<i>Acacia longifolia</i>: A Host of Many Guests Even after Fire
<i>Acacia longifolia</i> is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Diversity |
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| Online Access: | https://www.mdpi.com/1424-2818/12/6/250 |
| _version_ | 1797564721867522048 |
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| author | Joana Guedes de Jesus Rogério Tenreiro Cristina Máguas Helena Trindade |
| author_facet | Joana Guedes de Jesus Rogério Tenreiro Cristina Máguas Helena Trindade |
| author_sort | Joana Guedes de Jesus |
| collection | DOAJ |
| description | <i>Acacia longifolia</i> is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured in nodules, including both nitrogen and non-nitrogen fixers. Furthermore, we aim to evaluate the effects of fire in nodulation and bacterial diversity on young acacias growing in unburnt and burnt zones, one year after the fire. For this, we used molecular approaches, M13 fingerprinting and 16S rRNA partial sequencing, to identify species/genera involved and δ<sup>15</sup>N isotopic composition in leaves and plant nodules. Nitrogen isotopic analyses in leaves suggest that in unburnt zones, nitrogen fixation contributes more to plant nitrogen content. Overall, <i>A. longifolia</i> seems to be promiscuous and despite <i>Bradyrhizobium</i> spp. dominance, <i>Paraburkholderia</i> spp. followed by <i>Pseudomonas</i> spp. was also found. Several species not previously reported as nitrogen-fixers were identified, proposing other functions besides ammonia acquisition. Our study shows that bacterial communities are different in nodules after fire. Fire seems to potentiate nodulation and drives symbiosis towards nitrogen-fixers. Taken together, a multifunctional community inside nodules is pointed out which potentiate <i>A. longifolia</i> invasiveness and adaptation. |
| first_indexed | 2024-03-10T19:00:59Z |
| format | Article |
| id | doaj.art-2e813cd411444ee8876bfe93da0d1e78 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T19:00:59Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-2e813cd411444ee8876bfe93da0d1e782023-11-20T04:23:01ZengMDPI AGDiversity1424-28182020-06-0112625010.3390/d12060250<i>Acacia longifolia</i>: A Host of Many Guests Even after FireJoana Guedes de Jesus0Rogério Tenreiro1Cristina Máguas2Helena Trindade3Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, PortugalBiosystems and Integrative Sciences Institute (BioISI), Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, PortugalCentre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, PortugalCentro de Estudos do Ambiente e do Mar (CESAM), Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, Portugal<i>Acacia longifolia</i> is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured in nodules, including both nitrogen and non-nitrogen fixers. Furthermore, we aim to evaluate the effects of fire in nodulation and bacterial diversity on young acacias growing in unburnt and burnt zones, one year after the fire. For this, we used molecular approaches, M13 fingerprinting and 16S rRNA partial sequencing, to identify species/genera involved and δ<sup>15</sup>N isotopic composition in leaves and plant nodules. Nitrogen isotopic analyses in leaves suggest that in unburnt zones, nitrogen fixation contributes more to plant nitrogen content. Overall, <i>A. longifolia</i> seems to be promiscuous and despite <i>Bradyrhizobium</i> spp. dominance, <i>Paraburkholderia</i> spp. followed by <i>Pseudomonas</i> spp. was also found. Several species not previously reported as nitrogen-fixers were identified, proposing other functions besides ammonia acquisition. Our study shows that bacterial communities are different in nodules after fire. Fire seems to potentiate nodulation and drives symbiosis towards nitrogen-fixers. Taken together, a multifunctional community inside nodules is pointed out which potentiate <i>A. longifolia</i> invasiveness and adaptation.https://www.mdpi.com/1424-2818/12/6/250climate changeinvasive speciesnodulationsymbiosis<i>Bradyrhizobium</i> spp. |
| spellingShingle | Joana Guedes de Jesus Rogério Tenreiro Cristina Máguas Helena Trindade <i>Acacia longifolia</i>: A Host of Many Guests Even after Fire Diversity climate change invasive species nodulation symbiosis <i>Bradyrhizobium</i> spp. |
| title | <i>Acacia longifolia</i>: A Host of Many Guests Even after Fire |
| title_full | <i>Acacia longifolia</i>: A Host of Many Guests Even after Fire |
| title_fullStr | <i>Acacia longifolia</i>: A Host of Many Guests Even after Fire |
| title_full_unstemmed | <i>Acacia longifolia</i>: A Host of Many Guests Even after Fire |
| title_short | <i>Acacia longifolia</i>: A Host of Many Guests Even after Fire |
| title_sort | i acacia longifolia i a host of many guests even after fire |
| topic | climate change invasive species nodulation symbiosis <i>Bradyrhizobium</i> spp. |
| url | https://www.mdpi.com/1424-2818/12/6/250 |
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