigen-presenting function in favor of immune-modulation. Collectively, our results demonstrate that allogeneic hCPC could reshape monocytes, macrophages as well as DCs responses by favoring their anti-inflammatory/tolerogenic activation/polarization. Thereby, therapeutic allogeneic hCPC might also contribute to post-infarct myocardial healing by modeling the activities of monocytes and their derived descendants.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01413/fullmonocytesmacrophagesdendritic cellscardiac-derived stem/progenitor cellsallogeneic stem cells therapy |
| spellingShingle | Noémie Dam Noémie Dam Hocine Rachid Hocine Itziar Palacios Olga DelaRosa Ramón Menta Dominique Charron Dominique Charron Armand Bensussan Hicham El Costa Nabila Jabrane-Ferrat Wilfried Dalemans Eleuterio Lombardo Reem Al-Daccak Reem Al-Daccak Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair Frontiers in Immunology monocytes macrophages dendritic cells cardiac-derived stem/progenitor cells allogeneic stem cells therapy |
| title | Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair |
| title_full | Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair |
| title_fullStr | Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair |
| title_full_unstemmed | Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair |
| title_short | Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair |
| title_sort | human cardiac derived stem progenitor cells fine tune monocyte derived descendants activities toward cardiac repair |
| topic | monocytes macrophages dendritic cells cardiac-derived stem/progenitor cells allogeneic stem cells therapy |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01413/full |
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