An ameloblastin C-terminus variant is present in human adipose tissue
Objective: Transcriptional regulatory elements in the ameloblastin (AMBN) promoter indicate that adipogenesis may influence its expression. The objective here was to investigate if AMBN is expressed in adipose tissue, and have a role during differentiation of adipocytes. Design: AMBN expression was...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844018316438 |
| _version_ | 1819082754034237440 |
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| author | Øystein Stakkestad Catherine Heyward Ståle Petter Lyngstadaas Tirill Medin Jiri Vondrasek Aina-Mari Lian Gita Pezeshki Janne Elin Reseland |
| author_facet | Øystein Stakkestad Catherine Heyward Ståle Petter Lyngstadaas Tirill Medin Jiri Vondrasek Aina-Mari Lian Gita Pezeshki Janne Elin Reseland |
| author_sort | Øystein Stakkestad |
| collection | DOAJ |
| description | Objective: Transcriptional regulatory elements in the ameloblastin (AMBN) promoter indicate that adipogenesis may influence its expression. The objective here was to investigate if AMBN is expressed in adipose tissue, and have a role during differentiation of adipocytes. Design: AMBN expression was examined in adipose tissue and adipocytes by real-time PCR and ELISA. Distribution of ameloblastin was investigated by immunofluorescence in sections of human subcutaneous adipose tissue. The effect of recombinant proteins resembling AMBN and its processed products on proliferation of primary human pre-adipocytes and murine 3T3-L1 cell lines was measured by [3H]-thymidine incorporation. The effect on adipocyte differentiation was evaluated by the expression profile of the adipogenic markers PPARγ and leptin, and the content of lipids droplets (Oil-Red-O staining). Results: AMBN was found to be expressed in human adipose tissue, human primary adipocytes, and in 3T3-L1 cells. The C-terminus of the AMBN protein and a 45 bp shorter splice variant was identified in human subcutaneous adipose tissue. The expression of AMBN was found to increase four-fold during differentiation of 3T3-L1 cells. Administration of recombinant AMBN reduced the proliferation, and enhanced the expression of PPARγ and leptin in 3T3-L1 and human pre-adipocytes, respectively. Conclusions: The AMBN C-terminus variant was identified in adipocytes. This variant may be encoded from a short splice variant. Increased expression of AMBN during adipogenesis and its effect on adipogenic factors suggests that AMBN also has a role in adipocyte development. |
| first_indexed | 2024-12-21T20:21:41Z |
| format | Article |
| id | doaj.art-2e7763968c1a433da29788f920efb9a7 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-21T20:21:41Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-2e7763968c1a433da29788f920efb9a72022-12-21T18:51:28ZengElsevierHeliyon2405-84402018-12-01412e01075An ameloblastin C-terminus variant is present in human adipose tissueØystein Stakkestad0Catherine Heyward1Ståle Petter Lyngstadaas2Tirill Medin3Jiri Vondrasek4Aina-Mari Lian5Gita Pezeshki6Janne Elin Reseland7Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, NorwayDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, NorwayDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, NorwayDepartment of Nursing and Health Promotion, Faculty of Health Sciences, OsloMet – Oslo Metropolitan University, NorwayDepartment of Bioinformatics, Institute of Organic Chemistry and Biology, Czech Academy of Sciences, Prague, Czech RepublicDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, NorwayDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, NorwayDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Norway; Corresponding author.Objective: Transcriptional regulatory elements in the ameloblastin (AMBN) promoter indicate that adipogenesis may influence its expression. The objective here was to investigate if AMBN is expressed in adipose tissue, and have a role during differentiation of adipocytes. Design: AMBN expression was examined in adipose tissue and adipocytes by real-time PCR and ELISA. Distribution of ameloblastin was investigated by immunofluorescence in sections of human subcutaneous adipose tissue. The effect of recombinant proteins resembling AMBN and its processed products on proliferation of primary human pre-adipocytes and murine 3T3-L1 cell lines was measured by [3H]-thymidine incorporation. The effect on adipocyte differentiation was evaluated by the expression profile of the adipogenic markers PPARγ and leptin, and the content of lipids droplets (Oil-Red-O staining). Results: AMBN was found to be expressed in human adipose tissue, human primary adipocytes, and in 3T3-L1 cells. The C-terminus of the AMBN protein and a 45 bp shorter splice variant was identified in human subcutaneous adipose tissue. The expression of AMBN was found to increase four-fold during differentiation of 3T3-L1 cells. Administration of recombinant AMBN reduced the proliferation, and enhanced the expression of PPARγ and leptin in 3T3-L1 and human pre-adipocytes, respectively. Conclusions: The AMBN C-terminus variant was identified in adipocytes. This variant may be encoded from a short splice variant. Increased expression of AMBN during adipogenesis and its effect on adipogenic factors suggests that AMBN also has a role in adipocyte development.http://www.sciencedirect.com/science/article/pii/S2405844018316438BiochemistryCell biologyMolecular biologyPhysiology |
| spellingShingle | Øystein Stakkestad Catherine Heyward Ståle Petter Lyngstadaas Tirill Medin Jiri Vondrasek Aina-Mari Lian Gita Pezeshki Janne Elin Reseland An ameloblastin C-terminus variant is present in human adipose tissue Heliyon Biochemistry Cell biology Molecular biology Physiology |
| title | An ameloblastin C-terminus variant is present in human adipose tissue |
| title_full | An ameloblastin C-terminus variant is present in human adipose tissue |
| title_fullStr | An ameloblastin C-terminus variant is present in human adipose tissue |
| title_full_unstemmed | An ameloblastin C-terminus variant is present in human adipose tissue |
| title_short | An ameloblastin C-terminus variant is present in human adipose tissue |
| title_sort | ameloblastin c terminus variant is present in human adipose tissue |
| topic | Biochemistry Cell biology Molecular biology Physiology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844018316438 |
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