Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions
The occupational exposure to particles such as crystalline quartz and its impact on the respiratory tract have been studied extensively in recent years. For hazard assessment, the development of physiologically more relevant in-vitro models, i.e., air-liquid interface (ALI) cell cultures, has greatl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6412 |
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| author | Alexandra Friesen Susanne Fritsch-Decker Matthias Hufnagel Sonja Mülhopt Dieter Stapf Andrea Hartwig Carsten Weiss |
| author_facet | Alexandra Friesen Susanne Fritsch-Decker Matthias Hufnagel Sonja Mülhopt Dieter Stapf Andrea Hartwig Carsten Weiss |
| author_sort | Alexandra Friesen |
| collection | DOAJ |
| description | The occupational exposure to particles such as crystalline quartz and its impact on the respiratory tract have been studied extensively in recent years. For hazard assessment, the development of physiologically more relevant in-vitro models, i.e., air-liquid interface (ALI) cell cultures, has greatly progressed. Within this study, pulmonary culture models employing A549 and differentiated THP-1 cells as mono-and co-cultures were investigated. The different cultures were exposed to α-quartz particles (Min-U-Sil5) with doses ranging from 15 to 66 µg/cm<sup>2</sup> under submerged and ALI conditions and cytotoxicity as well as cytokine release were analyzed. No cytotoxicity was observed after ALI exposure. Contrarily, Min-U-Sil5 was cytotoxic at the highest dose in both submerged mono- and co-cultures. A concentration-dependent release of interleukin-8 was shown for both exposure types, which was overall stronger in co-cultures. Our findings showed considerable differences in the toxicological responses between ALI and submerged exposure and between mono- and co-cultures. A substantial influence of the presence or absence of serum in cell culture media was noted as well. Within this study, the submerged culture was revealed to be more sensitive. This shows the importance of considering different culture and exposure models and highlights the relevance of communication between different cell types for toxicological investigations. |
| first_indexed | 2024-03-09T23:35:48Z |
| format | Article |
| id | doaj.art-2e8097a5cdd24a09a359720123fd791e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:35:48Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2e8097a5cdd24a09a359720123fd791e2023-11-23T17:00:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312641210.3390/ijms23126412Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface ConditionsAlexandra Friesen0Susanne Fritsch-Decker1Matthias Hufnagel2Sonja Mülhopt3Dieter Stapf4Andrea Hartwig5Carsten Weiss6Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Institute of Applied Biosciences, 76131 Karlsruhe, GermanyKarlsruhe Institute of Technology (KIT), Institute of Biological and Chemical Systems, Biological Information Processing, 76344 Eggenstein-Leopoldshafen, GermanyDepartment of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Institute of Applied Biosciences, 76131 Karlsruhe, GermanyKarlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, GermanyDepartment of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Institute of Applied Biosciences, 76131 Karlsruhe, GermanyKarlsruhe Institute of Technology (KIT), Institute of Biological and Chemical Systems, Biological Information Processing, 76344 Eggenstein-Leopoldshafen, GermanyThe occupational exposure to particles such as crystalline quartz and its impact on the respiratory tract have been studied extensively in recent years. For hazard assessment, the development of physiologically more relevant in-vitro models, i.e., air-liquid interface (ALI) cell cultures, has greatly progressed. Within this study, pulmonary culture models employing A549 and differentiated THP-1 cells as mono-and co-cultures were investigated. The different cultures were exposed to α-quartz particles (Min-U-Sil5) with doses ranging from 15 to 66 µg/cm<sup>2</sup> under submerged and ALI conditions and cytotoxicity as well as cytokine release were analyzed. No cytotoxicity was observed after ALI exposure. Contrarily, Min-U-Sil5 was cytotoxic at the highest dose in both submerged mono- and co-cultures. A concentration-dependent release of interleukin-8 was shown for both exposure types, which was overall stronger in co-cultures. Our findings showed considerable differences in the toxicological responses between ALI and submerged exposure and between mono- and co-cultures. A substantial influence of the presence or absence of serum in cell culture media was noted as well. Within this study, the submerged culture was revealed to be more sensitive. This shows the importance of considering different culture and exposure models and highlights the relevance of communication between different cell types for toxicological investigations.https://www.mdpi.com/1422-0067/23/12/6412quartzpulmonary toxicityair-liquid interfaceco-culturecytotoxicityinflammation |
| spellingShingle | Alexandra Friesen Susanne Fritsch-Decker Matthias Hufnagel Sonja Mülhopt Dieter Stapf Andrea Hartwig Carsten Weiss Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions International Journal of Molecular Sciences quartz pulmonary toxicity air-liquid interface co-culture cytotoxicity inflammation |
| title | Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions |
| title_full | Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions |
| title_fullStr | Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions |
| title_full_unstemmed | Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions |
| title_short | Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions |
| title_sort | comparing α quartz induced cytotoxicity and interleukin 8 release in pulmonary mono and co cultures exposed under submerged and air liquid interface conditions |
| topic | quartz pulmonary toxicity air-liquid interface co-culture cytotoxicity inflammation |
| url | https://www.mdpi.com/1422-0067/23/12/6412 |
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