Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies.
The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the microbial communities in human, animal, and environmental health. Yet, use of next generation sequencing to perform 16S rRNA g...
Main Authors: | Patrick D Schloss, Dirk Gevers, Sarah L Westcott |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3237409?pdf=render |
Similar Items
-
Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies
by: Schloss, Patrick D., et al.
Published: (2012) -
The Impact of DNA Polymerase and Number of Rounds of Amplification in PCR on 16S rRNA Gene Sequence Data
by: Marc A. Sze, et al.
Published: (2019-06-01) -
De novo clustering methods outperform reference-based methods for assigning 16S rRNA gene sequences to operational taxonomic units
by: Sarah L. Westcott, et al.
Published: (2015-12-01) -
Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system
by: Patrick D. Schloss, et al.
Published: (2016-03-01) -
The effects of alignment quality, distance calculation method, sequence filtering, and region on the analysis of 16S rRNA gene-based studies.
by: Patrick D Schloss
Published: (2010-01-01)