Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control study
Background: Endometriosis is a disease that affects women of reproductive age. This disease is characterized by the presence of endometrial-like tissues (endometrial or stromal glands) outside the uterus and shows significantly elevated prevalence in industrial regions. Additionally, an interaction...
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2018-10-01
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| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | http://journals.ssu.ac.ir/ijrmnew/article-1-1267-en.html |
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| author | Azam Babaki Ehsan Zare Mehrjardi Razieh Dehghani Firouzabadi Abbas Aflatoonian Seyed Morteza Seifati |
| author_facet | Azam Babaki Ehsan Zare Mehrjardi Razieh Dehghani Firouzabadi Abbas Aflatoonian Seyed Morteza Seifati |
| author_sort | Azam Babaki |
| collection | DOAJ |
| description | Background: Endometriosis is a disease that affects women of reproductive age. This disease is characterized by the presence of endometrial-like tissues (endometrial or stromal glands) outside the uterus and shows significantly elevated prevalence in industrial regions. Additionally, an interaction between genetics and environmental factors is assumed for the disease. Enzymes belonging to the cytochrome P450 (CYP) family are participated in detoxification process of a wide range of environmental toxins and carcinogens. Thereby, they are good link for the interaction. CYP1A1 which belong to cytochrome P450 (CYPs) superfamily, is a very important gene for the metabolism of carcinogens.
Objective: The aim of this study was to analyze the frequency of the MspI polymorphism of CYP1A1 gene and its relation to endometriosis.
Materials and Methods: Genomic DNA was isolated from 93 endometriosis women and 139 healthy controls. Genotyping was performed using polymerase chain reaction followed by restriction fragment length polymorphism analysis.
Results: Frequencies of the TT, TC, and CC genotype of CYP1A1 gene polymorphism in patients were 73.1%, 22.6%, and 4.3%, while frequencies in controls were 74.1%, 22.3%, and 3.6%, respectively. So there was no significant differences between the genotypes in two groups (p=0.961). Conclusion: According to our study, MspI polymorphism of CYP1A1 gene appears to be not associated with the risk of endometriosis in the studied population. However, additional studies, especially with larger sample size are needed to validate these findings. |
| first_indexed | 2024-12-13T16:03:15Z |
| format | Article |
| id | doaj.art-2e797d0d0d584875ac7475679f8a38b8 |
| institution | Directory Open Access Journal |
| issn | 2476-4108 2476-3772 |
| language | English |
| last_indexed | 2024-12-13T16:03:15Z |
| publishDate | 2018-10-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-2e797d0d0d584875ac7475679f8a38b82022-12-21T23:39:06ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722018-10-011610637640Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control studyAzam Babaki0Ehsan Zare Mehrjardi1Razieh Dehghani Firouzabadi2Abbas Aflatoonian3Seyed Morteza Seifati4 Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran Background: Endometriosis is a disease that affects women of reproductive age. This disease is characterized by the presence of endometrial-like tissues (endometrial or stromal glands) outside the uterus and shows significantly elevated prevalence in industrial regions. Additionally, an interaction between genetics and environmental factors is assumed for the disease. Enzymes belonging to the cytochrome P450 (CYP) family are participated in detoxification process of a wide range of environmental toxins and carcinogens. Thereby, they are good link for the interaction. CYP1A1 which belong to cytochrome P450 (CYPs) superfamily, is a very important gene for the metabolism of carcinogens. Objective: The aim of this study was to analyze the frequency of the MspI polymorphism of CYP1A1 gene and its relation to endometriosis. Materials and Methods: Genomic DNA was isolated from 93 endometriosis women and 139 healthy controls. Genotyping was performed using polymerase chain reaction followed by restriction fragment length polymorphism analysis. Results: Frequencies of the TT, TC, and CC genotype of CYP1A1 gene polymorphism in patients were 73.1%, 22.6%, and 4.3%, while frequencies in controls were 74.1%, 22.3%, and 3.6%, respectively. So there was no significant differences between the genotypes in two groups (p=0.961). Conclusion: According to our study, MspI polymorphism of CYP1A1 gene appears to be not associated with the risk of endometriosis in the studied population. However, additional studies, especially with larger sample size are needed to validate these findings.http://journals.ssu.ac.ir/ijrmnew/article-1-1267-en.htmlendometriosiscytochrome p-450 cyp1a1mspi endonuclease |
| spellingShingle | Azam Babaki Ehsan Zare Mehrjardi Razieh Dehghani Firouzabadi Abbas Aflatoonian Seyed Morteza Seifati Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control study International Journal of Reproductive BioMedicine endometriosis cytochrome p-450 cyp1a1 mspi endonuclease |
| title | Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control study |
| title_full | Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control study |
| title_fullStr | Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control study |
| title_full_unstemmed | Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control study |
| title_short | Relationship between MspI polymorphism of CYP1A1 gene and the risk of endometriosis in an Iranian population: A case-control study |
| title_sort | relationship between mspi polymorphism of cyp1a1 gene and the risk of endometriosis in an iranian population a case control study |
| topic | endometriosis cytochrome p-450 cyp1a1 mspi endonuclease |
| url | http://journals.ssu.ac.ir/ijrmnew/article-1-1267-en.html |
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