Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice
The modulation of the gut microbiome has been widely suggested as a promising therapeutic strategy for inflammatory bowel disease (IBD). Here, we established a novel probiotic cocktail to investigate its therapeutic role in acute colitis mice. During dextran sulfate sodium (DSS)-induced colitis, the...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.886061/full |
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| author | Yefei Zhu Yefei Zhu Yefei Zhu Yu Xu Yu Xu Xinyue Wang Leiping Rao Xuebing Yan Renyuan Gao Tongyi Shen Yuan Zhou Cheng Kong Longxiang Zhou |
| author_facet | Yefei Zhu Yefei Zhu Yefei Zhu Yu Xu Yu Xu Xinyue Wang Leiping Rao Xuebing Yan Renyuan Gao Tongyi Shen Yuan Zhou Cheng Kong Longxiang Zhou |
| author_sort | Yefei Zhu |
| collection | DOAJ |
| description | The modulation of the gut microbiome has been widely suggested as a promising therapeutic strategy for inflammatory bowel disease (IBD). Here, we established a novel probiotic cocktail to investigate its therapeutic role in acute colitis mice. During dextran sulfate sodium (DSS)-induced colitis, the mice were treated with the probiotic cocktail, fecal microbiota transplantation (FMT) from a healthy mice donor, or 5-aminosalicylic acid (5-ASA), respectively. The inflammatory responses were assessed by symptoms, serum inflammatory factors, and histological scoring. The intestinal barrier function was assessed by detecting tight junction proteins. Gut microbiota and its metabolites were further identified using 16S rDNA sequencing and a liquid chromatograph mass spectrometer (LC-MS/MS). Compared with FMT and 5-ASA treatment, the probiotic cocktail performed better in alleviating symptoms of colitis and decreasing disease activity score and mucosal inflammation. The probiotic cocktail also significantly decreased serum IL-17 level and increased JAM-1 expression in colon. The gut microbiota analysis confirmed that the beneficial effects of the probiotic cocktail were attributed to increasing anti-inflammatory bacteria Akkermansia, Bifidobacterium, and Blautia, while decreasing pro-inflammatory bacteria Parasutterella. The targeted metabolome analysis further indicated a rise in the production of Bifidobacterium-related short-chain fatty acids (SCFAs) such as propanoic acid and isobutyric acid after probiotics treatment. Taken together, the probiotic cocktail effectively alleviated intestinal inflammation through improving gut microbiota and metabolites in colitis mice, suggesting its great potential to be a novel therapeutic approach for IBD patients. |
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| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T17:52:14Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-2e7903f7ef584f1b920978cb43a5668a2022-12-22T02:36:39ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.886061886061Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis MiceYefei Zhu0Yefei Zhu1Yefei Zhu2Yu Xu3Yu Xu4Xinyue Wang5Leiping Rao6Xuebing Yan7Renyuan Gao8Tongyi Shen9Yuan Zhou10Cheng Kong11Longxiang Zhou12Research Institute of Intestinal Diseases, Tongji University, School of Medicine, Shanghai, ChinaDepartment of General Surgery, Jinshan Branch of Shanghai Sixth People’s Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, ChinaSchool of Medicine, Nantong University, Nantong, ChinaResearch Institute of Intestinal Diseases, Tongji University, School of Medicine, Shanghai, ChinaSchool of Medicine, Nantong University, Nantong, ChinaResearch Institute of Intestinal Diseases, Tongji University, School of Medicine, Shanghai, ChinaDepartment of General Surgery, Jinshan Branch of Shanghai Sixth People’s Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, ChinaDepartment of Oncology, the Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, ChinaResearch Institute of Intestinal Diseases, Tongji University, School of Medicine, Shanghai, ChinaResearch Institute of Intestinal Diseases, Tongji University, School of Medicine, Shanghai, ChinaSchool of Biological Engineering, Hefei Technology College, Hefei, ChinaResearch Institute of Intestinal Diseases, Tongji University, School of Medicine, Shanghai, ChinaDepartment of General Surgery, Jinshan Branch of Shanghai Sixth People’s Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, ChinaThe modulation of the gut microbiome has been widely suggested as a promising therapeutic strategy for inflammatory bowel disease (IBD). Here, we established a novel probiotic cocktail to investigate its therapeutic role in acute colitis mice. During dextran sulfate sodium (DSS)-induced colitis, the mice were treated with the probiotic cocktail, fecal microbiota transplantation (FMT) from a healthy mice donor, or 5-aminosalicylic acid (5-ASA), respectively. The inflammatory responses were assessed by symptoms, serum inflammatory factors, and histological scoring. The intestinal barrier function was assessed by detecting tight junction proteins. Gut microbiota and its metabolites were further identified using 16S rDNA sequencing and a liquid chromatograph mass spectrometer (LC-MS/MS). Compared with FMT and 5-ASA treatment, the probiotic cocktail performed better in alleviating symptoms of colitis and decreasing disease activity score and mucosal inflammation. The probiotic cocktail also significantly decreased serum IL-17 level and increased JAM-1 expression in colon. The gut microbiota analysis confirmed that the beneficial effects of the probiotic cocktail were attributed to increasing anti-inflammatory bacteria Akkermansia, Bifidobacterium, and Blautia, while decreasing pro-inflammatory bacteria Parasutterella. The targeted metabolome analysis further indicated a rise in the production of Bifidobacterium-related short-chain fatty acids (SCFAs) such as propanoic acid and isobutyric acid after probiotics treatment. Taken together, the probiotic cocktail effectively alleviated intestinal inflammation through improving gut microbiota and metabolites in colitis mice, suggesting its great potential to be a novel therapeutic approach for IBD patients.https://www.frontiersin.org/articles/10.3389/fcimb.2022.886061/fullgut microbiomegut barriershort-chain fatty acids (SCFAs)liquid chromatograph mass spectrometer (LC-MS/MS)inflammatory bowel disease (IBD)fecal microbiota transplantation (FMT) |
| spellingShingle | Yefei Zhu Yefei Zhu Yefei Zhu Yu Xu Yu Xu Xinyue Wang Leiping Rao Xuebing Yan Renyuan Gao Tongyi Shen Yuan Zhou Cheng Kong Longxiang Zhou Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice Frontiers in Cellular and Infection Microbiology gut microbiome gut barrier short-chain fatty acids (SCFAs) liquid chromatograph mass spectrometer (LC-MS/MS) inflammatory bowel disease (IBD) fecal microbiota transplantation (FMT) |
| title | Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice |
| title_full | Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice |
| title_fullStr | Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice |
| title_full_unstemmed | Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice |
| title_short | Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice |
| title_sort | probiotic cocktail alleviates intestinal inflammation through improving gut microbiota and metabolites in colitis mice |
| topic | gut microbiome gut barrier short-chain fatty acids (SCFAs) liquid chromatograph mass spectrometer (LC-MS/MS) inflammatory bowel disease (IBD) fecal microbiota transplantation (FMT) |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.886061/full |
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