Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis
Health risks caused by stings from <i>Vespa velutina nigrithorax</i> (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac (VS) based on Sequential...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/4/266 |
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| author | Manuela Alonso-Sampedro Xesús Feás Susana Belén Bravo María Pilar Chantada-Vázquez Carmen Vidal |
| author_facet | Manuela Alonso-Sampedro Xesús Feás Susana Belén Bravo María Pilar Chantada-Vázquez Carmen Vidal |
| author_sort | Manuela Alonso-Sampedro |
| collection | DOAJ |
| description | Health risks caused by stings from <i>Vespa velutina nigrithorax</i> (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac (VS) based on Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS). The study also performed proteomic quantitative analysis and examined the biological pathways and molecular functions of the proteins in the VS of VV gynes (i.e., future queens [SQ]) and workers [SW]. The total protein content per VS was significantly higher in the SW than in the SQ (274 ± 54 µg/sac vs. 175 ± 22 µg/sac; <i>p</i> = 0.02). We quantified a total of 228 proteins in the VS, belonging to 7 different classes: <i>Insecta</i> (<i>n</i> = 191); <i>Amphibia</i> and <i>Reptilia</i> (<i>n</i> = 20); <i>Bacilli</i>, <i>γ-Proteobacteria</i> and <i>Pisoniviricetes</i> (<i>n</i> = 12); and <i>Arachnida</i> (<i>n</i> = 5). Among the 228 identified proteins, 66 showed significant differential expression between SQ and SW. The potential allergens hyaluronidase A, venom antigen 5 and phospholipase A1 were significantly downregulated in the SQ venom. |
| first_indexed | 2024-03-11T04:27:52Z |
| format | Article |
| id | doaj.art-2e826ef27e0f476491312003163f567d |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-11T04:27:52Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-2e826ef27e0f476491312003163f567d2023-11-17T21:38:46ZengMDPI AGToxins2072-66512023-04-0115426610.3390/toxins15040266Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS AnalysisManuela Alonso-Sampedro0Xesús Feás1Susana Belén Bravo2María Pilar Chantada-Vázquez3Carmen Vidal4Fundación Instituto de Investigación Sanitaria de Santiago de Compostela (FIDIS), Hospital Clínico, 15706 Santiago de Compostela, SpainFundación Instituto de Investigación Sanitaria de Santiago de Compostela (FIDIS), Hospital Clínico, 15706 Santiago de Compostela, SpainFundación Instituto de Investigación Sanitaria de Santiago de Compostela (FIDIS), Hospital Clínico, 15706 Santiago de Compostela, SpainFundación Instituto de Investigación Sanitaria de Santiago de Compostela (FIDIS), Hospital Clínico, 15706 Santiago de Compostela, SpainFundación Instituto de Investigación Sanitaria de Santiago de Compostela (FIDIS), Hospital Clínico, 15706 Santiago de Compostela, SpainHealth risks caused by stings from <i>Vespa velutina nigrithorax</i> (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac (VS) based on Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS). The study also performed proteomic quantitative analysis and examined the biological pathways and molecular functions of the proteins in the VS of VV gynes (i.e., future queens [SQ]) and workers [SW]. The total protein content per VS was significantly higher in the SW than in the SQ (274 ± 54 µg/sac vs. 175 ± 22 µg/sac; <i>p</i> = 0.02). We quantified a total of 228 proteins in the VS, belonging to 7 different classes: <i>Insecta</i> (<i>n</i> = 191); <i>Amphibia</i> and <i>Reptilia</i> (<i>n</i> = 20); <i>Bacilli</i>, <i>γ-Proteobacteria</i> and <i>Pisoniviricetes</i> (<i>n</i> = 12); and <i>Arachnida</i> (<i>n</i> = 5). Among the 228 identified proteins, 66 showed significant differential expression between SQ and SW. The potential allergens hyaluronidase A, venom antigen 5 and phospholipase A1 were significantly downregulated in the SQ venom.https://www.mdpi.com/2072-6651/15/4/266Asian hornet<i>Vespa velutina nigrithorax</i><i>Vespa</i> spp.proteomicsSWATH-MShymenopteran |
| spellingShingle | Manuela Alonso-Sampedro Xesús Feás Susana Belén Bravo María Pilar Chantada-Vázquez Carmen Vidal Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis Toxins Asian hornet <i>Vespa velutina nigrithorax</i> <i>Vespa</i> spp. proteomics SWATH-MS hymenopteran |
| title | Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
| title_full | Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
| title_fullStr | Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
| title_full_unstemmed | Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
| title_short | Proteomics of <i>Vespa velutina nigrithorax</i> Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
| title_sort | proteomics of i vespa velutina nigrithorax i venom sac queens and workers a quantitative swath ms analysis |
| topic | Asian hornet <i>Vespa velutina nigrithorax</i> <i>Vespa</i> spp. proteomics SWATH-MS hymenopteran |
| url | https://www.mdpi.com/2072-6651/15/4/266 |
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