Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppression
Atherosclerosis is a chronic inflammatory disease and the underlying cause of most cardiovascular diseases. Interleukin (IL)-1β facilitates early atherogenic lesion formation by increasing monocyte adhesion to endothelial cells via upregulation of adhesion molecules, including intercellular adhesion...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580821001400 |
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| author | Toshie Kihara Kohki Toriuchi Hiromasa Aoki Hiroki Kakita Yasumasa Yamada Mineyoshi Aoyama |
| author_facet | Toshie Kihara Kohki Toriuchi Hiromasa Aoki Hiroki Kakita Yasumasa Yamada Mineyoshi Aoyama |
| author_sort | Toshie Kihara |
| collection | DOAJ |
| description | Atherosclerosis is a chronic inflammatory disease and the underlying cause of most cardiovascular diseases. Interleukin (IL)-1β facilitates early atherogenic lesion formation by increasing monocyte adhesion to endothelial cells via upregulation of adhesion molecules, including intercellular adhesion molecule-1 (ICAM-1). MicroRNAs (miRNAs) have been shown to be associated with inflammatory conditions in the vascular system. The expression of circulating miR-1914–5p is reportedly downregulated in patients with cardiovascular diseases. However, the role of miR-1914–5p downregulation in IL-1β–induced endothelial cell dysfunction and the effect of miR-1914–5p on lesion formation remain unclear. Therefore, we investigated whether miR-1914–5p is associated with monocyte adhesion in human endothelial cells. IL-1β decreased miR-1914–5p expression in EA.hy926 cells. In addition, miR-1914–5p depletion enhanced ICAM-1 expression and monocyte adhesion in EA.hy926 cells. Moreover, miR-1914–5p mimic suppressed monocyte adhesion and ICAM-1 expression induced by IL-1β in endothelial cells. These results suggest that suppression of miR-1914–5p expression by IL-1β may be an important regulator in mediating monocyte adhesion in endothelial cells. Further investigation of miR-1914–5p may lead to the development of novel therapeutic strategies for atherosclerosis. |
| first_indexed | 2024-12-13T21:32:50Z |
| format | Article |
| id | doaj.art-2e795e2b498d42c2b78488dc1885f04f |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-13T21:32:50Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-2e795e2b498d42c2b78488dc1885f04f2022-12-21T23:30:45ZengElsevierBiochemistry and Biophysics Reports2405-58082021-09-0127101046Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppressionToshie Kihara0Kohki Toriuchi1Hiromasa Aoki2Hiroki Kakita3Yasumasa Yamada4Mineyoshi Aoyama5Department of Pathobiology, Nagoya City University Graduate School of Pharmaceutical Sciences, 3-1 Tanabe-dori, Mizoho-ku, Nagoya, Aichi, 467-8603, JapanDepartment of Pathobiology, Nagoya City University Graduate School of Pharmaceutical Sciences, 3-1 Tanabe-dori, Mizoho-ku, Nagoya, Aichi, 467-8603, JapanDepartment of Pathobiology, Nagoya City University Graduate School of Pharmaceutical Sciences, 3-1 Tanabe-dori, Mizoho-ku, Nagoya, Aichi, 467-8603, JapanDepartment of Pathobiology, Nagoya City University Graduate School of Pharmaceutical Sciences, 3-1 Tanabe-dori, Mizoho-ku, Nagoya, Aichi, 467-8603, Japan; Department of Perinatal and Neonatal Medicine, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, JapanDepartment of Perinatal and Neonatal Medicine, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, JapanDepartment of Pathobiology, Nagoya City University Graduate School of Pharmaceutical Sciences, 3-1 Tanabe-dori, Mizoho-ku, Nagoya, Aichi, 467-8603, Japan; Corresponding author. Department of Pathobiology Nagoya City University, Graduate School of Pharmaceutical Sciences, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan.Atherosclerosis is a chronic inflammatory disease and the underlying cause of most cardiovascular diseases. Interleukin (IL)-1β facilitates early atherogenic lesion formation by increasing monocyte adhesion to endothelial cells via upregulation of adhesion molecules, including intercellular adhesion molecule-1 (ICAM-1). MicroRNAs (miRNAs) have been shown to be associated with inflammatory conditions in the vascular system. The expression of circulating miR-1914–5p is reportedly downregulated in patients with cardiovascular diseases. However, the role of miR-1914–5p downregulation in IL-1β–induced endothelial cell dysfunction and the effect of miR-1914–5p on lesion formation remain unclear. Therefore, we investigated whether miR-1914–5p is associated with monocyte adhesion in human endothelial cells. IL-1β decreased miR-1914–5p expression in EA.hy926 cells. In addition, miR-1914–5p depletion enhanced ICAM-1 expression and monocyte adhesion in EA.hy926 cells. Moreover, miR-1914–5p mimic suppressed monocyte adhesion and ICAM-1 expression induced by IL-1β in endothelial cells. These results suggest that suppression of miR-1914–5p expression by IL-1β may be an important regulator in mediating monocyte adhesion in endothelial cells. Further investigation of miR-1914–5p may lead to the development of novel therapeutic strategies for atherosclerosis.http://www.sciencedirect.com/science/article/pii/S2405580821001400AtherosclerosisIL-1βmiR-1914–5pEndothelial cellsMonocyte adhesion |
| spellingShingle | Toshie Kihara Kohki Toriuchi Hiromasa Aoki Hiroki Kakita Yasumasa Yamada Mineyoshi Aoyama Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppression Biochemistry and Biophysics Reports Atherosclerosis IL-1β miR-1914–5p Endothelial cells Monocyte adhesion |
| title | Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppression |
| title_full | Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppression |
| title_fullStr | Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppression |
| title_full_unstemmed | Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppression |
| title_short | Interleukin-1β enhances cell adhesion in human endothelial cells via microRNA-1914–5p suppression |
| title_sort | interleukin 1β enhances cell adhesion in human endothelial cells via microrna 1914 5p suppression |
| topic | Atherosclerosis IL-1β miR-1914–5p Endothelial cells Monocyte adhesion |
| url | http://www.sciencedirect.com/science/article/pii/S2405580821001400 |
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