An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis
Summary: This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. H...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301726 |
| _version_ | 1818388201904013312 |
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| author | Hiroaki Kasashima Angeles Duran Tania Cid-Diaz Yotaro Kudo Maria T. Diaz-Meco Jorge Moscat |
| author_facet | Hiroaki Kasashima Angeles Duran Tania Cid-Diaz Yotaro Kudo Maria T. Diaz-Meco Jorge Moscat |
| author_sort | Hiroaki Kasashima |
| collection | DOAJ |
| description | Summary: This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD).For complete details on the use and execution of this protocol, please refer to Kudo et al. (2020). |
| first_indexed | 2024-12-14T04:22:05Z |
| format | Article |
| id | doaj.art-2e80bf2ebd594560aaadb1533afd5ce5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-14T04:22:05Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-2e80bf2ebd594560aaadb1533afd5ce52022-12-21T23:17:18ZengElsevierSTAR Protocols2666-16672020-12-0113100185An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver SteatosisHiroaki Kasashima0Angeles Duran1Tania Cid-Diaz2Yotaro Kudo3Maria T. Diaz-Meco4Jorge Moscat5Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; Sanford Burnham Prebys Medical Discovery Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA; Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, Osaka city, 545-8585, Japan; Department of Surgery, Kashiwara Municipal hospital, Kashiwara city, 582-0005, Japan; Corresponding authorDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10065, USASanford Burnham Prebys Medical Discovery Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USADepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; Sanford Burnham Prebys Medical Discovery Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10065, USA; Corresponding authorDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; Sanford Burnham Prebys Medical Discovery Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10065, USA; Corresponding authorSummary: This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD).For complete details on the use and execution of this protocol, please refer to Kudo et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301726Cell BiologyCancerMetabolismModel Organisms |
| spellingShingle | Hiroaki Kasashima Angeles Duran Tania Cid-Diaz Yotaro Kudo Maria T. Diaz-Meco Jorge Moscat An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis STAR Protocols Cell Biology Cancer Metabolism Model Organisms |
| title | An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis |
| title_full | An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis |
| title_fullStr | An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis |
| title_full_unstemmed | An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis |
| title_short | An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis |
| title_sort | orthotopic implantation mouse model of hepatocellular carcinoma with underlying liver steatosis |
| topic | Cell Biology Cancer Metabolism Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720301726 |
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