Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil
Aphelenchoides pseudogoodeyi has recently been reported in association with seeds of forage grasses and rice in Brazil and senescent strawberry plants, in the United States. This nematode is likely a mycophagous species; however, so far, its pathogenicity potential to plants is unclear. This study a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2021-02-01
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| Series: | Helminthologia |
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| Online Access: | https://doi.org/10.2478/helm-2021-0003 |
| _version_ | 1797758689844658176 |
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| author | Santos E. C. Silva I. L. Da Rosa R. O. Oliveira C. M. G. Buonicontro D. S. |
| author_facet | Santos E. C. Silva I. L. Da Rosa R. O. Oliveira C. M. G. Buonicontro D. S. |
| author_sort | Santos E. C. |
| collection | DOAJ |
| description | Aphelenchoides pseudogoodeyi has recently been reported in association with seeds of forage grasses and rice in Brazil and senescent strawberry plants, in the United States. This nematode is likely a mycophagous species; however, so far, its pathogenicity potential to plants is unclear. This study aimed to verify the pathogenicity of A. pseudogoodeyi to two species of ornamental plants. The experiments were conducted by inoculating A. pseudogoodeyi onto Bird’s-Nest Fern (Asplenium nidus) and Oriental Lily (Lilium speciosum) leaves, using two inoculation methods (with and without injury). After 40 days of inoculation (DAI) in Bird’s-Nest Fern and 5, 10, 20 and 40 DAI in Oriental Lily, the pathogenicity and the host efficiency were evaluated by symptoms observation and by severity, final nematode population and reproductive factor (RF), respectively. Additionally, a histopathological study was performed by inoculating A. pseudogoodeyi onto Bird’s-Nest Fern for observing anatomical alterations. A. pseudogoodeyi was able to cause local necrotic lesions on both Bird’s-Nest Fern and Oriental Lily leaves. However, the presence of injury was essential to enable A. pseudogoodeyi to penetrate and cause those symptoms in both plant species. Also, the total population of A. pseudogoodeyi decreased drastically over time and RF was <1, which characterized these species as poor-host or resistant plants. A. pseudogoodeyi penetrated into the foliar tissue and induced a total destruction of the mesophyll and collapse of the cells, with the formation of large intercellular spaces. It is concluded that A. pseudogoodeyi is an opportunistic pathogen as injury was required to induce symptoms in Bird’s-Nest Fern and Oriental Lily. |
| first_indexed | 2024-03-12T18:33:43Z |
| format | Article |
| id | doaj.art-2e85eb69f6dc484b8b8c5df54f4ec183 |
| institution | Directory Open Access Journal |
| issn | 0440-6605 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T18:33:43Z |
| publishDate | 2021-02-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-2e85eb69f6dc484b8b8c5df54f4ec1832023-08-02T08:09:27ZengSciendoHelminthologia0440-66051336-90832021-02-01581748410.2478/helm-2021-0003Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in BrazilSantos E. C.0Silva I. L. Da1Rosa R. O.2Oliveira C. M. G.3Buonicontro D. S.4Viçosa Federal University, Department of Plant Pathology, Laboratory of Nematology, 36570-900, Viçosa, MG, BrazilViçosa Federal University, Department of Plant Biology, Plant Anatomy Laboratory, 36570-900, MG, BrazilViçosa Federal University, Department of Plant Pathology, Laboratory of Nematology, 36570-900, Viçosa, MG, BrazilBiological Institute, POBox 70, 13001-970, Campinas, SP, BrazilViçosa Federal University, Department of Plant Pathology, Laboratory of Nematology, 36570-900, Viçosa, MG, BrazilAphelenchoides pseudogoodeyi has recently been reported in association with seeds of forage grasses and rice in Brazil and senescent strawberry plants, in the United States. This nematode is likely a mycophagous species; however, so far, its pathogenicity potential to plants is unclear. This study aimed to verify the pathogenicity of A. pseudogoodeyi to two species of ornamental plants. The experiments were conducted by inoculating A. pseudogoodeyi onto Bird’s-Nest Fern (Asplenium nidus) and Oriental Lily (Lilium speciosum) leaves, using two inoculation methods (with and without injury). After 40 days of inoculation (DAI) in Bird’s-Nest Fern and 5, 10, 20 and 40 DAI in Oriental Lily, the pathogenicity and the host efficiency were evaluated by symptoms observation and by severity, final nematode population and reproductive factor (RF), respectively. Additionally, a histopathological study was performed by inoculating A. pseudogoodeyi onto Bird’s-Nest Fern for observing anatomical alterations. A. pseudogoodeyi was able to cause local necrotic lesions on both Bird’s-Nest Fern and Oriental Lily leaves. However, the presence of injury was essential to enable A. pseudogoodeyi to penetrate and cause those symptoms in both plant species. Also, the total population of A. pseudogoodeyi decreased drastically over time and RF was <1, which characterized these species as poor-host or resistant plants. A. pseudogoodeyi penetrated into the foliar tissue and induced a total destruction of the mesophyll and collapse of the cells, with the formation of large intercellular spaces. It is concluded that A. pseudogoodeyi is an opportunistic pathogen as injury was required to induce symptoms in Bird’s-Nest Fern and Oriental Lily.https://doi.org/10.2478/helm-2021-0003aphelenchoididaebird’s-nest fernfoliar nematodelilium speciosum |
| spellingShingle | Santos E. C. Silva I. L. Da Rosa R. O. Oliveira C. M. G. Buonicontro D. S. Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil Helminthologia aphelenchoididae bird’s-nest fern foliar nematode lilium speciosum |
| title | Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil |
| title_full | Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil |
| title_fullStr | Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil |
| title_full_unstemmed | Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil |
| title_short | Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil |
| title_sort | pathogenicity of aphelenchoides pseudogoodeyi on the ornamental plants asplenium nidus and lilium speciosum in brazil |
| topic | aphelenchoididae bird’s-nest fern foliar nematode lilium speciosum |
| url | https://doi.org/10.2478/helm-2021-0003 |
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