UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) Extract
<i>Rabelera holostea</i> (L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as <i>Stellaria holostea</i> L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowada...
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/1274 |
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| author | Jelena S. Katanić Stanković Jelena Đorović Jovanović Danijela Mišić Uroš Gašić Stefanie Nikles Zoran Marković Rudolf Bauer |
| author_facet | Jelena S. Katanić Stanković Jelena Đorović Jovanović Danijela Mišić Uroš Gašić Stefanie Nikles Zoran Marković Rudolf Bauer |
| author_sort | Jelena S. Katanić Stanković |
| collection | DOAJ |
| description | <i>Rabelera holostea</i> (L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as <i>Stellaria holostea</i> L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowadays it is considered as a weed, but earlier it was often used raw in salads or for the treatment of various inflammatory disorders. The goal of this study was to determine the constituents of the methanol extract of <i>R. holostea</i> aerial parts and its biological potential in terms of antioxidant, antimicrobial, and anti-inflammatory properties. Until now, the constituents and biological activities of this plant were not reported in detail. A comprehensive phytochemical profiling of the extract has shown that phenolic acids, such as ferulic, chlorogenic, and <i>p</i>-coumaric acid, flavonoids and flavonoid glucosides, such as chrysoeriol, rutin, and naringin, are the most abundant compounds. The antioxidant activity of <i>R. holostea</i> extract towards DPPH and ABTS radicals, but also the total antioxidant capacity and the inhibition of lipid peroxidation were moderate. The antimicrobial potential was pronounced mostly towards some fungi such as <i>F. oxysporum</i> (MIC 1.25 mg/mL), whereas the capacity of <i>R. holostea</i> to affect the growth of bacteria was much less pronounced. <i>R. holostea</i> extract was most inclined to anti-inflammatory activity. At a concentration of 50 µg/mL, it significantly inhibited both cyclooxygenase enzymes (COX-1 and COX-2) by 71.24% and 72.83%, respectively. Molecular docking studies indicated that chlorogenic acid and chrysoeriol are the main contributors to COX-1 and COX-2 inhibitory activity. |
| first_indexed | 2024-03-11T09:33:08Z |
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| id | doaj.art-2e81c160166e4910a7f8a3a98885bb12 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:33:08Z |
| publishDate | 2023-01-01 |
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| series | Molecules |
| spelling | doaj.art-2e81c160166e4910a7f8a3a98885bb122023-11-16T17:30:06ZengMDPI AGMolecules1420-30492023-01-01283127410.3390/molecules28031274UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) ExtractJelena S. Katanić Stanković0Jelena Đorović Jovanović1Danijela Mišić2Uroš Gašić3Stefanie Nikles4Zoran Marković5Rudolf Bauer6Department of Science, Institute for Information Technologies Kragujevac, University of Kragujevac, Jovana Cvijića bb, 34000 Kragujevac, SerbiaDepartment of Science, Institute for Information Technologies Kragujevac, University of Kragujevac, Jovana Cvijića bb, 34000 Kragujevac, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Pharmacognosy, Institute for Pharmaceutical Sciences, University of Graz, Beethovenstrasse 8, 8010 Graz, AustriaDepartment of Science, Institute for Information Technologies Kragujevac, University of Kragujevac, Jovana Cvijića bb, 34000 Kragujevac, SerbiaDepartment of Pharmacognosy, Institute for Pharmaceutical Sciences, University of Graz, Beethovenstrasse 8, 8010 Graz, Austria<i>Rabelera holostea</i> (L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as <i>Stellaria holostea</i> L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowadays it is considered as a weed, but earlier it was often used raw in salads or for the treatment of various inflammatory disorders. The goal of this study was to determine the constituents of the methanol extract of <i>R. holostea</i> aerial parts and its biological potential in terms of antioxidant, antimicrobial, and anti-inflammatory properties. Until now, the constituents and biological activities of this plant were not reported in detail. A comprehensive phytochemical profiling of the extract has shown that phenolic acids, such as ferulic, chlorogenic, and <i>p</i>-coumaric acid, flavonoids and flavonoid glucosides, such as chrysoeriol, rutin, and naringin, are the most abundant compounds. The antioxidant activity of <i>R. holostea</i> extract towards DPPH and ABTS radicals, but also the total antioxidant capacity and the inhibition of lipid peroxidation were moderate. The antimicrobial potential was pronounced mostly towards some fungi such as <i>F. oxysporum</i> (MIC 1.25 mg/mL), whereas the capacity of <i>R. holostea</i> to affect the growth of bacteria was much less pronounced. <i>R. holostea</i> extract was most inclined to anti-inflammatory activity. At a concentration of 50 µg/mL, it significantly inhibited both cyclooxygenase enzymes (COX-1 and COX-2) by 71.24% and 72.83%, respectively. Molecular docking studies indicated that chlorogenic acid and chrysoeriol are the main contributors to COX-1 and COX-2 inhibitory activity.https://www.mdpi.com/1420-3049/28/3/1274<i>Rabelera holostea</i> (L.) M. T. Sharples & E. A. Tripp<i>Stellaria holostea</i>greater stitchwortUHPLCphenolicsbioactivity |
| spellingShingle | Jelena S. Katanić Stanković Jelena Đorović Jovanović Danijela Mišić Uroš Gašić Stefanie Nikles Zoran Marković Rudolf Bauer UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) Extract Molecules <i>Rabelera holostea</i> (L.) M. T. Sharples & E. A. Tripp <i>Stellaria holostea</i> greater stitchwort UHPLC phenolics bioactivity |
| title | UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) Extract |
| title_full | UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) Extract |
| title_fullStr | UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) Extract |
| title_full_unstemmed | UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) Extract |
| title_short | UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of <i>Rabelera holostea</i> (Greater Stitchwort) Extract |
| title_sort | uhplc ms phytochemical profiling and insight into bioactivity of i rabelera holostea i greater stitchwort extract |
| topic | <i>Rabelera holostea</i> (L.) M. T. Sharples & E. A. Tripp <i>Stellaria holostea</i> greater stitchwort UHPLC phenolics bioactivity |
| url | https://www.mdpi.com/1420-3049/28/3/1274 |
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