Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp.
Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and prot...
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| Format: | Article |
| Language: | English |
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Sociedade Botânica do Brasil
2015-12-01
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| Series: | Acta Botânica Brasílica |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567&lng=en&tlng=en |
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| author | Marlon Corrêa Pereira Diego Ismael Rocha Tomás Gomes Reis Veloso Olinto Liparini Pereira Dayana Maria Teodoro Francino Renata Maria Strozi Alves Meira Maria Catarina Megumi Kasuya |
| author_facet | Marlon Corrêa Pereira Diego Ismael Rocha Tomás Gomes Reis Veloso Olinto Liparini Pereira Dayana Maria Teodoro Francino Renata Maria Strozi Alves Meira Maria Catarina Megumi Kasuya |
| author_sort | Marlon Corrêa Pereira |
| collection | DOAJ |
| description | Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp.) promoted protocorm development until leaf production at 63 days. The protocorms are comprised of parenchyma cells delimited by a unistratified epidermis; the parenchyma cells of the upper part of the protocorms are smaller than those located more towards the base. Intact and digested pelotons were observed inside of protocorms implying that the seedlings were capable of mycotrophy. Additionally, the development of a bud primordium only occurred after colonization by fungus. This study suggests that C. glutiniferum has a preference for strains of Epulorhiza and that fungus digestion is essential to protocorm development. |
| first_indexed | 2024-12-12T19:41:39Z |
| format | Article |
| id | doaj.art-2e7eb0808ad44c4ab3481c975f214289 |
| institution | Directory Open Access Journal |
| issn | 1677-941X |
| language | English |
| last_indexed | 2024-12-12T19:41:39Z |
| publishDate | 2015-12-01 |
| publisher | Sociedade Botânica do Brasil |
| record_format | Article |
| series | Acta Botânica Brasílica |
| spelling | doaj.art-2e7eb0808ad44c4ab3481c975f2142892022-12-22T00:14:12ZengSociedade Botânica do BrasilActa Botânica Brasílica1677-941X2015-12-0129456757410.1590/0102-33062015abb0078S0102-33062015000400567Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp.Marlon Corrêa PereiraDiego Ismael RochaTomás Gomes Reis VelosoOlinto Liparini PereiraDayana Maria Teodoro FrancinoRenata Maria Strozi Alves MeiraMaria Catarina Megumi KasuyaCyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp.) promoted protocorm development until leaf production at 63 days. The protocorms are comprised of parenchyma cells delimited by a unistratified epidermis; the parenchyma cells of the upper part of the protocorms are smaller than those located more towards the base. Intact and digested pelotons were observed inside of protocorms implying that the seedlings were capable of mycotrophy. Additionally, the development of a bud primordium only occurred after colonization by fungus. This study suggests that C. glutiniferum has a preference for strains of Epulorhiza and that fungus digestion is essential to protocorm development.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567&lng=en&tlng=enCeratorhizaEpulorhizaorchid conservationseedling productionsymbiosis |
| spellingShingle | Marlon Corrêa Pereira Diego Ismael Rocha Tomás Gomes Reis Veloso Olinto Liparini Pereira Dayana Maria Teodoro Francino Renata Maria Strozi Alves Meira Maria Catarina Megumi Kasuya Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. Acta Botânica Brasílica Ceratorhiza Epulorhiza orchid conservation seedling production symbiosis |
| title | Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. |
| title_full | Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. |
| title_fullStr | Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. |
| title_full_unstemmed | Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. |
| title_short | Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. |
| title_sort | characterization of seed germination and protocorm development of cyrtopodium glutiniferum orchidaceae promoted by mycorrhizal fungi epulorhiza spp |
| topic | Ceratorhiza Epulorhiza orchid conservation seedling production symbiosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567&lng=en&tlng=en |
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