Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain Injury
Neonatal hypoxic-ischemic encephalopathy causes neurodegeneration and brain injury, leading to sensorimotor dysfunction. Xyloketal B is a novel marine compound isolated from a mangrove fungus Xylaria species (no. 2508) with unique antioxidant effects. In this study, we investigated the effects and m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-12-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/1/29 |
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| author | Ai-Jiao Xiao Wenliang Chen Baofeng Xu Rui Liu Ekaterina Turlova Andrew Barszczyk Christopher Lf Sun Ling Liu Marielle Deurloo Guan-Lei Wang Zhong-Ping Feng Hong-Shuo Sun |
| author_facet | Ai-Jiao Xiao Wenliang Chen Baofeng Xu Rui Liu Ekaterina Turlova Andrew Barszczyk Christopher Lf Sun Ling Liu Marielle Deurloo Guan-Lei Wang Zhong-Ping Feng Hong-Shuo Sun |
| author_sort | Ai-Jiao Xiao |
| collection | DOAJ |
| description | Neonatal hypoxic-ischemic encephalopathy causes neurodegeneration and brain injury, leading to sensorimotor dysfunction. Xyloketal B is a novel marine compound isolated from a mangrove fungus Xylaria species (no. 2508) with unique antioxidant effects. In this study, we investigated the effects and mechanism of xyloketal B on oxygen-glucose deprivation-induced neuronal cell death in mouse primary cortical culture and on hypoxic-ischemic brain injury in neonatal mice in vivo. We found that xyloketal B reduced anoxia-induced neuronal cell death in vitro, as well as infarct volume in neonatal hypoxic-ischemic brain injury model in vivo. Furthermore, xyloketal B improved functional behavioral recovery of the animals following hypoxic-ischemic insult. In addition, xyloketal B significantly decreased calcium entry, reduced the number of TUNEL-positive cells, reduced the levels of cleaved caspase-3 and Bax proteins, and increased the level of Bcl-2 protein after the hypoxic-ischemic injury. Our findings indicate that xyloketal B is effective in models of hypoxia-ischemia and thus has potential as a treatment for hypoxic-ischemic brain injury. |
| first_indexed | 2024-04-11T13:39:34Z |
| format | Article |
| id | doaj.art-2e77ffb40639493498684eb7479943fa |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:39:34Z |
| publishDate | 2014-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-2e77ffb40639493498684eb7479943fa2022-12-22T04:21:19ZengMDPI AGMarine Drugs1660-33972014-12-01131294710.3390/md13010029md13010029Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain InjuryAi-Jiao Xiao0Wenliang Chen1Baofeng Xu2Rui Liu3Ekaterina Turlova4Andrew Barszczyk5Christopher Lf Sun6Ling Liu7Marielle Deurloo8Guan-Lei Wang9Zhong-Ping Feng10Hong-Shuo Sun11Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, CanadaNeonatal hypoxic-ischemic encephalopathy causes neurodegeneration and brain injury, leading to sensorimotor dysfunction. Xyloketal B is a novel marine compound isolated from a mangrove fungus Xylaria species (no. 2508) with unique antioxidant effects. In this study, we investigated the effects and mechanism of xyloketal B on oxygen-glucose deprivation-induced neuronal cell death in mouse primary cortical culture and on hypoxic-ischemic brain injury in neonatal mice in vivo. We found that xyloketal B reduced anoxia-induced neuronal cell death in vitro, as well as infarct volume in neonatal hypoxic-ischemic brain injury model in vivo. Furthermore, xyloketal B improved functional behavioral recovery of the animals following hypoxic-ischemic insult. In addition, xyloketal B significantly decreased calcium entry, reduced the number of TUNEL-positive cells, reduced the levels of cleaved caspase-3 and Bax proteins, and increased the level of Bcl-2 protein after the hypoxic-ischemic injury. Our findings indicate that xyloketal B is effective in models of hypoxia-ischemia and thus has potential as a treatment for hypoxic-ischemic brain injury.http://www.mdpi.com/1660-3397/13/1/29hypoxic-ischemic injuryinfarct volumeneuroprotectionoxygen glucose deprivationprimary neuronal cell cultureneonatal strokebehavioral testsmarine drug |
| spellingShingle | Ai-Jiao Xiao Wenliang Chen Baofeng Xu Rui Liu Ekaterina Turlova Andrew Barszczyk Christopher Lf Sun Ling Liu Marielle Deurloo Guan-Lei Wang Zhong-Ping Feng Hong-Shuo Sun Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain Injury Marine Drugs hypoxic-ischemic injury infarct volume neuroprotection oxygen glucose deprivation primary neuronal cell culture neonatal stroke behavioral tests marine drug |
| title | Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain Injury |
| title_full | Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain Injury |
| title_fullStr | Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain Injury |
| title_full_unstemmed | Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain Injury |
| title_short | Marine Compound Xyloketal B Reduces Neonatal Hypoxic-Ischemic Brain Injury |
| title_sort | marine compound xyloketal b reduces neonatal hypoxic ischemic brain injury |
| topic | hypoxic-ischemic injury infarct volume neuroprotection oxygen glucose deprivation primary neuronal cell culture neonatal stroke behavioral tests marine drug |
| url | http://www.mdpi.com/1660-3397/13/1/29 |
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